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Diet 5013

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Diet 5013 is a laboratory animal diet product. It is designed to provide a complete and balanced nutrition for laboratory animals.

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Lab products found in correlation

Isocage p, by Tecniplast (2 mentions)

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Rodent breeder diet 5013 (2 citations) Autoclavable Rodent Breeder Diet #5013 (2 citations)

2 protocols using diet 5013

1

Establishing C. difficile Infection in HMA Mice

Cited 1 time
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The HMA mice were housed in positive-pressure individually ventilated cages (IVCs) (ISOcage P; Techniplast, West Chester, PA) in order to prevent cross-contamination and maintain their gnotobiotic status 49 , 50 . All mice were fed a sterilized rodent breeder diet 5013 (LabDiet, St. Louis, MO). 8- to 16-week-old female and male mice were used in all experiments. All animals were handled in accordance with the protocols approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Michigan. To attain robust C. difficile infection in HC-HMA mice, the animals were treated with a cocktail of antibiotics ofr 5 days. The cocktail contains kanamycin (0.4 mg/ml), gentamicin (0.035 mg/ml), colistin (850 U/ml), metronidazole, (0.215 mg/ml), and vancomycin (0.045 mg/ml). Clindamycin (10 mg/kg) was then injected intra-peritoneally at day 5. One day after clindamycin injection, mice were challenged by C. difficile 24 h post-IP injection 17 (link).
Nagao-Kitamoto H., Leslie J.L., Kitamoto S., Jin C., Thomsson K.A., Gillilland MG I.I.I., Kuffa P., Goto Y., Jenq R.R., Ishii C., Hirayama A., Seekatz A.M., Martens E.C., Eaton K.A., Kao J.Y., Fukuda S., Higgins P.D., Karlsson N.G., Young V.B, & Kamada N. (2020). Interleukin-22-mediated host glycosylation prevents Clostridioides difficile infection by modulating the metabolic activity of the gut microbiota. Nature medicine, 26(4), 608-617.
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2

Establishing C. difficile Infection in HMA Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The HMA mice were housed in positive-pressure individually ventilated cages (IVCs) (ISOcage P; Techniplast, West Chester, PA) in order to prevent cross-contamination and maintain their gnotobiotic status 49 , 50 . All mice were fed a sterilized rodent breeder diet 5013 (LabDiet, St. Louis, MO). 8- to 16-week-old female and male mice were used in all experiments. All animals were handled in accordance with the protocols approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Michigan. To attain robust C. difficile infection in HC-HMA mice, the animals were treated with a cocktail of antibiotics ofr 5 days. The cocktail contains kanamycin (0.4 mg/ml), gentamicin (0.035 mg/ml), colistin (850 U/ml), metronidazole, (0.215 mg/ml), and vancomycin (0.045 mg/ml). Clindamycin (10 mg/kg) was then injected intra-peritoneally at day 5. One day after clindamycin injection, mice were challenged by C. difficile 24 h post-IP injection 17 (link).
Nagao-Kitamoto H., Leslie J.L., Kitamoto S., Jin C., Thomsson K.A., Gillilland MG I.I.I., Kuffa P., Goto Y., Jenq R.R., Ishii C., Hirayama A., Seekatz A.M., Martens E.C., Eaton K.A., Kao J.Y., Fukuda S., Higgins P.D., Karlsson N.G., Young V.B, & Kamada N. (2020). Interleukin-22-mediated host glycosylation prevents Clostridioides difficile infection by modulating the metabolic activity of the gut microbiota. Nature medicine, 26(4), 608-617.
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