Magnetic bead conjugated anti mouse cd8 53 6.7 monoclonal antibody

Spleen cells were obtained from 7 to 12 BALB/c mice that had been infected with the ME49 or CEP strain for 7 or 12 weeks in each experiment. Within a single experiment, all mice were infected for an identical period of time before harvesting their spleen cells. The spleen cells were suspended in Hanks’ balanced salt solution (Hyclone, Logan, UT) with 2% fetal bovine serum (Sigma, St. Louis, MO), and CD8⁺ immune T cells were purified from the spleen cells using magnetic bead-conjugated anti-mouse CD8 (53–6.7) monoclonal antibody (Miltenyi Biotech, Auburn, CA) [7 (link),16 (link)]. As a control, CD8⁺ normal T cells were also purified from the spleens of 8–12 uninfected BALB/c mice in the same manner. Infected SCID mice received the purified CD8⁺ T cells (3.5 or 7.5 × 10⁶ cells) intravenously at 3 weeks after infection [7 (link)].

Spleen cells obtained from 8–11 BALB/c mice that had been
infected for at least 2 months were pooled in each experiment and suspended in
Hanks’ balanced salt solution (Hyclone, Logan, UT) with 2% fetal
bovine serum (Sigma, St. Louis, MO). CD8⁺ immune T cells were
purified from the spleen cells using magnetic bead-conjugated anti-mouse CD8
(53-6.7) monoclonal antibody (Miltenyi Biotech, Auburn, CA) [20 (link), 5 (link)]. As a control, CD8⁺ normal T cells were
purified from the spleens of uninfected BALB/c mice in the same manner.
RAG1^−/−,
RAG1^−/−NOS2^−/−, and
SCID mice received the purified CD8⁺ T cells (2.4–3.2
× 10⁶ cells) intravenously at 3 weeks after infection
[20 (link)].