Hrp linked anti rabbit and anti mouse igg antibody

The anti-GFP (ab290), p-Ser/Thr (ab17464), Histone H3 (ab198757), and Giantin (ab37266) antibodies were acquired from Abcam (Cambridge, Britain). The anti-phospho-H3-S10 (3377) and anti-phospho-H3-T3 (9714), EGFR (4267), CyclinB1 (12231), 4EBP1 (53H11), p-4EBP1 (2855), p-Tyr (9411), p-Thr (9386) antibodies, and the HRP-linked anti-rabbit and anti-mouse IgG antibody were from Cell signaling technology (MA, USA). The anti-GAPDH and β-tubulin antibodies were from Beijing TransGen Biotech (Beijing, China). The secondary antibodies and anti-fade Prolong Gold with DAPI were from Invitrogen (Carlsbad, CA, USA). The anti-STK16 and anti-FLAG M2 monoclonal antibody (F3165) were from Sigma. GlutaMAX supplement was from Gibco. Puromycin dihydrochloride was from Selleck. All PCR primers were synthesized and genes were sequenced by Sangon Biotech (Shanghai, China). A Membrane and Cytosol Protein Extraction Kit was purchased from Beyotime (Shanghai, China).

The autophagy antibody sampler kit, the antibodies for phospho-S6K (T389/412), S6K, AKT pan, phospho-AKT (Thr-308), phospho-AKT (Ser-473), mTOR, AMPK Antibody sampler kit, the HRP-linked anti-rabbit and anti-mouse IgG antibody were all from Cell signaling technology. The anti-GFP (sc-9996) antibodies were acquired from Santa Cruz. The anti-GAPDH, anti-β-Tubulin and anti-β-Actin antibodies were from Beijing TransGen Biotech (Beijing, China). Dynabeads Protein G was from NOVEX. The secondary antibodies and anti-fade prolong Gold with DAPI were from Molecular Probes. The anti-FLAG M2 monoclonal antibody (F3165), dopamine, chloroquine, NH₄Cl were from Sigma. Ethylamine was from J&K Chemical (Shanghai, China), catechol from Energy Chemical (Shanghai, China), propylamine and phenethylamine from Tokyo Chemical Industry. GlutaMAX supplement was from Gibco. Puromycin dehydrochloride was from Selleck. Bafilomycin A1 was from Cayman. The siRNAs were ordered from GenePharma (Shanghai, China).

Mouse anti-CNPase, rabbit anti-Hes1, and rabbit anti-Hes5 antibodies were purchased from Abcam Corporation (Cambridge, UK). Anti-MBP antibodys were obtained from Millipore Corporation (Billerica, MA) for immune staining or ABcam (ab62631) for Western blot, and immobilon Western chemiluminescent HRP substrate were obtained from Millipore Corporation. Mouse anti-β-actin monoclonal antibody was purchased from CWBIO Corporation (Beijing, China). HRP-linked anti-rabbit and anti-mouse IgG antibodies were from Cell Signaling Corporation (Beverly, MA). Alexa Fluor 594 Donkey anti-rat IgG was from Invitrogen Corporation (San Diego, CA), and primers of Notch1, Notch2, Notch4, Hes1, and Hes5 were synthesized by TaKaRa Corporation (Seta, Japan). PrimeScript RT reagent Kit with gDNA Eraser, SYBR Premix Ex Taq (TliRNaseH Plus), and EASY Dilution were also from TaKaRa Corporation. Cuprizone (bis-cyclohexanoneoxalydihydrazone, CPZ) was purchased from Sigma Corporation (Ronkonkoma, NY). Quetiapine was a generous gift from Professor Li Xinmin, University of Manitoba, Canada. Halothane was obtained from Halocarbon Laboratories (Kinderkamack, NY). Biodentine dental cement was obtained from Septodont Corporation (Saint Maur des Fossés, France). DMSO was obtained from Sigma Corporation, and MW167, a γ-secretase II inhibitor, was purchased from Calbiochem Corporation (La Jolla, CA).