Vitamin d3

Vitamin C (Tianjin Kingyork, H12020392), Vitamin D3 (Beijing Solarbio Science & Technology, V8070), Mouse TNF-alpha ELISA Kit (RayBiotech, P06804), Mouse IL-6 ELISA Kit (RayBiotech, P08505), Mouse IL-1β ELISA Kit (Shanghai Tongwei Biotechnology, TW56961), Mouse insulin ELISA Kit (Shanghai Tongwei Biotechnology, TW085566), Mouse endotoxin (ET) ELISA Kit (Jiangsu Meimian, MM-0369M1), Total Cholestenone Content Assay Kit (Beijing Solarbio Science & Technology, BC 1985), Triglyceride Content Assay Kit (Beijing Solarbio Science & Technology, BC0625), Total SOD activity detection kit (Shanghai Beyotime Biotechnology, S0101S), Lipid oxidation detection kit (Shanghai Beyotime Biotechnology, S0131S), BCA protein concentration assay kit (Shanghai Beyotime Biotechnology, P0010), Oil red O (Sigma, O0625), Anti-FXR1 antibody (Abcam, ab155124), BSEP Monoclonal antibody (Proteintech Group, 67512-1-Ig), Anti-Fas antibody (Abcam, ab271016), Anti-ZO1 tight junction protein antibody (Abcam, ab221547), Anti-Occludin antibody (Abcam, ab222691), Anti-SLC10A2/ASBT antibody (Abcam, ab203205), TRIzol Reagent (Thermo Fisher, 15596026), cDNA synthesis kit (Beijing Tiangen Biotech, KR118), and SuperReal PreMix Plus (SYBR Green) (Beijing Tiangen Biotech, FP205) were used.

DSS (Mr: 36,000–50,000) was purchased from MP Biomedicals (Santa Ana, CA, USA). Vitamin C and Vitamin D₃ were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

β2-GP1 was purchased from Novoprotein (Cat. No. DC432, USA). Incomplete Freund's adjuvant (IFA) (Cat. No. 642862) and complete Freund's adjuvant (CFA) (Cat. No. 642852) were purchased from Solarbio (China). Progesterone was purchased from Solarbio (Cat. No. SP9440). Aspirin enteric-coated tablets were purchased from Bayer HealthCare (Cat. No. J20171021, USA). Vitamin D3 was purchased from Solarbio (Cat. No. V8070). A mouse spleen lymphocyte separation reagent kit was purchased from TBD Sciences (LTS1092PK, China). A mouse β2-GPI ELISA kit (Cat. No. MM-0951 M1), mouse interferon (IFN)-γ ELISA kit (Cat. No. MM-0182 M1), mouse ACA ELISA kit (Cat. No. MM-0583 M1), mouse interleukin (IL)-6 ELISA kit (Cat. No. MM-0163 M1), and mouse CXCL6 ELISA kit (Cat. No. MM-0950 M1) were all purchased from Meimian Biotechnology (China). CD4-FITC (Cat. No. 100405), CD25-PE/CY7 (Cat. No. 102015), CD49b-PE (Cat. No. 108907), FOXP3-Alexa Fluor 647 (Cat. No. 320013), CD45-Percp/CY5.5 (Cat. No. 103131), fixation buffer (Cat. No. 420801), and intracellular staining permeabilization wash buffer (Cat. No. 421002) were all purchased from BioLegend (USA).