Ib meca

Manufactured by Bio-Techne

Sourced in United Kingdom

IB-MECA is a laboratory equipment product offered by Bio-Techne. It is a compact and automated device designed for immunoblotting and membrane-based protein detection applications in research settings. The core function of IB-MECA is to perform efficient and consistent western blot and membrane-based assays.

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2-Cl-IB-MECA (4 citations) Cl-IB-MECA (4 citations)

6 protocols using ib meca

Adenosine Receptor Ligand Preparation

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Cyclopentyladenosine (CPA), 5’-(N-ethylcarboxamido)adenosine (NECA), and 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX) were purchased from Sigma-Aldrich, Munich, Germany. 2-[4-(2-carboxyethyl)phenethylamino]-5′-N-ethylcarboxamidoadenosine (CGS-21680), 1-deoxy-1-(6-[([3-lodophenyl]methyl)-amino]-9H-purin-9-yl)-N-methyl-β-D-ribofuranuronamide (IB-MECA) and N-(4-Acetylphenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1, 3-dipropyl-1H-purin-8-yl)phenoxy]acetamide (MRS 1706) were obtained from Tocris (Distributed by BIOZOL Diagnostica, Eching, Germany). Stock solutions (5 mM up to 100mM) were prepared in Ethanol (CPA) or DMSO (all other) and stored at –20°C. Further dilutions were carried out in distilled water.

Eusemann T.N., Willmroth F., Fiebich B., Biber K, & van Calker D. (2015). Adenosine Receptors Differentially Regulate the Expression of Regulators of G-Protein Signalling (RGS) 2, 3 and 4 in Astrocyte-Like Cells. PLoS ONE, 10(8), e0134934.

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Characterization of A3 Adenosine Receptor

Cited 1 time

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HEK293T cells (passage 20) were kindly provided by Prof. O. De Wever
(Laboratory of Experimental Cancer Research, Department of Radiation Oncology
and Experimental Cancer Research, Ghent University Hospital, Belgium). The human
A₃AR construct (NM_000677.2,
transcript variant 2 of the ADORA3 gene) and human βarr2
construct (NM_004313) were purchased from
Origene Technologies (Rockville, MD, USA). DOTAP Liposomal Transfection Reagent
was purchased from Sigma Aldrich (Steinheim, Germany), and the anti-dNGFR
antibody was purchased from Chromaprobe (Maryland Heights, MO, USA). Reference
agonists 2-Cl-IB-MECA, IB-MECA, CGS21680 and NECA were purchased from Tocris
Bioscience (Bio-techne, Abingdon, UK). All other chemicals and reagents used
were purchased from the same suppliers as described previously [18 (link)] or synthesized at NIDDK, National Institutes of
Health as reported [19 (link)–33 (link)].

Storme J., Tosh D.K., Gao Z.G., Jacobson K.A, & Stove C.P. (2018). Probing Structure-Activity Relationship in β-Arrestin2 Recruitment of Diversely Substituted Adenosine Derivatives. Biochemical pharmacology, 158, 103-113.

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Evaluating Analgesic Effects of A3AR and H4R Agonists

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The selective A₃AR agonist, IB-MECA (0.5–1 mg/kg; Tocris Bioscience, Milan, Italy), and the selective H₄R agonist, VUF 8430 (10–30 mg/kg; Tocris Bioscience, Milan, Italy) were dissolved in sterile saline solution and intraperitoneally (i.p.) administered on day 8 after CCI surgery. Control animals received an equal volume of vehicle. Behavioral measurements were performed before and 30 min, 1 h, 2 h, 3 h and 5 h after compounds injection.

Micheli L., Durante M., Lucarini E., Sgambellone S., Lucarini L., Di Cesare Mannelli L., Ghelardini C, & Masini E. (2021). The Histamine H4 Receptor Participates in the Anti-Neuropathic Effect of the Adenosine A3 Receptor Agonist IB-MECA: Role of CD4+ T Cells. Biomolecules, 11(10), 1447.

