Bax antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Bax antibody is a laboratory tool used for the detection and analysis of the Bax protein. Bax is a pro-apoptotic member of the Bcl-2 protein family, which plays a crucial role in the regulation of programmed cell death or apoptosis. The Bax antibody can be used in various immunological techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to identify and quantify the Bax protein in biological samples.

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Lab products found in correlation

Bcl 2 antibody, by Santa Cruz Biotechnology (6 mentions) β actin antibody, by Santa Cruz Biotechnology (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Caspase 3, by Cell Signaling Technology (3 mentions) Cleaved caspase 3, by Cell Signaling Technology (3 mentions) β actin, by Merck Group (3 mentions) Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Ripa lysis buffer, by Beyotime (2 mentions) Phospho extracellular signal regulated kinase erk 1 2, by Cell Signaling Technology (2 mentions) Erk1 2, by Cell Signaling Technology (2 mentions) Bcl 2, by Santa Cruz Biotechnology (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Rhodamine 123, by Beyotime (1 mentions) Sds page gel quick preparation kit, by Beyotime (1 mentions) Trizol, by Beyotime (1 mentions) Supersignal west pico chemiluminescent substrate trial kit, by Thermo Fisher Scientific (1 mentions) Caspase 3 antibody, by Santa Cruz Biotechnology (1 mentions) Sybr premix ex taq 2, by Takara Bio (1 mentions) Annexin 5 fitc apoptosis detection kit, by Beyotime (1 mentions)

Close spelling variants of this product

Bax 6A7 antibody (2 citations) Goat anti-rat Bax polyclonal antibody (2 citations) Rabbit Bax antibody (4 citations) Anti-BAX monoclonal antibody (2 citations) Bax mouse monoclonal antibody (3 citations) Mouse Bax antibody (4 citations) Antibody against Bax (2 citations) Anti-Bax antibody (sc-20067, (2 citations) BAX N20 antibody (4 citations) Rabbit anti-Bax antibody (3 citations)

18 protocols using bax antibody

Peptide Regulation of Hair Follicle Cell Apoptosis

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Example 7

Human hair follicle dermal papilla cells were seeded at a density of 4×10⁵cells/well on 6-well plate, followed by incubation overnight. After changing into serum-free medium, the cells were treated with the peptides, followed by incubation for 24 hours, and then the wells were harvested to prepare cell lysate. Western blotting was performed using Bcl-2 and Bax antibodies (Santa Cruz Biotechnology, USA) to compare protein expression patterns. The results are shown in FIGS. 7a and 7b.

As can be confirmed from FIGS. 7a and 7b, the expression of the anti-apoptotic protein Bcl-2 was increased and the expression of the apoptosis-related protein Bax was decreased in human hair follicle dermal papilla cells by the treatment with the peptide composed of the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2.

US10508140B2. Peptide having hair growth-promoting activity and/or melanin generation-promoting activity, and use thereof (2019-12-17). CAREGEN CO., LTD. [KR]. Inventors: Yong Ji Chung [KR], Eun Mi Kim [KR], Eung-Ji Lee [KR].

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Peptide Regulation of Bcl-2 and Bax in Dermal Papilla Cells

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Example 4

As can be confirmed from FIGS. 4a and 4b, the expression of the anti-apoptotic protein Bcl-2 was increased and the expression of the apoptosis-related protein Bax was decreased in human hair follicle dermal papilla cells by the treatment with the peptide composed of the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2.

US10344061B2. Peptide exhibiting hair growth promoting activity and/or melanin production promoting activity and use thereof (2019-07-09). CAREGEN CO., LTD. [KR]. Inventors: Yong Ji Chung [KR], Eun Mi Kim [KR], Eung Ji Lee [KR].

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Edaravone Injection Cytotoxicity Evaluation

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Edaravone injection was purchased from Jiangsu Simcere Pharmaceutical Co., Ltd. (molecular weight: 174.20). Fetal bovine serum (FBS), phosphate buffered solution (PBS), Dulbecco's modified Eagle's medium (DMEM), and trypsin (with phenol red) were purchased from Hyclone (GE Healthcare). MTT cell proliferation and cytotoxicity assay kit, Annexin V-FITC apoptosis detection kit, reactive oxygen species (ROS) assay kit, Rhodamine 123, Trizol, RIPA lysis buffer, enhanced BCA protein assay kit, and SDS-PAGE gel quick preparation kit were purchased from Beyotime. Revert aid first strand cDNA synthesis kit and Super Signal West Pico Chemiluminescent Substrate Trial kit were purchased from Thermo Fisher Scientific Inc. SYBR® Premix Ex Taq™ II was purchased from Takara Bio Inc. Bcl-2 antibodies, Bax antibodies, caspase-3 antibodies, and β-actin antibodies were purchased from Santa Cruz Biotech.

Xu X., Zhang H., Wang K., Tu T, & Jiang Y. (2017). Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes. Biochemistry Research International, 2017, 5839762.

