Protein extraction was carried out by lysing cells in PBS buffer containing 1% Triton X-100 with added protease and phosphatase inhibitors (Roche). Protein content was determined using Bradford assay (Pierce). Approximately 25 to 70 μg of total protein was resolved on 8, 10 or 12% SDS-PAGE gel before transferring onto PVDF membrane (Millipore) for detection using ECL reagent (Pierce). The following antibodies were used: anti-phospho-JNK, anti-JNK, anti-phospho-MKK4, anti-MKK4, anti-FTH1 (Cell Signaling Technology), anti-TRX (Santa Cruz), anti-LC3B (Abgent), anti-SOD2 (Abcam), anti-actin and HRP-conjugated anti-rabbit and anti-mouse (Sigma).
Anti lc3b
Manufactured by Abcepta
Sourced in United States
Anti-LC3B is a primary antibody that recognizes the microtubule-associated protein Light Chain 3 B (LC3B). LC3B is a widely used marker for the detection and quantification of autophagosomes, which are key components of the cellular autophagy process.
Automatically generated - may contain errors
Lab products found in correlation
Protease and phosphatase inhibitor, by Roche (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti fth1, by Cell Signaling Technology (1 mentions)
Anti mkk4, by Cell Signaling Technology (1 mentions)
Anti trx, by Santa Cruz Biotechnology (1 mentions)
Anti sod2, by Abcam (1 mentions)
Anti phospho jnk, by Cell Signaling Technology (1 mentions)
Anti jnk, by Cell Signaling Technology (1 mentions)
Anti phospho mkk4, by Cell Signaling Technology (1 mentions)
3 methyladenine 3 ma, by Merck Group (1 mentions)
Chloroquine cq, by Merck Group (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Anti β actin, by Boster Bio (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti mtor, by Cell Signaling Technology (1 mentions)
Ici 182 780, by Merck Group (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Caspase 3, by Proteintech (1 mentions)
2 protocols using anti lc3b
1
Protein Extraction and Western Blot
2
Modulation of ER-α36 Signaling and Autophagy
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Tamoxifen, estrogen receptor antagonist ICI182 780, subtype selective ERα agonist PPT (4,4′,4″‐(4‐propyl‐1H‐pyrazole‐1,3,5‐triyl) trisphenol), autophagic inhibitor 3‐MA (3‐methyladenine) and CQ (chloroquine) were purchased from Sigma (St Louis, MO, USA). Rabbit polyclonal anti‐ER‐α36 antibody, ER‐α36 expression vector and ER‐α36 specific modulator (IC162) were from Dr ZY Wang (Creighton University Medical School). Rabbit monoclonal anti‐p‐Akt, anti‐Akt, anti‐p‐mammalian target of rapamycin (mTOR), anti‐mTOR, anti‐Bcl‐2 and mouse monoclonal anti‐glial fibrillary acidic protein (anti‐GFAP) were purchased from Cell Signaling Technology (Boston, MA, USA). Rabbit monoclonal anti‐LC3B was purchased from Abgent (San Diego, CA, USA), anti‐β‐actin was purchased from Boster (Wuhan, China), and rabbit polyclonal anti‐GAPDH, P53, and caspase3 were from Proteintech (Wuhan, China).
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