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Tenofovir disoproxil fumarate

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Tenofovir disoproxil fumarate is a chemical compound used in the production of laboratory equipment. It is a salt of the antiviral drug tenofovir, which is used to treat HIV and hepatitis B infections. The compound serves as a key ingredient in the manufacturing process for certain lab equipment and instrumentation.

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Lab products found in correlation

Emtricitabine, by Gilead Sciences (7 mentions) Dolutegravir, by Gilead Sciences (4 mentions) Abacavir, by Gilead Sciences (3 mentions) Lamivudine, by Gilead Sciences (3 mentions) Tenofovir alafenamide, by Gilead Sciences (3 mentions) Raltegravir, by Gilead Sciences (3 mentions) Elvitegravir, by Gilead Sciences (2 mentions) Bms 626529, by Apexbio (1 mentions) Thapsigargin, by Merck Group (1 mentions) Ritonavir, by Abbott (1 mentions) Nevirapine, by Boehringer Ingelheim (1 mentions) Efavirenz, by Bristol-Myers Squibb (1 mentions) Maraviroc, by Pfizer (1 mentions) Lopinavir, by Abbott (1 mentions) Atazanavir, by Bristol-Myers Squibb (1 mentions) Maraviroc, by Selleck Chemicals (1 mentions) Raltegravir ral, by Merck Group (1 mentions)

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Tenofovir (15 citations) Tenofovir disoproxil fumarate (TDF; (3 citations)

10 protocols using tenofovir disoproxil fumarate

1

Tenofovir and Emtricitabine Assay Protocol

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Tenofovir disoproxil fumarate (TDF) and emtricitabine (FTC) were kindly provided by Gilead Sciences, Inc. (Foster City, CA), under a material transfer agreement (MTA) dated August 8, 2011. Tenofovir, [adenine-13C5] (TFV-13C5) was obtained from Moravek Biochemicals, Inc. (Brea, CA) and maraviroc-D6 (MVC-D6) was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX).
Srinivasan P., Moss J.A., Gunawardana M., Churchman S.A., Yang F., Dinh C.T., Mitchell J.M., Zhang J., Fanter R., Miller C.S., Butkyavichene I., McNicholl J.M., Smith T.J., Baum M.M, & Smith J.M. (2016). Topical Delivery of Tenofovir Disoproxil Fumarate and Emtricitabine from Pod-Intravaginal Rings Protects Macaques from Multiple SHIV Exposures. PLoS ONE, 11(6), e0157061.
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2

Tenofovir Disoproxil Fumarate Formulation Development

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Tenofovir disoproxil fumarate (TDF) was kindly provided by Gilead Sciences, Inc. (Foster City, CA). Liquid silicone resin (LSR, MED-4940 and MED-4840) and silicone adhesive (MED3-4213) was obtained from Nusil, Inc. (Carpenteria, CA). Polyvinyl alcohol, USP (PVA, viscosity = 23.6 mPa s, 85-89% hydrolyzed) was obtained from Spectrum Chemical (Gardena, CA). D,L-Polylactic acid (PLA, Resomer R 202 S) was obtained from Evonik Industries AG (Darmstadt, Germany). All other chemicals were NF grade or equivalent and used as received.
Baum M.M., Butkyavichene I., Churchman S.A., Lopez G., Miller C.S., Smith T.J, & Moss J.A. (2015). An intravaginal ring for the sustained delivery of tenofovir disoproxil fumarate. International journal of pharmaceutics, 495(1), 579-587.
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3

Robust HIV Infection Assay Protocol

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NBD-14168 and NBD-14189 were reported earlier [2 (link)], and BMS-626529 was purchased from Apexbio, Boston, MA. Tenofovir disoproxil fumarate (TDF) and emtricitabine (FTC) were obtained from Gilead Sciences. HIV molecular clone pNL4-3 (CXCR4) from M. Martin [39 (link)] was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH. To prepare the infectious supernatant HEK 293T cells were transfected using lipofectamine 2000. To calculate the stock virus titers, we infected human peripheral blood mononuclear cells (PBMCs) stimulated with phytohaemagglutinin (PHA) and assessed the supernatant p24 by endpoint dilution by enzyme-linked immunosorbent assay (ELISA) after 7 days. The 50% tissue culture infective doses (TCID50) were calculated using the Reed-Muench method.
Stoddart C.A., Curreli F., Horrigan S., Altieri A., Kurkin A.V, & Debnath A.K. (2022). Comparative Pharmacokinetics of a Dual Inhibitor of HIV-1, NBD-14189, in Rats and Dogs with a Proof-of-Concept Evaluation of Antiviral Potency in SCID-hu Mouse Model. Viruses, 14(10), 2268.
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4

