Human il 6 elisa kit

Manufactured by Diaclone

Sourced in France

The Human IL-6 ELISA Kit is a laboratory assay designed to quantitatively measure the levels of Interleukin-6 (IL-6) in human samples. It is an enzyme-linked immunosorbent assay (ELISA) that uses antibodies specific to IL-6 for detection and quantification.

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Human IL-6 High Sensitivity ELISA Kit (7 citations) ELISAs) (2 citations)

7 protocols using human il 6 elisa kit

Cytokine Quantification by ELISA

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The concentration of cytokines was estimated using ready-to-use immunoassays: IL-6 (Human IL-6 ELISA KIT, Diaclone SAS, Besancon CEDEX, France), IL-10 (Human IL-10 ELISA KIT, Diaclone SAS, Besancon CEDEX, France), TNF-α (Human TNF-α ELISA KIT, Diaclone SAS, Besancon CEDEX, France), and TGF-β1 (Human TGF-β1 ELISA KIT, Diaclone SAS, Besancon CEDEX, France). The measurements were made according to the manufacturer’s instructions. The cytokine concentrations were expressed in pg/mL or in ng/mL. The sensitivity of the methods, depending on the used calibrates, was 2 pg/mL for IL-6, 4.9 pg/mL for IL-10, 8.6 pg/mL for TGF-β1, and 8 pg/mL for TNF-α.
All the studied parameters were analyzed in duplicate, with sample means taken as the result.

Pawłowska M., Mila-Kierzenkowska C., Boraczyński T., Boraczyński M., Szewczyk-Golec K., Sutkowy P., Wesołowski R., Smoguła M, & Woźniak A. (2021). The Effect of Submaximal Exercise Followed by Short-Term Cold-Water Immersion on the Inflammatory State in Healthy Recreational Athletes: A Cross-Over Study. Journal of Clinical Medicine, 10(18), 4239.

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Cytokine and Oxidative Stress Biomarker Analysis

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Elisa kits used for measuring cytokines included Human IL-6 Elisa Kit (Diaclone, France) and Human IL-1β Elisa Kit (Zellbio, Germany). Laboratory kits used for measuring the oxidative stress biomarkers (except ROS) included SOD Activity Kit (KSOD96), CAT Activity Kit (KCAT96), Thiol Content Assay Kit (KTHI96), Total Antioxidant Assay Kit (KTAC96), Lipid Peroxidation Kit (KMDA96), and Protein Carbonyl Assay Kit (KPCA96) were purchased from KIAZIST Company, Iran. 2'-7'dichlorofluorescin diacetate (H2DCFDA) (D399, Invitrogen, USA) was used to assess Cellular ROS. Platelet agonist used to activate platelet was thrombin receptor activator peptide (TRAP-6 (SFLLRN), 4,031,274.0005; Bachem, Germany) and antibodies used for flow cytometric measurement of platelet function were FITC Mouse Anti-Human CD62P (Clone AK-4) (555,523, BD Pharmingen, USA) and FITC Mouse IgG1, κ Isotype Control (Clone MOPC-21) (555,748, BD Pharmingen, USA). The instruments used for the laboratory measurements were as follow: 3-30 K Centrifuge (Sigma, Germany), FACSCalibure Flow Cytometer (BD Pharmingen, USA), UV2100 spectrophotometer (UNICO, China), Infinite M200 plate reader (Tecan, Austria), and ELISA reader apparatus (BD Biosciences, USA).

Gohari S., Reshadmanesh T., Khodabandehloo H., Karbalaee-Hasani A., Ahangar H., Arsang-Jang S., Ismail-Beigi F., Dadashi M., Ghanbari S., Taheri H., Fathi M., Muhammadi M.J., Mahmoodian R., Asgari A., Tayaranian M., Moharrami M., Mahjani M., Ghobadian B., Chiti H, & Gohari S. (2022). The effect of EMPAgliflozin on markers of inflammation in patients with concomitant type 2 diabetes mellitus and Coronary ARtery Disease: the EMPA-CARD randomized controlled trial. Diabetology & Metabolic Syndrome, 14, 170.

