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Fitc labeled annexin 5

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FITC-labeled annexin V is a fluorescent protein that binds to phosphatidylserine, a component of the cell membrane. It is used in cell biology research to detect apoptosis or programmed cell death.

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Lab products found in correlation

Accuri c6, by BD (1 mentions) Facstar cytofluorometer, by BD (1 mentions) Propidium iodide, by Abcam (1 mentions)

2 protocols using fitc labeled annexin 5

1

Annexin V Labeling of Eosinophils

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Classic annexin V labeling was used. Eosinophils were cultured in the presence of recombinant human IL-5 (30 ng/ml) (positive control) or ADP (10–100 nM) or MRS2395 (10 μM) for 24 h, followed by the addition of FITC-labeled annexin V (an early apoptosis marker) (1 μg/mL, Biovision, Mountain View, CA). The cells were incubated for 15 min on ice and immediately analyzed on the flow cytometer (BD Accuri C6, BD Biosciences).
Muniz V.S., Baptista-dos-Reis R., Benjamim C.F., Mata-Santos H.A., Pyrrho A.S., Strauch M.A., Melo P.A., Vicentino A.R., Silva-Paiva J., Bandeira-Melo C., Weller P.F., Figueiredo R.T, & Neves J.S. (2015). Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response. PLoS ONE, 10(10), e0139805.
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2

Analyzing Oncolytic Virus-Induced Apoptosis

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Cells infected with oncolytic vaccinia viruses and/or gemcitabine were trypsinized and washed once with complete medium. Aliquots of cells (5×105) were resuspended in 500 ml of binding buffer and stained with fluorescein isothiocyanate (FITC)-labeled Annexin V and propidium iodide (PI) (BioVision, Inc., Palo Alto, CA, USA) according to the manufacturer's instructions. Cell apoptosis and cell cycle were examined using FACS (FACStar cytofluorometer; BD Biosciences, San Jose, CA, USA).
Chen W., Fan W., Ru G., Huang F., Lu X., Zhang X., Mou X, & Wang S. (2018). Gemcitabine combined with an engineered oncolytic vaccinia virus exhibits a synergistic suppressive effect on the tumor growth of pancreatic cancer. Oncology Reports, 41(1), 67-76.
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