Mouse tnf elisa kit

Manufactured by BD

Sourced in United States

The Mouse TNF ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse tumor necrosis factor (TNF) in cell culture supernatants, cell lysates, serum, and plasma.

Automatically generated - may contain errors

Lab products found in correlation

Mouse il 1β elisa kit, by BD (2 mentions) Anti mouse il 1β af 401 na, by R&D Systems (1 mentions) Anti mouse caspase 1 p20 ag 20b 0042, by Adipogen (1 mentions) Anti mouse nlrp3 ag 20b 0014, by Adipogen (1 mentions) Anti flag f1804, by Merck Group (1 mentions) Poly da dt, by InvivoGen (1 mentions) Anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Tmb substrate reagent set, by BD (1 mentions) Mouse ifn γ elisa kit, by BD (1 mentions) Verikine mouse ifn alpha elisa kit, by PBL Assay Science (1 mentions) Elisa reader, by Molecular Devices (1 mentions) Mouse il 6 elisa kit, by BD (1 mentions)

5 protocols using mouse tnf elisa kit

Inflammasome Activation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

LPS (O111:B4, L2630), ATP (A7699), disuccinimidyl suberate (S1885), nigericin (N7143), and phorbol myristate acetate (PMA, P1585) were from Sigma-Aldrich; alum (77161) and the c-Myc tag (MA1-980) were from Thermo Fisher Scientific; and poly(dA:dT) was from InvivoGen. The following antibodies were used: anti-Flag (F1804) (Sigma); anti-mouse caspase-1 p20 (AG-20B-0042) and anti-mouse NLRP3 (AG-20B-0014) (AdipoGen); anti-human caspase-1 (ab108362) and anti-BRCC36 (ab108411) (Abcam); anti-mouse IL-1β (AF-401-NA) (R&D); anti-HA-tag (3724) (Cell Signaling Technology); and anti-VDR (sc-13133), anti-ASC (sc-514414), and anti-β-actin (sc-47778) (Santa Cruz). The Mouse TNF ELISA Kit (558534), Mouse IL-1β ELISA Kit (559603), and TMB Substrate Reagent Set (555214) were from BD Biosciences. The mouse IL-18 ELISA set (EMC06) was from ExCell Biotech (Table 1).

Rao Z., Chen X., Wu J., Xiao M., Zhang J., Wang B., Fang L., Zhang H., Wang X., Yang S, & Chen Y. (2019). Vitamin D Receptor Inhibits NLRP3 Activation by Impeding Its BRCC3-Mediated Deubiquitination. Frontiers in Immunology, 10, 2783.

+ Open protocol

+ Expand

Quantification of Cytokine Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Supernatants of cell culture, serum and ascites were stored in −70 °C freezer. IL-27, IFN-γ and TNF were detected by mouse IL-27 ELISA kit (Catalog#: 88-7274, eBioscience), mouse IFN-γ ELISA kit (Catalog#: 555138, BD Biosciences) and mouse TNF ELISA kit (Catalog#: 555268, BD Biosciences) according to the manufacturer’s instructions. Concentrations were calculated by regression analysis of a standard curve.

Wang Q., Ning H., Peng H., Wei L., Hou R., Hoft D.F, & Liu J. (2017). Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses. Nature Communications, 8, 867.

+ Open protocol

+ Expand

Mabc Infection and OMS Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

PMs (1.5 × 10⁵ cells) were cultured in 48-well plate and infected with Mabc (MOI of 1) with or without OMS treatment (5 μM). Cell supernatants were then collected after 3–18 h to measure TNF using the Mouse TNF ELISA kit (558534; BD Biosciences) according to the manufacturer’s protocol.

Jeon S.M., Kim Y.J., Nguyen T.Q., Cui J., Thi Bich Hanh B., Silwal P., Kim J.K., Kim J.M., Oh D.C., Jang J, & Jo E.K. (2022). Ohmyungsamycin promotes M1-like inflammatory responses to enhance host defence against Mycobacteroides abscessus infections. Virulence, 13(1), 1966-1984.

+ Open protocol

+ Expand

Cytokine Measurement Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

CCL3 ELISA was performed using the mouse CCL3/MIP-1α DuoSet (R&D Systems), TNF-α ELISA was performed using the mouse TNF ELISA kit (BD OptEIA), and IFN-α ELISA was performed using the Verikine Mouse IFN Alpha ELISA Kit (PBL Assay Science) according to the manufacturer’s instructions.

Uccellini M.B., Bardina S.V., Sánchez-Aparicio M.T., White K.M., Hou Y.J., Lim J.K, & García-Sastre A. (2020). Passenger Mutations Confound Phenotypes of SARM1-Deficient Mice. Cell reports, 31(1), 107498.

+ Open protocol

+ Expand

Measurement of Inflammatory Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The concentrations of interleukin (IL)-6, tumor necrosis factor (TNF), and IL-1β in the supernatant of homogenized tongue, and serum samples were measured using a mouse IL-6 ELISA kit, mouse TNF ELISA kit, and mouse IL-1β ELISA kit (all from BD Bioscience, NJ, USA), respectively, according to the manufacturer’s instructions. The cytokine levels were estimated by interpolation from a standard curve generated using an ELISA reader (Molecular Devices) at a wavelength of 450 nm.

Lee H.R., Yoo N., Kim J.H., Sohn K.Y., Kim H.J., Kim M.H., Han M.Y., Yoon S.Y, & Kim J.W. (2016). The Therapeutic Effect of PLAG against Oral Mucositis in Hamster and Mouse Model. Frontiers in Oncology, 6, 209.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!