Rabbit anti caveolin 1 antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Rabbit anti-caveolin-1 antibody is a primary antibody used for the detection and analysis of caveolin-1 in various biological samples. Caveolin-1 is a structural component of caveolae, which are invaginations of the plasma membrane involved in cellular processes such as signal transduction and endocytosis. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of caveolin-1 in cells and tissues.

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Lab products found in correlation

Rabbit anti sirt1 antibody, by Santa Cruz Biotechnology (1 mentions) Rabbit anti vegf antibody, by Santa Cruz Biotechnology (1 mentions) Mouse anti c myc antibody, by Santa Cruz Biotechnology (1 mentions) Rabbit anti c myc antibody, by Merck Group (1 mentions) Rabbit anti pdgfr α antibody, by Santa Cruz Biotechnology (1 mentions) Alexa 488 goat anti rabbit alexa 568 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Vectashield mounting medium for fluorescence h 1000, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Polyclonal rabbit anti-caveolin-1 antibody Rabbit anti-caveolin-1 Anti-caveolin-1 antibody Caveolin-1 antibody Anti-caveolin-1 Caveolin-1 Rabbit anti-TM

3 protocols using rabbit anti caveolin 1 antibody

Antibody Validation for Cellular Targets

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The antibodies used in this study were primary polyclonal Rabbit anti-caveolin-1 antibody (Santa Cruz biotechnology), primary polyclonal Rabbit anti-VEGF antibody (Santa Cruz Biotechnology), primary polyclonal Rabbit anti-SIRT1 antibody (Santa Cruz Biotechnology), primary polyclonal Goat anti-Ubc9 N-15 antibody (Santa Cruz Biotechnology), primary monoclonal mouse anti-β-Actin antibody (Santa Cruz Biotechnology), primary monoclonal mouse anti-ER-α antibody (Santa Cruz Biotechnology).

Xu J., Shumate C., Qin Y., Reddy V., Burnam Y., Lopez V., Okoli J., P Reddy E.S, & Rao V.N. (2017). A novel Ubc9 -dependent pathway regulates SIRT1- ER-α Axis and BRCA1-associated TNBC lung metastasis. Integrative molecular medicine, 4(4), 10.15761/IMM.1000298.

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Characterization of GD3 and GD2 Antibodies

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Mouse anti‐GD3 mAb (R‐24) was obtained from Dr. L.J. Old (Memorial Sloan Kettering Cancer Center, New York, NY, USA). Mouse anti‐GD2 mAb (220‐51) was generated in our laboratory. Rabbit anti‐flotillin‐1 antibody, rabbit anti‐caveolin‐1 antibody, mouse anti‐CD109 mAb, rabbit anti‐PDGFRα antibody, goat anti‐ASCT2 antibody, and mouse anti‐c‐myc antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti‐ASCT2 antibody, rabbit anti‐phospho‐mTOR antibody (Ser2448), rabbit anti‐mTOR mAb, mouse anti‐phospho‐p70 S6 kinase mAb (Thr389), rabbit anti‐p70 S6 kinase mAb, rabbit anti‐S6 ribosomal protein mAb, rabbit anti‐phospho‐S6 ribosomal protein mAb (Ser235/Ser236), rabbit anti‐EphA2 mAb, and rabbit anti‐CD44 antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Goat anti‐urokinase plasminogen activator surface receptor antibody was purchased from R&D Systems (Minneapolis, MN, USA). Rabbit anti‐c‐myc antibody was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Rabbit anti‐ASCT2 conjugated with Alexa 488 was purchased from Bioss (Woburn, MA, USA).

Esaki N., Ohkawa Y., Hashimoto N., Tsuda Y., Ohmi Y., Bhuiyan R.H., Kotani N., Honke K., Enomoto A., Takahashi M., Furukawa K, & Furukawa K. (2018). ASC amino acid transporter 2, defined by enzyme‐mediated activation of radical sources, enhances malignancy of GD2‐positive small‐cell lung cancer. Cancer Science, 109(1), 141-153.

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Immunofluorescence analysis of caveolin-1 and SIRT1

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MCF10A, HCC1937, HCC1937 BRCA1 and CAL51 cells were cultured alone or transfected with pCDNA3, BRCA1a, BRCA1a Mut#1, BRCA1a Mut#4, BRCA1a Mut#9 or EGFP-Antisense Ubc9 for 24 hours in six-well plates onto glass coverslips overnight. The cells were washed and fixed in icy methanol for 5 minute, and blocked using 10% BSA for 60 min, followed by primary polyclonal Rabbit anti-caveolin-1 antibody 1:150, primary polyclonal anti-SIRT1 antibody (Santa Cruz),l:150 diluted in 1.5% BSA made in PBS at 25°C for 1hr and Alexa 488 goat anti-Rabbit/Alexa 568 goat anti-mouse (Molecular Probes) diluted in 1.5% BSA/PBS for 50 min and stained (Hoechst 33258, Pentahydrate, Life technologies). The cover slips were mounted with Vectashield mounting medium for fluorescence (H-1000 from Vector). The stained cells were examined by LSM 700 Confocal Microscope, equipped with 63× oil Ph immersion objectives. Composite filter cubes were used for the 488–405 as described previously [27 (link)].

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