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49 protocols using sodium hydroxide (naoh)

1

Synthesis of Calcium-Copper Phosphate

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γ-PGA with a molecular weight of about 1.2 MDa was provided by Vedan Enterprise Corporation (Taichung, Taiwan). Calcium nitrate tetrahydrate and copper nitrate trihydrate were purchased from Showa Chemical Co. (Tokyo, Japan). Diammonium phosphate, ammonia solution and nitric acid were obtained from Katayama Chemical Co. (Osaka, Japan). Sodium hydroxide was from Riedel-de Haën (Seelze, Germany). Copper standard solution (1000 mg/L) was bought from Merck (Darmstadt, Germany). All chemicals were used as received without further purification.
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2

Synthesis of Cobalt-based Compounds

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Magnesium nitrate hexahydrate (99%), methanol (99%), cobalt nitrate hexahydrate (98%), KOH (97%), ammonium peroxy disulphate (98%), acetone (99%), and HCl (35.5%) were bought from Merck, Darmstadt, Germany while sodium hydroxide (97%), aniline (99%) and ethanol (99%) were purchased from Riedel-de Haen (Seelze, Germany). Voriconazole (98%) was obtained from Sigma Aldrich (St. Louis, MO, USA).
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3

Synthesis of TiO2 Nanotubes

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The synthesis of TiO2 NTs was based on TiO2 P25 (75% Anatase, 25% Rutile
Degussa) and is depicted in Figure 1. The nitric acid (HNO3), sodium hydroxide
(NaOH) and cesium hydroxide (CsOH) were provided by Riedel de Haën.
Cesium nitrate (CsNO3) was purchased from Fluka. Potassium
hydrogen phthalate (KHP) used for the NaOH standardization was obtained
from Sigma-Aldrich. Five standard buffers, pH = 2, 4, 6, 8, and 10
that were purchased from Reidel-de Haën were used for the potentiometric
measurements, i.e., electrode calibration. All solutions were prepared
by dissolving them in CO2-free deionized water. All chemicals
were used as-received without purification.
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4

Synthesis of Gold Nanoparticles

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BSA was purchased from Sigma-Aldrich (A7906-10g). Hydrogen tetrachloroaurate(III) trihydrate (HAuCl4) and isonicotinic acid hydrazide were obtained from Alfa Aesar. Rifampicin was purchased from Sigma Aldrich (R3501-1g), pyrazinamide was obtained from Acros Organics, and ethambutol dihydrochloride was obtained from LKT Laboratories, Inc. Sodium hydroxide was obtained from Riedel–de Haen. All of the solvents and chemicals were used without further purification. Aqueous solutions were prepared using deionized water (DI) with a resistivity of 18.20 MΩ cm.
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5

Preparation of Zwitterionic Polymer for Biomedical Applications

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4‐Mercaptobenzoic acid (technical grade 90%, C7H6O2S, chemical abstracts service (CAS) No. 1074368), iodine plates (CAS No. 7553562), ethanol (C2H5OH, CAS No. 64175), trimethylamine (C3H9N, CAS No. 75‐50‐3), sodium thiosulfate (Na2S2O3, CAS No. 7772987), N,N‐dimethylacetamide (C4H9NO, CAS No. 127195), l‐cystine (C6H12N2O4S2, CAS No. 56893), methacrylic anhydride (C8H10O3, CAS No. 760930), thionyl chloride (SOCl2, CAS No, 7719097), and [2‐(methacryloyloxy)ethyl]dimethyl‐(3‐sulfopropyl)ammonium hydroxide (SBMA, CAS No. 3637261) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Hydrochloric acid (HCl; CAS No. 7637010) and sodium hydroxide (NaOH; CAS No. 1310732) were purchased from Riedel‐de Haën (Germany). Ammonium persulfate ((NH4)2S2O8) was purchased from J.T. Baker (New Jersey, USA).
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6

Chitosan-Alginate Hydrogel Synthesis

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Chitosan (deacetylation degree ≥95%) was purchased from Golden-Shell Biochemical Co., Ltd. (Zhejiang, China) and sodium alginate from Manutex RSX, Kelco International, Ltd. (Portugal). 5-Aminosalicylic acid with a mass fraction purity of 99% was purchased from Sigma-Aldrich (St. Louis, USA). Commercial glycomacropeptide (GMP) was obtained from Davisco Food International, Inc. (Le Sueur, MN, USA). Sodium hydroxide and hydrochloric acid at 37% were obtained from Riedel-de Haen (Germany). Lactic acid (90%) was purchased from Merck (Germany). Hanks balanced salt solution (HBSS) was obtained from Invitrogen (Gibco, Invitrogen Corporation, Paisley, UK). Polystyrene nanoparticles were purchased from Polysciences, Inc. (Warrington, PA, USA). All samples were prepared with distilled water purified to a resistance of 15 M cm.
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7