Antivirals and HIV Plasmid Protocol

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The antivirals Emtricitabine (FTC), Tenofovir Disoproxil Fumarate (TDF), Raltegravir (RAL), abacavir (ABC), dolutegravir (DOL), lamivudine (3TC), tenofovir alafenamide (TAF) were a gift from Gilead Sciences or were obtained from pharmacy. HIV p89.6 plasmid DNA was obtained from the NIH AIDS reagent Program. Murine chow diet with antibiotic was purchased from Envigo Teklad Diets. All other materials were purchased from commercially available sources and have been previously described in detail [15 (link)]. All lipids were purchased from Cayman Chemicals (Ann Arbor, MI, USA) and are described in detail in the Supplemental material.
Daskou M., Sharma M., Mu W., Heymans R., Ritou E., Rezek V., Hamid P., Kossyvakis A., Sen Roy S., Grijalva V., Chattopadhyay A., Papesh J., Meriwether D., Kitchen S.G., Fogelman A.M., Reddy S.T, & Kelesidis T. (2021). ApoA-I mimetics favorably impact cyclooxygenase 2 and bioactive lipids that may contribute to cardiometabolic syndrome in chronic treated HIV. Metabolism: clinical and experimental, 124, 154888.
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5

Antiretroviral Drug Effects on Cell Viability

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The following chemicals/drugs were used: zidovudine (GlaxoSmithKline), stavudine (Bristol-Myers Squibb), tenofovir disoproxil fumarate (Gilead Sciences), nevirapine (Boehringer Ingelheim), efavirenz (Bristol-Myers Squibb), rilpivirine (Janssen Pharmaceuticals, Inc.), ritonavir (Abbott Laboratories), lopinavir (Abbott Laboratories), nelfinavir (Agouron), atazanavir (Bristol-Myers Squibb), raltegravir (sc-364600; Santa Cruz), elvitegravir (Gilead Sciences), maraviroc (Pfizer), tunicamycin (T7765; Sigma-Aldrich Biotechnology), thapsigargin (T9033; Sigma-Aldrich), 4-phenylbutyric acid ([PBA] P21005; Sigma-Aldrich), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid ([Trolox] 648471; Calbiochem), and rosiglitazone (ALX-350-125; Alexis Biochemicals). The reagents for cell culture were from Sigma-Aldrich, whereas media and FBS were from Life Technologies. For initial assessments of the effects of antiretroviral drugs prior to concentration-response analyses, the drugs were used at the highest concentrations that did not cause cytotoxicity in specific cell systems. The drugs were dissolved using dimethyl sulfoxide (DMSO) as the vehicle. Controls included amounts of DMSO (≤0.1% DMSO of total cell medium volume) equal to those used in drug-treated cells.
Moure R., Domingo P., Villarroya J., Gasa L., Gallego-Escuredo J.M., Quesada-López T., Morón-Ros S., Maroto A.F., Mateo G.M., Domingo J.C., Villarroya F, & Giralt M. (2018). Reciprocal Effects of Antiretroviral Drugs Used To Treat HIV Infection on the Fibroblast Growth Factor 21/β-Klotho System. Antimicrobial Agents and Chemotherapy, 62(6), e00029-18.
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6

Antivirals and HIV Plasmid Protocol

Cited 1 time
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The antivirals Emtricitabine (FTC), Tenofovir Disoproxil Fumarate (TDF), Raltegravir (RAL), abacavir (ABC), dolutegravir (DOL), lamivudine (3TC), tenofovir alafenamide (TAF) were a gift from Gilead Sciences or were obtained from pharmacy. HIV p89.6 plasmid DNA was obtained from the NIH AIDS reagent Program. Murine chow diet with antibiotic was purchased from Envigo Teklad Diets. All other materials were purchased from commercially available sources and have been previously described in detail [15 (link)]. All lipids were purchased from Cayman Chemicals (Ann Arbor, MI, USA) and are described in detail in the Supplemental material.
Daskou M., Sharma M., Mu W., Heymans R., Ritou E., Rezek V., Hamid P., Kossyvakis A., Sen Roy S., Grijalva V., Chattopadhyay A., Papesh J., Meriwether D., Kitchen S.G., Fogelman A.M., Reddy S.T, & Kelesidis T. (2021). ApoA-I mimetics favorably impact cyclooxygenase 2 and bioactive lipids that may contribute to cardiometabolic syndrome in chronic treated HIV. Metabolism: clinical and experimental, 124, 154888.
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7

HIV Drug Combination Evaluation

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Tenofovir disoproxil fumarate (TDF), emtricitabine (FTC), and elvitegravir (EVG) kindly were provided by Gilead Sciences, Inc (Foster City, CA), or purchased from Selleck Chemicals LLC (Houston, TX). Maraviroc (MVC) was purchased from Selleck Chemicals LLC. The peptide C5A (purity ≥ 80%) was obtained from GenScript USA, Inc (Piscataway, NJ). All other reagents were obtained from Sigma-Aldrich (St. Louis, MO), unless otherwise noted.
Gallay P.A., Ramirez C.M, & Baum M.M. (2023). Acute antagonism in three-drug combinations for vaginal HIV prevention in humanized mice. Scientific Reports, 13, 4594.
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8

