Electrochemical measurements were performed using a µStat 200 bipotentiostat controlled by DropView software (DropSens, Oviedo, Spain). A DRP-BICAST connector was used to interface the bipotentiostat and the customized dual screen-printed carbon electrodes (dual-SPCE, DRP-X1110, DropSens, incorporating two carbon elliptic-shaped working electrodes (WE, A = 0.063 cm2), a carbon auxiliary electrode and a silver pseudo-reference electrode).
For sample preparation, a block thermostat (Tembloc, Selecta, Barcelona, Spain) and two centrifuges (Heraues Megafuge 16R and Heraeus-Fresco 21, Thermo Fisher Scientific, Osterode am Harz, Germany) were used.
The evaluation of the accuracy of the assay’s results was performed using ELISA kits. For these analyses, a multi-mode microplate reader (Synergy HT W/TRF, BioTek Instruments, Winooski, VT, USA) was used, and the data were treated with Gen5 Version 2.0 data analysis software (BioTek Instruments, Winooski, VT, USA).
For sample preparation, a block thermostat (Tembloc, Selecta, Barcelona, Spain) and two centrifuges (Heraues Megafuge 16R and Heraeus-Fresco 21, Thermo Fisher Scientific, Osterode am Harz, Germany) were used.
The evaluation of the accuracy of the assay’s results was performed using ELISA kits. For these analyses, a multi-mode microplate reader (Synergy HT W/TRF, BioTek Instruments, Winooski, VT, USA) was used, and the data were treated with Gen5 Version 2.0 data analysis software (BioTek Instruments, Winooski, VT, USA).