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Measurement software

Manufactured by Fujifilm

Measurement Software is a laboratory analysis tool that provides measurement and data processing capabilities. It offers features for data collection, analysis, and visualization to support scientific research and quality control tasks.

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4 protocols using measurement software

1

Echocardiographic Assessment of Cardiac Function

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Echocardiography was performed using Vevo 3100 High‐Resolution Imaging System (VisualSonics Inc, Toronto, Canada). Mice were sedated with 3% isoflurane and heart rate was maintained at 450–460 beats per minute by adjusting isoflurane concentration 1–1.5%. Short axis B‐ and M‐mode 2D echocardiograms were recorded through the anterior and posterior LV walls at the level of the papillary muscle. Left ventricular internal diameter at end‐systole (LVIDs) and end‐diastole (LVIDd), left ventricular posterior wall thickness, end‐systole (LVPWs) and end‐diastole (LVPWd), and fractional shortening (FS) and ejection fraction (EF) were measured by the VisualSonics Measurement Software.
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2

Echocardiographic Assessment of Murine Cardiac Function

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Two-dimensional-guided M-mode echocardiography was performed in mice at 23 weeks of age using the Vevo 1100 imaging system equipped with a 30-MHz high-frequency linear transducer (Visual Sonics, Toronto, Canada). Briefly, mice were lightly sedated with chloral hydrate (4%, intraperitoneal injection), and the prethoracic skin hair was shaved using a commercially available depilatory cream. A guided M-mode echocardiogram was recorded through the anterior and posterior left ventricular (LV) walls at 21 frames/s. Images were obtained at the level of the papillary muscle tips, and then measurements were performed to obtain the LV anterior wall at diastole (LVAW, d) and systole (LVAW, s), the LV posterior wall at diastole (LVPW, d) and systole (LVPW, s), the LV internal dimension at diastole (LVID, d) and systole (LVID, s), and the interventricular septum thickness at diastole (IVS, d) and systole (IVS, s) in M-mode. Percentage fractional shortening (FS%) and ejection fraction (EF%) were calculated via the Visual Sonics Measurement Software.
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3

Echocardiographic Analysis of Murine Cardiac Function

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Echocardiography was performed on anesthetized (1.5% isoflurane) mice using the Vevo 770 High-Resolution Imaging System (VisualSonics Inc.) with a 35-MHz high-frequency linear transducer as previously described [6 ]. Left ventricle (LV) internal dimension (LVID; in mm), intraventricular septal thickness (IVS; in mm), and LV posterior wall thickness (LVPW; in mm) were measured. LV percent fractional shortening FS (%) was calculated via VisualSonics Measurement Software.
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4

Two-dimensional Echocardiography of Mice

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Two-dimensional-guided M-mode echocardiography of the mice was performed on days 0, 7, 14, 21 and 28, using a Vevo 770 High-Resolution Imaging System with a 37.5-MHz high-frequency linear transducer (VisualSonics Inc., Toronto, ON, Canada) as described previously (Wojciechowski et al. 2010 (link)). Briefly, mice were anesthetized with 3% isoflurane and maintained with 1.5% isoflurane supplemented with 100% O2. A guided M-mode echocardiogram was recorded through the anterior and posterior LV walls at 21 frames/s. Images were obtained at the level of the papillary muscle tips, and measurements were then performed to obtain the LV internal dimension (LVID; in mm), LV posterior wall thickness (LVPW; in mm). LV percent fractional shortening (FS %) and ejection fraction (EF %) were calculated via VisualSonics Measurement Software.
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