Ff510 di02

Manufactured by IDEX Corporation

The FF510-Di02 is a lab equipment product manufactured by IDEX Corporation. It serves as a digital flow sensor, designed to measure fluid flow rates accurately. The product specifications and technical details are the primary focus of this description, without interpretation or extrapolation on its intended use.

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Lab products found in correlation

Ff01 452 45, by IDEX Corporation (1 mentions) Ff01 609 181, by IDEX Corporation (1 mentions) Dg10 1500, by Thorlabs (1 mentions) Orca 05g, by Hamamatsu Photonics (1 mentions) Et605 70m, by Chroma Technology (1 mentions) Tcs sp5 2, by Leica (1 mentions) Ti sapphire laser, by Spectra-Physics (1 mentions)

2 protocols using ff510 di02

Cell Morphology Imaging in Acute Brain Slices

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For screening cells morphology and checking cell localization in acute brain slices we used a widefield infrared illumination system. This consisted of an IR-LED source (M780L2, Thorlabs) installed at the rear port of a SliceScope Scientifica microscope, an orientable blocking element to create oblique illumination and a condenser focusing the light on the sample. IR light transmitted through the sample was collected with an IR antireflection coated water-immersion objective (Nikon NIR MRD07420 N40X/0.80W) and sent to an IR CCD (IR-1000, DAGE-MIT).
For a first control of ReaChR expression, we performed widefield fluorescence imaging with a system comprising 2 interchangeable LED sources (Thorlabs M470L2, for YFP and M565L3 for dTomato) filtered by 2 interchangeable bandwidth excitation filters (Semrock FF01-452/45 for YFP and F01-545/55-25 for dTomato) and coupled to a diffuser (DG10-1500, Thorlabs) and an achromatic lens (f = 30 mm, #LA1805 Thorlabs). Fluorescence was collected through a tube lens (f = 200 mm), separated from excitation light using a dichroic mirror (Semrock FF510-Di02 for YFP and FF580-FDi01 for dTomato) and detected by a CCD camera (Orca-05G, Hamamatsu) after passing through a visible bandwidth filter (Semrock FF01-609/181 for YFP and FF01-665/150-25 for dTomato).

Chaigneau E., Ronzitti E., Gajowa M.A., Soler-Llavina G.J., Tanese D., Brureau A.Y., Papagiakoumou E., Zeng H, & Emiliani V. (2016). Two-Photon Holographic Stimulation of ReaChR. Frontiers in Cellular Neuroscience, 10, 234.

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Multi-Photon Microscopy for Vascular Imaging

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A Leica TCS SP5 II upright resonant scanning multi-photon microscope with a Leica 25X, 0.95NA water immersion objective was used throughout the entire experiment (Leica Microsystems, Germany). A Ti:sapphire laser (Spectra Physics, Irvine, California) was tuned to 720 nm for two-photon excitation in the UV range.
All in vivo vascular images were captured at 0.68 μm isotropic resolution with 8 line averages. For each image, 512 × 512 pixels were collected every 280 msec with non-descanned detectors and an 8 kHz resonant line scanning mirror. The vasculature dye ANEPPS channel consists of two dichroic mirrors with cutoff wavelengths at 510 nm and 560 nm (Semrock FF510-Di02 and FF560-Di01, respectively) and a 70 nm wide band-pass filter centered at 605 nm (Chroma Technology, ET605/70M). A short-pass filter with a cutoff wavelength at 680 nm is used to block the excitation light for all imaging experiments.
The 4.0 μm microsphere image stacks were captured at 0.4 × 0.4 × 0.4 μm and at 50 × 50 × 50 nm isotropic resolution with 2 line averages using two dichroic mirrors with cutoff wavelengths at 450 nm and 650 nm (cover full emission range of microspheres). The PSF images from the 0.2 μm microsphere were scanned at 52 × 52 × 142 nm resolution.

Dao L., Glancy B., Lucotte B., Chang L.C., Balaban R.S, & Hsu L.Y. (2015). A Model-Based Approach for Microvasculature Structure Distortion Correction in Two-Photon Fluorescence Microscopy Images. Journal of microscopy, 260(2), 180-193.

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