Kaempferide

Manufactured by Yuanye Bio-Technology

Sourced in China

Kaempferide is a pure chemical compound extracted from various plant sources. It functions as a core bioactive ingredient in biochemical analysis and research applications.

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3 protocols using kaempferide

Quantification of Flavonoids and Catechins

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Xanthine oxidase powder was purchased from Yuanye Biotechnology Co. (Shanghai, China). Flavone (99.0%), 7-OH flavone (98.0%), chrysin (98.0%), biochanin A (98.0%), naringin (98.0%), and myricetin (98.0%) were bought from Alfa Aesar (Thermo Fisher Scientific, MA, USA). Formononetin (99.0%) and genistein (99.0%) were acquired from Acros Organics (Fisher Scientific, PA, USA). Baicalin (98.0%), baicalein (98.0%), wogonin (98.0%), apigenin (98.0%), luteolin (98.0%), hispidulin (98.0%), tangeretin (98.0%), nobiletin (98.0%), galangin (98.0%), kaempferide (98.0%), kaempferol (98.0%), kaempferitrin (98.0%), quercetin (98.0%), fisetin (98.0%), rutin (98.0%), daidzein (98.0%), tectorigenin (98.0%), puerarin (98.0%), dihydromyricetin (98.0%), naringenin (98.0%), catechin (C; 98.0%), (−)-gallocatechin (GC; 98.0%), epicatechin, (EC; 98.0%), (−)-epicatechin gallate (ECG; 98.0%), (−)-epigallocatechin (EGC; 98.0%), (−)-epigallocatechin gallate (EGCG; 98.0%), and (−)-gallocatechin gallate (GCG; 98.0%) were commercially purchased from Yuanye Biotechnology Co. (Shanghai, China). High-performance liquid chromatography (HPLC) grade acetonitrile was purchased from Merck KGaA (Darmstadt, Germany). Ultrapure water (18.2 MΩ cm resistivity) was obtained from an ELGA water purification system (ELGA Berkefeld, Veolia, Germany). All other reagents and solvents were of analytical grade.

Yuan M., Liu Y., Xiao A., Leng J., Liao L., Ma L, & Liu L. (2019). The interaction of dietary flavonoids with xanthine oxidase in vitro: molecular property-binding affinity relationship aspects. RSC Advances, 9(19), 10781-10788.

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Quantifying Flavonoids in Plant Extracts

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Total flavonoid content was determined according to the method of Jin et al. [6 (link)]. In brief, the dry AR sample (0.1 g) was soaked in 10 mL of 70% ethanol, heated at 60 °C for 3 h, and filtered with a filter paper. The filtrate was used to determine the total flavonoid content via the aluminum nitrate colorimetric method at 510 nm using a spectrophotometer; rutin was used as the standard. The result was expressed as the equivalent of rutin per gram of the DW sample.
The method of Zhang et al. [42 (link)] was used to determine the contents of rutin, quercetin, and kaempferide using high-performance liquid chromatography with a C₁₅ column (4.6 × 250 mm, 5 μm, Thermo Scientific, Waltham, MA, USA) and ultraviolet–visible detector (SPD-15C, Shimadzu Co., Kyoto, Japan). The mobile phases were methanol (A) and 0.1% phosphorus solution (B). Gradient elution was performed as follows: 55% B for 0−15 min and 20% B for 15−30 min. The flow rate was 0.8 mL/min. rutin, quercetin, and kaempferide were detected at 366 nm (Figure 8). Standards of rutin (purity > 98%), quercetin (purity > 98%), and kaempferide (purity > 98%) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Jin M.Y., Wang M., Wu X.H., Fan M.Z., Li H.X., Guo Y.Q., Jiang J., Yin C.R, & Lian M.L. (2023). Improving Flavonoid Accumulation of Bioreactor-Cultured Adventitious Roots in Oplopanax elatus Using Yeast Extract. Plants, 12(11), 2174.

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MTT Assay for Cell Viability

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The general viability of the cultured cells was determined through the reduction of 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to formazan [20 (link)]. Exponentially growing B16F10 cells were trypsinized, harvested and equal numbers of cells (2 × 10⁵ cells/ml) in 100 μl medium were plated in 96-well microplates. After overnight incubation, 100 μl of different concentrations (8, 16, 32 μM) of Isorhamnetin and Kaempferide (purity >98%; Shanghai yuanye Bio-Technology Co., Ltd., China) was added to the well for 24 h. The untreated controls were exposed to fresh medium. Following this, 50 μl 5 mg/ml MTT solution was added to each well and incubated for 4 h. After aspirating the culture medium, the resulting formazan was dissolved with 150 μl dimethylsulfoxide (Sigma, USA). The plates were then placed on a shaker for 5 min and measured at 570 nm by using a microplate reader (Thermo Varioskan Flash 3001, USA).

Wang J.Y., Chen H., Wang Y.Y., Wang X.Q., Chen H.Y., Zhang M., Tang Y, & Zhang B. (2017). Network pharmacological mechanisms of Vernonia anthelmintica (L.) in the treatment of vitiligo: Isorhamnetin induction of melanogenesis via up-regulation of melanin-biosynthetic genes. BMC Systems Biology, 11, 103.

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