129S2 mice were obtained from Charles River and C57BL/6.J (B6, CD45.2) and B6.SJL-Ptprca.Pep3b/BoyJ (CD45.1) were obtained from Taconic. Wnt5a+/− (Yamaguchi et al., 1999 (link)) mice obtained from The Jackson Laboratory were backcrossed to the (129S2 x C57BL/6.J)F1 (129B6, CD45.2) background for at least six generations. Age- and gender-matched Wnt5a+/+ (WT) littermates were used as controls in all experiments. In transplantation experiments, first-generation crosses of 129S2 and B6.SJL-Ptprca Pepcb/BoyJ (129Ly5.1, CD45.1xCD45.2) were used as recipients in intrinsic transplantation assays and as donors in extrinsic transplantations. All animal experiments were approved by the section Consumer Protection, Veterinary Services, and Food Hygiene of the Government of Upper Bavaria (Regierung Oberbayern, Munich, Germany). All animals were housed for at least a week before experimental use in microisolators under specific pathogen–free conditions, according to the Federation of Laboratory Animal Science Associations and institutional recommendations.
C57bl 6j b6
Manufactured by Charles River Laboratories
Sourced in United States, Japan
C57BL/6J (B6) is a widely used inbred mouse strain. It is one of the most commonly used laboratory mouse strains for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
129s2 mice, by Charles River Laboratories (1 mentions)
B6 sjl ptprca pep3b boyj, by Charles River Laboratories (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Anti cd11c magnetic microbeads, by Miltenyi Biotec (1 mentions)
Icr mice, by Charles River Laboratories (1 mentions)
B6 c h2 ab1bm12 khegj bm12, by Jackson ImmunoResearch (1 mentions)
12 protocols using c57bl 6j b6
1
Wnt5a Heterozygous Mice Transplantation
2
Genetic Regulation of Taste Perception in Mice
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The subjects were adult male C57BL/6J (B6) (Charles River; n = 58) mice, T1R3 green fluorescent protein (GFP) [26 (link)] (n = 3) mice, and T1R3-KO (n = 14) [27 (link)] mice. The T1R3-GFP mice and T1R3-KO mice were originally derived from the C57BL/6J strain at Mount Sinai Medical School via homologous recombination in C57BL/6 embryonic stem cells and were maintained in this genetic background (age: 8–16 weeks; weight: 20–32 g). We used male mice to avoid the effects of sex differences because the ADM gene is reported to be directly activated by estrogen receptors in mice [28 (link)].
3
Isolation and Study of Murine Immune Cells
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Female C57BL/6J (B6) and OT-2 TCR transgenic mice were purchased from Charles River and housed at the animal facility of Istituto Superiore di Sanità of Rome (ISS), according to institutional guidelines (DL116/92 and 26/2014). Female OT-1 TCR transgenic mice were kindly provided by Dr. M. R. Castrucci (ISS). The OT-1 transgenic TCR recognizes the Kb-restricted OVA 257–264 peptide (35 (link)), while the OT-2 transgenic TCR recognizes the I-Ab-restricted OVA 323-339 peptide (36 (link)). CX3cr1gfp/+ and CX3cr1gfp/gfp B6 mice were purchased from JAX Mice and Services (Bar Harbor, ME, USA) (37 (link)). Mice were sacrificed at 5–16 weeks of age and spleen, peripheral, and mesenteric LNs and BM obtained as we previously described (38 (link), 39 (link)). In some experiments, CD11c+ cells were enriched from either spleen or BM with anti-CD11c magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany).
4
Diabetic Mouse Model: Compound 49b Treatment
5
Forced Running Endurance in Mice
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All animal procedures were approved by the Lithuanian State Food and Veterinary Service (Ref. # 0230). Breeding nuclei of the C57BL/6J (B6) and C57BL/6J-Chr 10A/J/NaJ (B6.A10) strains were purchased from Charles River and the Jackson Laboratory, respectively. Samples of the experimental animals were bred locally.
Mice were housed in standard cages, one to three same sex animals per cage, ambient temperature 20–21°C and 40–60 % humidity, with 12-h light/ 12-h dark cycle. Animals were fed standard chow diet (LabDiet 5001) and received tap water ad libitum. The forced running experimental procedure on 16 B6 (8 females and 8 males) and 21 B6.A10 (7 and 14, respectively) was initiated at 12–13 week of age. Voluntary wheel running was measured in separate group of 10 B6 and 10 B6.A10 males at 15–16 week of age. The ex vivo testing was carried out on a separate group of 6 B6 and 6 B6.A10 males of 18–19 week of age.
Mice were housed in standard cages, one to three same sex animals per cage, ambient temperature 20–21°C and 40–60 % humidity, with 12-h light/ 12-h dark cycle. Animals were fed standard chow diet (LabDiet 5001) and received tap water ad libitum. The forced running experimental procedure on 16 B6 (8 females and 8 males) and 21 B6.A10 (7 and 14, respectively) was initiated at 12–13 week of age. Voluntary wheel running was measured in separate group of 10 B6 and 10 B6.A10 males at 15–16 week of age. The ex vivo testing was carried out on a separate group of 6 B6 and 6 B6.A10 males of 18–19 week of age.