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Bax Protein Localization by IHC

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Immunohistochemistry was used to localize the Bax antigen. Bax antibodies (Santa Cruz Biotechnology Inc.) were put at 4°C overnight. The appropriate horseradish peroxidase conjugated secondary antibodies were added and the samples were incubated for 1 h at room temperature (1:6,000 dilution for Bax) as described previously (Buytaert et al. 2006 (link)).

Hussien N.I, & Mousa A.M. (2016). Could nitric oxide be a mediator of action of oxytocin on myocardial injury in rats? (Biochemical, histological and immunohistochemical study). General physiology and biophysics, 35(3).

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PLGA-based Nanoformulations for Cancer Therapy

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PLGA 50:50 (molecular weight 40–75 kDa), polyvinyl alcohol (molecular weight 30 kDa), 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT), TF, EGCG, CDDP, propidium iodide, and β-actin (clone AC-74) were purchased from Sigma-Aldrich (St Louis, MO, USA). Caspase-3, caspase-9, cytochrome C, p-NF-κB, p-IκBα, p53, and Bcl-2 antibodies were sourced from Cell Signaling Technology (Beverly, MA, USA) while Bax antibody was obtained from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA). The rabbit anti-mouse and goat anti-rabbit horseradish peroxidase-conjugated secondary antibodies were obtained from Bangalore Genei (Bangalore, India). The polyvinylidene fluoride membrane was obtained from Millipore (Bedford, MA, USA). Caspase-3 inhibitor z-DEVD-fmk was purchased from Calbiochem (Boston, MA, USA). Fetal bovine serum, Dulbecco’s Modified Eagle’s Medium, and Roswell Park Memorial Institute medium were supplied by Invitrogen (Invitrogen, Carlsbad, CA, USA) and the antibiotics by Gibco (Lifetech, Karlsruche, Germany). 2′,7′-dichlorofluorescein diacetate (DCF-DA) and rhodamine 123 from BD Pharmingen (San Diego, CA, USA) were used. Other chemicals used were of analytical grade and sourced locally.

Singh M., Bhatnagar P., Mishra S., Kumar P., Shukla Y, & Gupta K.C. (2015). PLGA-encapsulated tea polyphenols enhance the chemotherapeutic efficacy of cisplatin against human cancer cells and mice bearing Ehrlich ascites carcinoma. International Journal of Nanomedicine, 10, 6789-6809.

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Protein Expression Analysis in Prefrontal Cortex

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Protein concentration in the PFC was determined as reported previously. Equal quantities of protein were loaded onto a 10–15% gradient polyacrylamide gel, electrophoretically transferred to a polyvinylidene difluoride membrane and probed with the following primary antibodies: Bcl-2 antibody (1:1000, Santa Cruz Biotechnology, CA, USA), Bax antibody (1:1000, Santa Cruz Biotechnology), Shh (1:1000, Proteintech.), Gli-1 (1:1000, Santa Cruz Biotechnology), Ptch (1:1000, Abcam, Cambridge, MA, USA), caspase-3 (1:500, Cell Signaling Tech. MA, USA), cleaved caspase-3 (1:1000, Cell Signaling), Phospho-extracellular signal-regulated kinase (ERK)1/2 (1:2000, Cell Signaling), ERK1/2 (1:2000; Cell Signaling), Nrf2 (1:1000, Proteintech.), HO-1 (1:2000, Proteintech.). β-actin (1:2000; Sigma-Aldrich) was used as an internal loading control.

Hu Q., Li T., Wang L., Xie Y., Liu S., Bai X., Zhang T., Bo S., Xin D., Xue H., Li G, & Wang Z. (2017). Neuroprotective Effects of a Smoothened Receptor Agonist against Early Brain Injury after Experimental Subarachnoid Hemorrhage in Rats. Frontiers in Cellular Neuroscience, 10, 306.

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Cell Viability and Apoptosis Assays

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ICG, Cell Counting Kit-8 (CCK8), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD), calcein-AM/propidium iodide (PI)/fluorescein isothiocyanate (FITC), phosphate-buffered saline (PBS), and BCA protein assay kit were obtained from Beyotime (China). Penicillin−streptomycin, fetal bovine serum (FBS), and high-glucose Dulbecco’s modified Eagle medium (DMEM) were acquired from ThermoFisher (Waltham, MA, USA). RIPA lysis buffer was obtained from Beyotime Biotechnology (China). Antibody against gp100 was provided by BD Biosciences (USA). Antibody against Na⁺/K⁺-ATPase was purchased from GenScript (USA). Histone H3(H3) antibody, cytochrome c oxidase subunit IV(COXIV) antibody and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody were purchased from Proteintech Company (China). β-actin antibody was obtained from Santa Cruz Biotechnology (USA). Antibodies against caspase-9/cleaved caspase-9 were purchased from Thermo Fisher Scientific (USA). BAX antibody was obtained from Santa Cruz Biotechnology (USA). The 220-nm polycarbonate membranes were provided by Merck Millipore (Germany). B16, L929, and 4T1 cell lines were generous gifts from the National Engineering Laboratory of AIDS Vaccines of Jilin University,16 (link) and the use of the cell lines was approved by the institutional research ethics committee of China-Japan Union Hospital of Jilin University.

