The effect of EFL3 on phospholipid content was measured following the methods reported before [22 (link)]. Mycelia were cultured as described above in Section 2.6 . After being collected and washed with sterilized distilled water, mycelia samples were ground with liquid nitrogen. Subsequently, 10 mg mycelia per sample were collected and resuspended in the assay buffer. The phospholipid content of P. capsici mycelia were measured using a phospholipid assay kit (Sigma-Aldrich, St. Louis, MO, USA). The effect of EFL3 on cellulose content was also determined. Mycelia were cultured and collected as described above. After being ground with liquid nitrogen, 0.3 g of mycelia per sample were collected and resuspended in the extraction buffer. The cellulose content of P. capsici mycelia were measured by a cellulose assay kit (Solarbio, Beijing, China). The experiment was repeated three times.
Cellulose assay kit
Manufactured by Solarbio
Sourced in China
The Cellulose Assay Kit is a laboratory equipment product designed to quantify the amount of cellulose present in a sample. It provides a standardized method for the colorimetric determination of cellulose content.
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Lab products found in correlation
3 protocols using cellulose assay kit
1
Phospholipid and Cellulose Content Analysis in P. capsici Mycelia
2
Soil Enzymatic Activity Profiling
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To measure the enzymatic activities, fresh air-dried samples were prepared. Cellulose, urease, dehydrogenase and alkaline phosphatase were determined by Cellulose Assay Kit (BC0125, Solarbio, Beijing, China), Urease (UE) Assay Kit (BC0155, Solarbio, Beijing, China), Dehydrogenase (UE) Assay Kit (BC0395, Solarbio, Beijing, China) and alkaline phosphatase Assay Kit (BC0280, Solarbio, Beijing, China), respectively. All measurements were performed according to the manufacturer’s instructions.
3
Cellulose Extraction and Quantification
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Cellulose extraction and measurement were performed using a cellulose assay kit
(Solarbio, China). The fiber samples (300 mg) were frozen in liquid nitrogen and
lyophilized, then washed with 0.5-M ice-cold potassium phosphate buffer (0.5 mL, pH 7.0) 3
times. The pellets were washed with deionized water, dispersed in 80% (v/v) ethanol, and
incubated at 90°C for 20 min. After cooling to room temperature, the samples were
centrifuged at 6,000 g for 10 min at room temperature. The pellets were
then washed twice, first with 80% (v/v) ethanol and then with acetone. An appropriate
amount of deamylase was then added and incubated for 30 min at 37°C to remove starch, the
samples were centrifuged at 6,000 g for 20 min at room temperature, and
the pellets were dried at 50°C. The pellets were dissolved with 72% (v/v)
H2SO4, and centrifuged for 10 min. The supernatants were then
diluted with H2O and the working solution was added to calculate the cellulose
contents by measuring the absorbance at 620 nm.
(Solarbio, China). The fiber samples (300 mg) were frozen in liquid nitrogen and
lyophilized, then washed with 0.5-M ice-cold potassium phosphate buffer (0.5 mL, pH 7.0) 3
times. The pellets were washed with deionized water, dispersed in 80% (v/v) ethanol, and
incubated at 90°C for 20 min. After cooling to room temperature, the samples were
centrifuged at 6,000 g for 10 min at room temperature. The pellets were
then washed twice, first with 80% (v/v) ethanol and then with acetone. An appropriate
amount of deamylase was then added and incubated for 30 min at 37°C to remove starch, the
samples were centrifuged at 6,000 g for 20 min at room temperature, and
the pellets were dried at 50°C. The pellets were dissolved with 72% (v/v)
H2SO4, and centrifuged for 10 min. The supernatants were then
diluted with H2O and the working solution was added to calculate the cellulose
contents by measuring the absorbance at 620 nm.
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