6
Transgenic Mice Breeding and Housing
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Timed-pregnant female wild-type C57BL/6J (B6) (Charles River, USA) and transgenic Gnrh-Gfp62 (link) were group-housed under specific pathogen-free conditions in a temperature-controlled room (21-22°C) with a 12-h light/dark cycle and ad libitum access to food and water. Standard diet (9.5 mm Pelleted RM3, Special Diets Services, France) was given to all mice during breeding, lactation and growth of young stock. Nutritional profile of the standard diet RM3 is the following: Protein 22.45%, Fat 4.2%, Fiber 4.42%, Ash 8%, Moisture 10%, Nitrogen free extract 50.4%; Calories: 3.6 kcal/gr.
Mice were randomly assigned to groups at the time of purchase or weaning to minimize any potential bias. No data sets were excluded from analyses.
C57BLl/6J Gnrh-Gfp transgenic mice have been previously characterized62 (link). Animal studies were approved by the Institutional Ethics Committees of Care and Use of Experimental Animals of the University of Lille (France; Ethical protocol number: APAFIS#2617-2015110517317420 v5). All experiments were performed in accordance with the guidelines for animal use specified by the European Council Directive of 22 September 2010 (2010/63/EU). The sample size, sex and age of the animals used is specified in the text and/or figure legends.
Mice were randomly assigned to groups at the time of purchase or weaning to minimize any potential bias. No data sets were excluded from analyses.
C57BLl/6J Gnrh-Gfp transgenic mice have been previously characterized62 (link). Animal studies were approved by the Institutional Ethics Committees of Care and Use of Experimental Animals of the University of Lille (France; Ethical protocol number: APAFIS#2617-2015110517317420 v5). All experiments were performed in accordance with the guidelines for animal use specified by the European Council Directive of 22 September 2010 (2010/63/EU). The sample size, sex and age of the animals used is specified in the text and/or figure legends.
7
Maintaining B6D2F1, B6, and ICR Mice
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B6D2F1 mice were purchased from CLEA Japan (Kawasaki, Japan). C57BL/6J (B6) and ICR mice were purchased from Charles River Japan (Yokohama, Japan). The mice were maintained on a 12 h light/dark cycle (lights on from 08:00 h to 20:00 h) and were provided with food and water ad libitum. All animal experiments were approved by the Animal Care and Experimentation Committee of Gunma University (No. 17-030), and were carried out in accordance with the approved guidelines.
8
Inbred Mouse Housing and Husbandry
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C57BL/6 J (B6) and Jcl:CD1 (ICR) inbred strain were purchased from Charles River Laboratories (Yokohama, Japan) and CLEA Japan (Tokyo, Japan). All mice were housed in plastic cages under pathogen-free conditions in a room maintained at 23.5 °C ± 2.5 °C and 52.5% ± 12.5% relative humidity under a 14-h light:10-h dark cycle. Mice had free access to commercial chow (MF; Oriental Yeast, Tokyo, Japan) and filtered water. Animal experiments were carried out in a humane manner with approval from the Institutional Animal Experiment Committee of the University of Tsukuba in accordance with the Regulations for Animal Experiments of the University of Tsukuba and Fundamental Guidelines for Proper Conduct of Animal Experiments and Related Activities in Academic Research Institutions under the jurisdiction of the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
9
Genetically Engineered Mouse Models
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Female mice from the C57BL/6 J (B6) and C3H/HeJ (C3H) inbred strains were purchased from Charles River Laboratories and maintained for at least 2 weeks prior to use in experiments to allow them to acclimate to the environmental conditions of the vivarium. Congenic B6.C3HDyscalc1 were generated as reported previously13 (link),14 (link). Abcc6−/− mice were generated on the 129/Ola background and backcrossed onto a C57BL/6 J background at least 10 times28 (link).
10
Diabetic Mouse Model: Compound 49b Treatment
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All mouse experiments were approved by the Institutional Animal Care and Use Committee at Wayne State University (Protocol# 11-08-14) and adhere to the Animal Policy of the Association for Research in Vision and Ophthalmology. Eight week old male C57BL/6J (B6) wild type mice were purchased from Charles River Laboratories. Diabetes was induced by injecting 60mg/kg of streptozotocin dissolved in citrate buffer for 5 consecutive days. Control mice received citrate buffer only. Glucose measurements were done twice weekly, with glucose levels > 250mg/dL considered diabetic. Mice were not fasted before blood glucose measurements. Glucose measurements were taken on blood samples obtained from the tail vein using a hand-held measurement device. Table 1 provides body weights and glucose levels of all mice.
After 2 months of diabetes, 6 control and 6 diabetic mice received 1mM Compound 49b eye drops for 14 days (4 uL onto each eye). Control mice received topical PBS. Mice were randomly placed into the treatment or no treatment groups without blinding by the investigators. After 14 days of Compound 49b treatment, all mice were sacrificed for further analysis as described below.
After 2 months of diabetes, 6 control and 6 diabetic mice received 1mM Compound 49b eye drops for 14 days (4 uL onto each eye). Control mice received topical PBS. Mice were randomly placed into the treatment or no treatment groups without blinding by the investigators. After 14 days of Compound 49b treatment, all mice were sacrificed for further analysis as described below.
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