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Kta pure fplc unit

Manufactured by GE Healthcare
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The ÄKTA Pure FPLC unit is a versatile liquid chromatography system designed for protein purification and analysis. It is capable of performing Fast Protein Liquid Chromatography (FPLC) techniques, which are used to separate and purify various biomolecules, including proteins, peptides, and nucleic acids. The ÄKTA Pure FPLC unit provides precise control over flow rates, pressure, and other parameters, enabling efficient and reproducible purification processes.

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Lab products found in correlation

Superose 6 10 300, by GE Healthcare (2 mentions) Cholesterol, by Fujifilm (1 mentions) Mouse insulin elisa kit, by Thermo Fisher Scientific (1 mentions) Protease inhibitor, by Thermo Fisher Scientific (1 mentions) Hr series nefa hr, by Fujifilm (1 mentions) Nc9138103, by Fujifilm (1 mentions)

Spelling variants of this product

ÄKTA pure (104 citations) ÄKTA FPLC (62 citations) ÄKTA Pure FPLC (22 citations) Pure FPLC (2 citations)

2 protocols using kta pure fplc unit

1

Liver Lipid Extraction and Lipoprotein Analysis

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Livers were disrupted in homogenization buffer (10 mM Tris-HCl [Bioland], pH 7.4, 150 mM NaCl [Sigma-Aldrich], 1 mM EDTA [Bioland], and protease inhibitors [Thermo Fisher Scientific]) and lipids were extracted from 0.5–1 mg liver protein using the Folch method (26 (link)). Liver and plasma cholesterol and triglyceride were measured by colorimetric assay (cholesterol, WAKO; TG, Sekisui; glycerol, Cayman [biomol]; NEFA, Fujifilm, HR Series NEFA-HR). Plasma insulin was measured by Mouse Insulin ELISA Kit (Thermo Fisher Scientific). To resolve lipoprotein classes, plasma samples were injected into a Superose 6 10/300 (GE Healthcare Life Sciences) column attached to ÄKTA Pure FPLC unit (GE Healthcare) and fractions were collected for measurement of cholesterol by colorimetric assay (WAKO).
Xiao X., Kennelly J.P., Feng A.C., Cheng L., Romartinez-Alonso B., Bedard A., Gao Y., Cui L., Young S.G., Schwabe J.W, & Tontonoz P. (2024). Aster-B–dependent estradiol synthesis protects female mice from diet-induced obesity. The Journal of Clinical Investigation, 134(4), e173002.
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2

Lipid Extraction and Cholesterol Quantification

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Tissues were disrupted in homogenization buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA and protease inhibitors) and lipids were extracted from 0.5–1 mg liver protein using the Folch method44 (link). Fecal samples were pulverized using a mortar and pestle and lipids were extracted from 200 mg feces using the Folch method. Liver and fecal cholesterol were measured by colorimetric assay (WAKO, NC9138103). Plasma total cholesterol (WAKO, NC9138103), TG (Sekisui, 36-100-4169) and non-esterified fatty acids (WAKO, 991-34891) were measured using colorimetric assays. To resolve lipoprotein classes, plasma samples were injected into a Superose 6 10/300 (GE Healthcare Life Sciences) column attached to ÄKTA Pure FPLC unit (GE Healthcare) and fractions were collected for measurement of cholesterol by colorimetric assay (WAKO, NC9138103).
Xiao X., Kennelly J.P., Ferrari A., Clifford B.L., Whang E., Gao Y., Qian K., Sandhu J., Jarrett K.E., Brearley-Sholto M.C., Nguyen A., Nagari R.T., Sub Lee M., Zhang S., Weston T.A., Young S.G., Bensinger S.J., Villanueva C.J., de Aguiar Vallim T.Q, & Tontonoz P. (2023). Hepatic nonvesicular cholesterol transport is critical for systemic lipid homeostasis. Nature metabolism, 5(1), 165-181.
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