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Measurement of IL-6 and Anti-dsDNA

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The IL-6 was measured using Human IL-6 ELISA Kit (Diaclone, France) as per manufacturer instructions. IMTEC-dsDNA-Antibodies ELISA kit (Germany) was used for measuring anti-dsDNA titre, according to the manufacturer instructions.

Ramesh R., Sundaresh A., Rajkumar R.P., Negi V.S., Vijayalakshmi M.A., Krishnamoorthy R., Tamouza R., Leboyer M, & Kamalanathan A.S. (2021). DNA hydrolysing IgG catalytic antibodies: an emerging link between psychoses and autoimmunity. NPJ Schizophrenia, 7, 13.

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Plasma Biomarker Quantification

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The whole blood was centrifugated (1,000 g at 4°C for 10 min) to obtain plasma. Adiponectin, IL-6, and TNF-α assays were performed using the HUMAN ADP/Acpr30 ELISA Kit Elabscience (Cat #E-EL-H6122), Human IL-6 ELISA KIT Diaclone (Cat #950.030.192), and TNF-α ELISA KIT DIACLONE (Cat #950.090.192) according to manufacturer's protocols respectively.

Fontanella R.A., Scisciola L., Rizzo M.R., Surina S., Sardu C., Marfella R., Paolisso G, & Barbieri M. (2021). Adiponectin Related Vascular and Cardiac Benefits in Obesity: Is There a Role for an Epigenetically Regulated Mechanism?. Frontiers in Cardiovascular Medicine, 8, 768026.

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Evaluating THGP's Effect on IL6 Production Under Oxidative Stress

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The effect of THGP on the production of IL6 was evaluated under oxidative stress conditions, which were induced by the direct addition of hydrogen peroxide to the cells. The procedure used for the cell seeding and oxidative stress treatment was the same as that described in “Measurement of cell proliferation by MTS and ATP production under oxidative stress induced by direct hydrogen peroxide addition”. The experimental group consisted of 3 groups: untreated group, oxidative stress treated group and oxidative stress-THGP treated group. The THGP treatment was only performed at a final concentration of 5.9 mM. After the oxidative stress treatment, the culture supernatants were collected and used as a sample for measuring the production of IL6. IL6 was measured using a Human IL-6 ELISA Kit (Diaclone, Besançon, France) according to the manufacturer’s instructions. Viable cells were evaluated via a MTS assay, and the amount of IL6 culture supernatant was corrected by the viable cell rate.

Takeda T., Doiyama S., Azumi J., Shimada Y., Tokuji Y., Yamaguchi H., Nagata K., Sakamoto N., Aso H, & Nakamura T. (2019). Organogermanium suppresses cell death due to oxidative stress in normal human dermal fibroblasts. Scientific Reports, 9, 13637.

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Plasma IL-6 Quantification Using ELISA

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The plasma levels of IL6 were assessed using the “Human IL-6 ELISA Kit” (Diaclone, Besançon, France) following manufacturer instructions.

Sundaresh A., Oliveira J., Chinnadurai R.K., Rajkumar R.P., Hani L., Krishnamoorthy R., Leboyer M., Negi V.S, & Tamouza R. (2019). IL6/IL6R genetic diversity and plasma IL6 levels in bipolar disorder: An Indo-French study. Heliyon, 5(1), e01124.

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Measuring Serum IL-6 in Surgical Patients

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Blood samples were assessed for IL-6 at three time intervals: following induction of anesthesia (baseline), at the end of surgery following desufflation of the abdomen, and at 24 h postoperatively. The sample of 5–10 ml of blood was collected aseptically and allowed to stand at room temperature for 1 h to clot. Serum was removed and stored at −80°C till assayed on a 96-well plate using commercially available enzyme-linked immunosorbent assay (Human IL-6 ELISA Kit, Diaclone^®).

Arora V., Tyagi A., Kumar S., Kakkar A, & Das S. (2017). Intraoperative low tidal volume ventilation strategy has no benefits during laparoscopic cholecystectomy. Journal of Anaesthesiology, Clinical Pharmacology, 33(1), 57-63.

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