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Ezrin

Manufactured by BD
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Sourced in Canada, Denmark

Ezrin is a versatile laboratory equipment used for various applications in scientific research and analysis. It serves as a tool for the isolation, purification, and detection of specific biomolecules, such as proteins, from complex biological samples. The core function of Ezrin is to facilitate the separation and identification of target analytes, enabling researchers to obtain valuable insights and data for their investigations.

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Lab products found in correlation

Moesin, by Cell Signaling Technology (1 mentions) Nherf1, by Thermo Fisher Scientific (1 mentions) Nf2 c terminal 1 800, by Santa Cruz Biotechnology (1 mentions) Aperio scanscope cs2 whole slide image system, by Leica (1 mentions) Ventana benchmark xt, by Roche (1 mentions) Ultraview dabv3 kit, by Roche (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) β1 integrin, by BD (1 mentions) Py416 c src, by Cell Signaling Technology (1 mentions) Gm130, by BD (1 mentions) 510 confocal microscope, by Zeiss (1 mentions) β catenin, by Santa Cruz Biotechnology (1 mentions) Nherf1, by Abcam (1 mentions) Ki 67, by Abcam (1 mentions) Jam a, by BD (1 mentions) Cleaved caspase 8, by Cell Signaling Technology (1 mentions) Becn1, by Abcam (1 mentions) Cftr clone m3a7, by Abcam (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Pparγ, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Mouse anti-ezrin (3 citations) Anti-ezrin (2 citations) Monoclonal anti-Ezrin (2 citations)

4 protocols using ezrin

1

Immunohistochemical Staining of Neural Markers

Cited 1 time
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The specimens were processed for H& E staining and IHC as described [8 (link)], with antibodies for NHERF1 1:3200 (Thermo/Fisher, Waltham, MA), moesin 1:100 (3150, Cell Signaling Technology, Danvers, MA), Kir7.1 1:200, NF2 C-terminal 1:800 (C18) (see also [19 (link)]) (Santa Cruz Biotechnology, Santa Cruz, CA) and ezrin 1:400 (30252, BD Biosciences, San Jose, CA). Two certified neuropathologists reviewed independently the IHC results. Images were acquired and analyzed at various magnifications with an Aperio Scanscope CS2 whole slide image system (Leica Biosystems, San Diego, CA).
Georgescu M.M., Mobley B.C., Orr B.A., Shang P., Lehman N.L., Zhu X., O’Neill T.J., Rajaram V., Hatanpaa K.J., Timmons C.F, & Raisanen J.M. (2016). NHERF1/EBP50 and NF2 as diagnostic markers for choroid plexus tumors. Acta Neuropathologica Communications, 4, 55.
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2

Ezrin and CK20 Immunostaining Protocol

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Sections are immunostained with ezrin (BD Biosciences, 610603; 1:100) or CK20-specific mouse monoclonal antibody (clone Ks20.8, Dako, Glostrup, Denmark). Stainings are performed with Ventana BenchMark XT immunostainer (ventana Medical Systems, Tucson, AZ) using an UltraView DABv3 kit (Ventana). The chromogene is 3,3′-diaminobenzidine in all the stainings.
Pagès D.L., Dornier E., de Seze J., Gontran E., Maitra A., Maciejewski A., Wang L., Luan R., Cartry J., Canet-Jourdan C., Raingeaud J., Lemahieu G., Lebel M., Ducreux M., Gelli M., Scoazec J.Y., Coppey M., Voituriez R., Piel M, & Jaulin F. (2022). Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments. Science Advances, 8(39), eabp8416.
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3

Immunofluorescence Analysis of 3D Calu-3 Cultures

Cited 1 time
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Calu-3 in 3D culture were stained essentially as described for MDCK cysts [9 (link)]. Primary antibodies utilized were: Ki-67 (Epitomics, 2642-1), Cleaved Caspase 3 (Cell Signaling Technology, 9661L), JAM-A (BD Biosciences, 612120), Muc1 (Epitomics, 2900-1), GM130 (BD Biosciences, C65120), β-catenin (Santa Cruz, sc-7199), NHERF1 (Abcam, ab3452), Ezrin (BD Biosciences, 610603), Cleaved Caspase 8 (Cell Signaling Technology, 9496), β1-integrin (BD Biosciences, 610467), pY416-c-Src (Cell Signaling Technology, 6943P), pY1105-p190 (Abcam, ab55339). Alexa fluorophore-conjugated secondary antibodies (1:250) or Phalloidin (1:200) (both Invitrogen) and Hoechst to label nuclei (10 μg/ml), were utilized. Imaging was performed on a Zeiss 510 Confocal Microscope, using a 63x oil immersion lens. Image processing was performed using ImageJ. Images shown are representative of 3 separate experiments.
Datta A., Sandilands E., Mostov K.E, & Bryant D.M. (2017). Fibroblast-derived HGF drives acinar lung cancer cell polarization through integrin-dependent RhoA-ROCK1 inhibition. Cellular signalling, 40, 91-98.
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4

Western Blot Analysis of Cellular Proteins

Cited 3 times
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The whole lysate or membrane fraction proteins of cell lines and mice intestine homogenates were obtained from treated and untreated cells or mice as described [12 (link),13 (link),15 (link),18 (link),19 (link),58 (link),75 (link)–79 (link)]. The equal amount of protein were resolved by SDS-PAGE gel and blotted with antibodies against: SQSTM1, (Sigma Aldrich, 108k4767)1:1000, PPARγ (Santa Cruz Biotechnology, sc7273) 1:500, BECN1 (Abcam, ab58878) 1:1000, CFTR clone M3A7 (Abcam, ab4067) 1:500, phospho- ERK1/2 (php42/44, Cell Signaling Technology, #91101) 1:1000, Ezrin (BD, 610603) 1:1000, biotin (Abcam, ab1227 )1:2000, TG2( CUB Novus Bio) 1:1000 used as primary antibodies. Normalization was performed by probing the membrane with anti-β-actin (Cell Signaling, #4970) 1:1000, and anti-flotillin (Abcam ab15148) 1:1000 antibodies.
Esposito S., Villella V.R., Ferrari E., Monzani R., Tosco A., Rossin F., D’Eletto M., Castaldo A., Luciani A., Silano M., Bona G., Marseglia G.L., Romani L., Piacentini M., Raia V., Kroemer G, & Maiuri L. (2019). Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease. Aging (Albany NY), 11(7), 2003-2019.
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