Antibiotic susceptibility profile for A. hydrophila Ah17 was determined by the Kirby‐Bauer disk diffusion method (Bauer, Kirby, Sherris, & Turck, 1966 ). The following antibiotics were tested: Amikacin (AK: 30 μg), Amoxicillin (AMC: 30 μg), Ampicillin (AMP: 10 μg), Azithromycin (AZM: 15 μg), Cefixime (CFM: 5 μg), Cefoperazone (CPZ: 75 μg), Cefpodoxime (CPD: 10 μg), Ciprofloxacin (CIP: 5 μg), Chlorompenicol (C: 30 μg), Clarithromycin (CLR: 15 μg), Co‐Trimoxazole (COT: 25 μg), Doxycycline hydrochloride (DO: 30 μg), Fosfomycin (FO: 200 μg), Fusidic acid (FC: 10 μg), Gentamicin (GEN: 10 μg), Imipenem (IPM: 10 μg), Kanamycin (K: 30 μg), Linezolid (LZ: 30 μg), Methicillin (MET: 5 μg), Minocycline (MI: 30 μg), Nalidixic acid (NA: 30 μg), Nitrofurantoin (NIT: 300 μg), Norfloxacin (NX: 10 μg), Penicillin G (P: 10 μg), Pristinomycin (RP: 15 μg), Rifampicin (RIF: 5 μg), Streptomycin (S: 10 μg), Teicoplanin (TEI: 30 μg), Tetracycline (TE: 30 μg), Trimethoprim (TR: 5 μg), Tobramycin (TOB: 10 μg), and Vancomycin (VA: 30 μg) (HiMedia). 200 μl of A. hydrophila Ah17 suspension was swabbed on Mueller‐Hinton agar (MHA) medium (HiMedia) and kept for drying. Antibiotic discs were placed on the MHA medium and incubated at 37°C for 24–48 hr. The diameter of the zone of inhibition was measured, and susceptibility was categorized according to the manufacturer's protocol.
Clarithromycin
Manufactured by HiMedia
Sourced in India
Clarithromycin is a broad-spectrum antibiotic that belongs to the macrolide class of antibiotics. It is commonly used in the treatment of various bacterial infections.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by HiMedia (3 mentions)
Tetracycline, by HiMedia (3 mentions)
Ciprofloxacin, by HiMedia (2 mentions)
Nalidixic acid, by HiMedia (2 mentions)
Amoxicillin, by HiMedia (2 mentions)
Metronidazole, by HiMedia (2 mentions)
Teicoplanin, by HiMedia (1 mentions)
Fosfomycin, by HiMedia (1 mentions)
Methicillin, by HiMedia (1 mentions)
Minocycline, by HiMedia (1 mentions)
Nitrofurantoin, by HiMedia (1 mentions)
Ampicillin, by HiMedia (1 mentions)
Kanamycin, by HiMedia (1 mentions)
Vancomycin, by HiMedia (1 mentions)
Amikacin, by HiMedia (1 mentions)
Norfloxacin, by HiMedia (1 mentions)
Tobramycin, by HiMedia (1 mentions)
Trimethoprim, by HiMedia (1 mentions)
Azithromycin, by HiMedia (1 mentions)
Carbenicillin, by HiMedia (1 mentions)
5 protocols using clarithromycin
1
Antibiotic Susceptibility Profiling of A. hydrophila
2
Antibiotic Susceptibility of H. pylori
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A modified disc diffusion method was used to investigate the susceptibility of H. pylori isolates to clarithromycin (15 mg) and metronidazole (5 mg) (HIMEDIA, Mumbai, India). For this purpose, bacterial suspensions were prepared in the sterile saline (2 mL) equivalent to 3 McFarland standard (∼9.0 × 108 CFU/mL). The suspensions were streaked onto Müller–Hinton agar supplemented with 5% sheep blood (Bahar-Afshan, Tehran, Iran). After 10 min of inoculation, antibiotic discs were placed and incubated in a microaerophilic atmosphere at 37°C for 5–8 days. Susceptibility testing and interpretive criteria were interpreted according to CLSI guideline; inhibition zone for metronidazole was <16 mm and no inhibition zone for clarithromycin.