Transgenic Tomato-Derived Antiviral Compounds

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A concentrate of control transgenic tomatoes (EV) or a concentrate of transgenic tomatoes expressing the 6F peptide (Tg6F) were prepared and added to the diets at 0.06% by weight as described previously [11 (link)]. 4F and 6F peptides were synthesized as previously [14 (link)]. The antivirals Emtricitabine (FTC), Tenofovir Disoproxil Fumarate (TDF), Raltegravir (RAL), abacavir (ABC), dolutegravir (DOL), lamivudine (3TC), tenofovir alafenamide (TAF), FTC were either a generous gift from Gilead Sciences or were obtained from pharmacy. HIV p89.6 plasmid DNA was obtained from the NIH AIDS reagent Program. Murine chow diet with antibiotic was purchased from Envigo Teklad Diets. All other materials were purchased from commercially available sources and have been previously described in detail [24 (link)].
Daskou M., Mu W., Sharma M., Vasilopoulos H., Heymans R., Ritou E., Rezek V., Hamid P., Kossyvakis A., Sen Roy S., Grijalva V., Chattopadhyay A., Kitchen S.G., Fogelman A.M., Reddy S.T, & Kelesidis T. (2022). ApoA-I mimetics reduce systemic and gut inflammation in chronic treated HIV. PLoS Pathogens, 18(1), e1010160.
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9

Rhesus Macaques SIV Infection and Antiretroviral Therapy

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Animal work was approved by the Institutional Care & Use Committees of Bioqual and the NIH and determined to be in accordance with the guidelines outlined by the Animal Welfare Act and Regulation (USDA) and the Guide for the Care & Use of Laboratory Animals, 8th Edition (NIH).
Cohort 15–09: The details of this study have been described elsewhere20 ,93 (link). Near-full-length sequencing data collected from SIV-infected Rhesus macaques (n=7, Bender, et al.20 ; or n=16, Liu, et al.93 (link)) were used for assay development and verification of assay targets, as detailed in the “HPDA sequence validation” subsection below.
Cohort 18–02: 10 outbred Indian-origin rhesus macaques (Macaca mulatta) were housed at Bioqual Inc. in Rockville, MD. Animals were infected via repetitive intrarectal challenge with the SIVmac251 swarm57 (link) until infection was confirmed by detection of SIV RNA in the plasma via qPCR. After ~48 weeks, the animals were placed on a daily regimen of tenofovir disoproxil fumarate, emtricitabine, and dolutegravir (TDF/FTC/DTG, Gilead Sciences, Inc.58 (link). The animals were MHC class I genotyped as previously described96 (link). MHC genotypes, ages and sexes of the animals in this cohort are detailed below.
Fray E.J., Wu F., Simonetti F.R., Zitzmann C., Sambaturu N., Molina-Paris C., Bender A.M., Liu P.T., Ventura J.D., Wiseman R.W., O’Connor D.H., Geleziunas R., Leitner T., Ribeiro R.M., Perelson A.S., Barouch D.H., Siliciano J.D, & Siliciano R.F. (2023). Antiretroviral therapy reveals triphasic decay of intact SIV genomes and persistence of ancestral variants. Cell host & microbe, 31(3), 356-372.e5.
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10

HIV-1 Infection and ARV Treatment Protocol

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HIV-1JR-CSF stocks were prepared as previously described[4 (link)]. Intraperitoneal infections were one-time, 100μl injections. Liquid formulations of tenofovir disoproxil fumarate (TDF), emtricitabine (FTC) and dolutegravir (DTG) were provided by Gilead Sciences (Foster City, CA) and administered daily by subcutaneous injection. Tablets of TDF, FTC and raltegravir (RAL; Merck & Co., Kenilworth, NJ) were crushed and formulated with TestDiet 5B1Q feed as previously described[10 (link)]. Briefly, powdered TDF, FTC and RAL were incorporated into TestDiet 5B1Q irradiated feed pellets at a final concentration of 720 mg/kg TFV, 520 mg/kg FTC, and 4800 mg/kg RAL.
LAVENDER K.J., PACE C., SUTTER K., MESSER R.J., POUNCEY D.L., CUMMINS N.W., NATESAMPILLAI S., ZHENG J., GOLDSMITH J., WIDERA M., VAN DIS E.S., PHILLIPS K., RACE B., DITTMER U., KUKOLJ G, & HASENKRUG K.J. (2018). An advanced BLT-humanized mouse model for extended HIV-1 cure studies. AIDS (London, England), 32(1), 1-10.
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