Protein Purification and Characterization Protocol

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Lactoferrin-Lf was purchased from DMV International (USA) and Glycomacropeptide-GMP was kindly offered by Davisco Food International, INC. (Le Sueur, USA). To prepare the samples, it was used deionized water purified to a resistance of 15 MU, Millipore Corp. (France).
Hydrochloric acid was purchased from Panreac, Spain and sodium hydroxide was obtained from Riedel-de Haen (Germany). Hydrophilic model compound, caffeine was purchased from VWR (USA) and Amicon ® Ultra-0.5 centrifugal filter of 3 kDa and 8 kDa devices from, Millipore Corp. (Ireland) were used. Lipophilic model compound, curcumin was purchased from SigmaeAldrich, St. Louis and pure ethanol was purchased from Panreac (Barcelona, Spain). Fluorescein isothiocyanate (FITC) was purchased from Fluka (Germany). Standard marker proteins from PageRuler™ Broad Range Unstained Protein Ladder, Lot ##002252 was purchased from Thermo Scientific (Lithuania).
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8

Linseed-based Moxifloxacin Delivery System

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Linseeds were procured from Pansari inn, Pakistan. Linseeds were de-dusted and cleaned from physical impurities, and stored in an airtight container at ambient temperature. Microcrystalline cellulose (Avicel® PH 102) and HPMC K100M were procured from Merck, Germany. Moxifloxacin used in this research was according to the specification of United States Pharmacopoeia (USP). The KCl, NaOH, n-hexane, potassium dihydrogen phosphate and HCl were purchased from Riedel-de Haën, Germany. Magnesium stearate, polyvinylpyrrolidone K30 (PVP K30), talcum, sodium bicarbonate and β-cyclodextrin were procured from Sigma-Aldrich, USA. Linseeds hydrogel (LSH) was separated from linseeds using a nylon mesh. Deionized water was used for the entire study.
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9

Preparation of Acetylsalicylate Compounds

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D,L-lysine acetylsalicylate ⋅ glycine (BAY 81-8781, LASAG; MW = 363.5), acetylsalicylate (ASA; MW = 180.1) and salicylate (SA; MW = 160.1) were provided by Bayer HealthCare AG (Wuppertal, Germany). For in vitro studies, directly prior to the experiment a 10 mM stock solution was prepared by dissolving 10.9 mg BAY 81-8781 in 3 mL BA-medium. For ASA, a 10 mM stock solution was prepared by dissolving 5.4 mg ASA in 1 mL PBS by 5 min incubation at 37°C. pH-value was adjusted to 7.4 with 1N NaOH (Riedel-de-Haën, Germany). Thereafter, 2 mL BA-medium were added to the ASA/PBS-solution. 4.8 mg SA was dissolved in 3 mL BA-medium to obtain a 10 mM stock solution. For in vivo studies, different amounts of BAY 81-8781 were dissolved immediately prior to experiment in always 35 mL ddH2O (0.3% = 0.105 g; 0.1% = 0.35 g; 3% = 1.05%; 10% = 3.50 g and 30% = 10.5 g). As a source for oseltamivir, the complete contents of a Tamiflu® capsule was dissolved in 75 mL ddH2O to get a 1 mg/mL concentration.
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10

Hydrogel Carrier Synthesis for Drug Delivery

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Seeds of S. spinosa were brought from the local market of Sargodha, Pakistan (in May–June) and their taxonomy was verified by a botanist, Dr Hassan Sher from the Department of Botany, University of Swat, Mingora, Pakistan. The methacrylic acid (MAA, monomer; 99.0%), potassium persulfate (KPS, initiator; 99.0%), and N,N-methylenebisacrylamide (MBA, cross-linker; 99.5%) were purchased from Sigma-Aldrich, Germany. Potassium dihydrogen phosphate (KH2PO4; 99.0%), NaOH (98.0%), KCl (99.0%), ethanol (95.0%), and n-hexane (95.0%) were acquired from Riedel-de Haen, Germany. Venlafaxine HCl (United States Pharmacopeia (USP) standard) was used as a model drug for in vitro drug release studies from the synthesized hydrogel carrier. For swelling and drug release studies, the buffers of pH 7.4, 6.8, 4.5, and 1.2 were prepared according to the procedure given in USP. All other reagents and chemical ingredients were of lab-grade. Deionized water (DW) was used during the whole research work.
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