3
Antimicrobial Susceptibility Testing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antimicrobial susceptibility testing was performed according to the protocols of the CLSI [44 ]. The results were evaluated according to the EUCAST cut-off values [45 ] using antibiotics applicable to the treatment of patients, namely meropenem (10 µg, MEM10C) from Mast Group Ltd., UK, penicillin-G (10 U, SD028-1PK), ampicillin (10 µg, SD002-1PK), amoxycillin (25 µg, SD129-1PK), amoxycillin/clavulanic acid (20/10 µg, AUG30C), carbenicillin (100 µg, SD004-1PK), cefamandole (30 µg, SD200-1PK), erythromycin (15 µg, SD013-1PK), clarithromycin (15 µg, SD192-1PK), streptomycin (10 µg, SD031-1PK), tetracycline (30 µg, SD037-1PK), doxycycline hydrochloride (30 µg, SD012-1PK), chloramphenicol (30 µg, SD006-1PK), nalidixic acid (30 µg, SD021-1PK), ciprofloxacin (5 µg, SD060-1PK), pefloxacin (5 µg, SD070-1PK), and co-trimoxazole (25 µg, SD010-1PK) from HiMedia, India.
4
Antibiotic Susceptibility of Helicobacter pylori
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Susceptibility of H. pylori clinical isolates to clarithromycin (2 μg), metronidazole (5 μg), amoxicillin (10 μg), and tetracycline (30 μg) (HI Media Laboratories, Mumbai, India) was evaluated by the disc diffusion test. For disk diffusion purposes, H. pylori isolates were cultured on Mueller Hinton agar (Merck, Germany) containing 10% defibrillated sheep blood and were incubated for 72 h at 37°C under microaerophilic conditions. Then, a McFarland 3 turbidity standard was prepared by adding colonies into the brain heart infusion (BHI) broth (Merck, Germany). Afterward, it was inoculated into the Mueller Hinton agar, and antibiotic disks were placed. Finally, plates were incubated at 37°C for 3 days under microaerophilic conditions. Subsequently, an agar dilution test was performed based on the guidelines of the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Accordingly, serial dilution was performed by adding inoculum in each glass test tube. The range of final concentrations for each antibiotic were 1–4 μg/mL for tetracycline, 2–64 μg/mL for metronidazole, 0.25–1 μg/mL for amoxicillin, and 0.125–1 μg/mL for clarithromycin. All the powder antibiotics were obtained from Sigma Company, China.
5
Gefitinib-Eudragit RL100 Nanoparticle Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gefitinib and Eudragit® RL100 were obtained as gift samples from Neon Laboratories Ltd. (Mumbai, India) and Dr Reddy’s Laboratories Ltd. (Hyderabad, India), respectively. Polyvinyl alcohol (PVA) with molecular weight 13,000 was obtained from National Chemicals (Gujarat, India). Polyvinylpyrrolidone (PVP) K30 with molecular weight 40,000 was procured from HiMedia® Laboratories Pvt. Ltd. (Mumbai, India). Methanol high-performance liquid chromatography (HPLC) grade and dimethyl sulfoxide (DMSO) were purchased from Finar Ltd. (Ahmedabad, India). Highly pure water (18.2 MΩ cm resistivity, Milli Q) was produced in the laboratory. Dulbecco’s Modified Eagle’s Media (DMEM) and trypsin ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Fetal bovine serum (FBS), sterile phosphate-buffered saline (PBS), 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT), and antibiotic solution (a mixture of penicillin, streptomycin, and clarithromycin) were obtained from HiMedia® Laboratories Pvt. Ltd. All other chemicals were purchased from Merck Ltd. (Mumbai, India).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!