Wang Y., Zhao Z., Liu C., Hao M., Kong C., Zhao X., Gao Y., Zhang Y., Cui W., Zhang C, & Jiang J. (2022). B16 Membrane-Coated Vesicles for Combined Photodynamic Therapy and Immunotherapy Shift Immune Microenvironment of Melanoma. International Journal of Nanomedicine, 17, 855-868.

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Comprehensive Western Blotting Protocol

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Western blotting was performed as described previously [13 (link)]. The following antibodies were used: CCN1 antibody (Abcam), β-actin antibody (Santa Cruz Biotechnology), Bcl-xL antibody (Cell Signaling Technology), c-Myc antibody (Cell Signaling Technology), Bax antibody (Santa Cruz Biotechnology), MEK antibody (Cell Signaling Technology), phospho-MEK antibody (Ser217/221, Cell Signaling Technology), ERK antibody (Cell Signaling Technology), phospho-ERK antibody (Thr202/Tyr204, Cell Signaling Technology), β-catenin antibody (Cell Signaling Technology), phospho-β-catenin antibody (Ser33/Ser37/Thr41, Cell Signaling Technology), and Survivin antibody (Cell Signaling Technology).
Gel electrophoresis and transfer as well as the chemiluminescence detection were conducted using the Bio-Rad Laboratories system.
Nuclear protein was extracted using a nuclear protein extraction kit (Beyotime, China), and an anti-Histone H1 antibody (Santa Cruz Biotechnology) was used as the loading control.

Niu C.C., Zhao C., Yang Z., Zhang X.L., Pan J., Zhao C, & Si W.K. (2014). Inhibiting CCN1 blocks AML cell growth by disrupting the MEK/ERK pathway. Cancer Cell International, 14, 74.

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Hippocampus Protein Extraction and Western Blot

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Frozen hippocampus tissue was homogenized in lysis buffer containing protease inhibitor cocktail purchased from Sigma (50 mM Tris-HCl, PH=7.4, 150 mM NaCl, 1 mM EDTA, 10 mM DTT, 0.1% SDS, 1% NP-40). The lysates were incubated on ice for 30 min and then followed by a centrifugation step at 17,000 g for 15 min at 4°C. The supernatants were collected and subjected to the Bradford Assay for protein concentration quantification (Thermo Scientific). Proteins were then added to modified Laemmli sample buffer contained DTT (150 mM final concentration) instead of 2-mercaptoethanol and denaturated at 95°C for 5 min. We loaded 20 μg protein into each lane on 10% precast Mini-Protean® TGXTM gradient gels (Bio-Rad). Proteins were transferred on to nitrocellulose membrane in 20% methanol transfer buffer with Criterion Blotter for 1 h at 100 volts. Membranes were blocked in 5% non-fat milk in PBS-T for 30 min. Bax antibody (1:200, Santa Cruz), Bcl-2 antibody (1:200, Santa Cruz), β-Actin antibody (1:500, Santa Cruz) were used for immunoblots. Secondary IgG horseradish peroxidase-linked whole antibodies from Donkey (1:5000, GE Healthcare) were used. Detection of protein was performed using AmershamTM ECLTM Prime Western Blotting Detection Reagent (GE Healthcare). Bands were visualized with ChemiDocTM MP Imaging System and quantified with quantity One software (Bio-Rad).

Zhang Y.P., Zhu Y.B., Duan D.D., Fan X.M., He Y., Su J.W, & Liu Y.L. (2015). Serum UCH-L1 as a Novel Biomarker to Predict Neuronal Apoptosis Following Deep Hypothermic Circulatory Arrest. International Journal of Medical Sciences, 12(7), 576-582.

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Western Blot Analysis of Apoptosis Markers

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For Western blot analysis, cytosolic and mitochondrial fractions were prepared as reported previously. Samples containing an equal amount of concentrated proteins were separated on 10% gradient SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride membrane by electroblotting for 90 min at 100 V and 4 °C. Non-specific membrane binding sites were blocked with blocking solution (PBS, 0.5% Tween-20, pH 7.4), containing 5% non-fat dry milk for 1 h at 4 °C. The membrane was incubated with primary mouse anti-Cyt c monoclonal antibody (Abcam, Cambridge, UK) diluted 1:1500 or goat anti-rabbit Bcl-2 (1:500, Santa Cruz, Santa Cruz, CA, USA) and Bax antibody (1:500, Santa Cruz) in blocking solution overnight at 4 °C. The membrane was washed thoroughly with PBS-T and then incubated for 1 h with horseradish peroxidase-conjugated anti-mouse (1:4000; Santa Cruz) or anti-rabbit IgG antibody (1:6000; Santa Cruz) in blocking solution, detected by chemiluminescence reagent plus, and exposed to film.

Zhong X., Yi X., da Silveira e Sá R.D., Zhang Y., Liu K., Xiao F, & Zhong C. (2017). CoQ10 Deficiency May Indicate Mitochondrial Dysfunction in Cr(VI) Toxicity. International Journal of Molecular Sciences, 18(4), 816.

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