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Fluorescent Binding Assay for A2A Receptor

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CGS 21680 hydrochloride, IB-MECA, SCH 442416 and PSB-10 hydrochloride were purchased from Tocris Bioscience (Bristol, UK). Forskolin was purchased from Sigma-Aldrich (St. Louis, MO, USA). For non-radioactive binding assays, tag-lite buffer (TLB) was obtained from Cisbio Bioassays (LABMED, Codolet, France). The Tb derivative of O6-benzylguanine is commercialized as HaloTag-Lumi4-Tb (SHALOTBC, Cisbio Bioassays, LABMED, Codolet, France). A_2AR antagonist (SCH 442416 derivate) conjugated to a red fluorescent probe was purchased from Cisbio Bioassays (L0058RED, LABMED, Codolet, France). The intellectual property of TLB components and of fluorophore structure is owned by Cysbio Bioassays.

Lillo A., Martínez-Pinilla E., Reyes-Resina I., Navarro G, & Franco R. (2020). Adenosine A2A and A3 Receptors Are Able to Interact with Each Other. A Further Piece in the Puzzle of Adenosine Receptor-Mediated Signaling. International Journal of Molecular Sciences, 21(14), 5070.

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Adenosine Receptor Signaling Assay

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Adenosine, AMP (Adenosine 5’-monophosphate disodium salt), DPCPX (8-cyclopentyl-1,3-dipropylxanthine), EHNA hydrochloride, pentostatin (dCF), and dipyridamole (DPM) were obtained from Sigma Aldrich (St Louis, MO). PD 184352 (2-[(2-chloro-4-iodophenyl)amino]-N-cyclopropylmethoxy)-3,4-difluorobenzamide), U 73122 (1-[6-[[(17β)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole -2,5-dione), ANR 94 (8-ethoxy-9-ethyl-9H-purin-6-amine), PSB 603 (8-[4-[4-(4-Chloro phenzyl)piperazide-1-sulfonyl)phenyl]]-1-propylxanthine), MRS 1220 (N-[9-chloro-2-(2-fura nyl)[1 (link),2 (link),4 ]-triazolo[1,5-c]quinazolin-5-yl]benzene acetamide), pertussis toxin (50 ng/ml), IB-MECA (1-deoxy-1-[6-[[(3-iodophenyl)methyl]amino]-9H-purin-9-yl]-N-methyl-β-D-ribofuranuronamide), and CPA (N-cyclopentylAdenosine) were purchased from Tocris (Ellisville, MO). SDF-1 was from PeproTech (London, UK).

Tarnowski M., Tkacz M., Piotrowska K., Zgutka K, & Pawlik A. (2018). Differential effect of adenosine on rhabdomyosarcoma migration and proliferation. Archives of Medical Science : AMS, 16(2), 414-427.

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Effects of Sunitinib and IB-MECA on HL60 Cells

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The HL60 cell line were obtained from European Collection of Cell Culture (England). RPMI 1640 medium and MTT was purchased from Sigma Aldrich (USA) and the medium supplements L-Glutamine, HEPES and antibiotics mix (100 U/ml penicillin and 100 µg/ml of streptomycin) were from Invitrogen (UK), while foetal bovine serum (FBS) was from Biosera (UK). Formaldehyde was bought from Fisher Scientific (USA). Protease inhibitor cocktail was purchased from Roche (UK). Sunitinib malate and 2,3,5-triphenyltetrazolium chloride (TTC) was purchased from Sigma Aldrich (USA) and IB-MECA was purchased from Tocris Bioscience (UK). Antibodies anti-PKCα (phospho T497) pPKCα and total PKC were from Abcam (UK), while glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (14C10) rabbit mAb and anti-rabbit linked IgG antibody conjugated to horseradish peroxidase were from Cell Signalling (UK). RNAlater, Ambion mirVana miRNA Isolation Kit, Applied Biosystems MicroRNA Reverse Transcription Kit, TaqMan Universal PCR Master Mix II (uracil N-Glycosylase not included), Applied Biosystems primers assays (U6, hsa-miR155, hsa-miR-15a, hsa-miR-16-1, rno-miR-1, hsa-miR-27a, hsa-miR-133a and hsa-miR-133b) were purchased from Life Technologies (USA).

Sandhu H., Cooper S., Hussain A., Mee C, & Maddock H. (2017). Attenuation of Sunitinib-induced cardiotoxicity through the A3 adenosine receptor activation. European journal of pharmacology, 814.

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