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Axiovert 50 microscope

Manufactured by Zeiss
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The Axiovert 50 is an inverted microscope designed for routine laboratory work. It features a sturdy, compact design and provides basic functionality for brightfield and phase contrast imaging. The microscope is equipped with objectives ranging from 2.5x to 100x magnification, allowing for a wide range of sample observation. The Axiovert 50 is a reliable and versatile tool for general microscopy applications.

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Axiocam hrm ccd camera, by Zeiss (1 mentions)

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Axiovert (339 citations) Axiovert microscope (338 citations)

2 protocols using axiovert 50 microscope

1

Sphere Formation Assay for CTCs

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CTCs and primary tumor cells were trypsinized and suspended in serum-free Neurobasal-A medium as single-cell suspensions, followed by seeding into 6-well plates at 5,000 cells per well. The cells were treated with or without 1 μM XAV939. After incubation for 8 days, the spheres were imaged and counted under an Axiovert 50 microscope (Zeiss) equipped with an AxioCam HRM CCD camera (Zeiss).
Liu T., Xu H., Huang M., Ma W., Saxena D., Lustig R.A., Alonso-Basanta M., Zhang Z., O’Rourke D.M., Zhang L., Gong Y., Kao G.D., Dorsey J.F, & Fan Y. (2018). Circulating glioma cells exhibit stem cell-like properties. Cancer research, 78(23), 6632-6642.
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2

Limiting Dilution Assay for CTCs

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In vitro limiting dilution assay was performed as previously described(18 (link)). Briefly, CTCs and primary tumor cells were trypsinized and suspended in serum-free Neurobasal-A medium as single-cell suspensions, followed by seeding into 96-well plates at 1, 5, 10, 20. 40, 80 cells/well. The cells were treated with or without 10 μM XAV939. After incubation for 10 days, the occurrence of spheres was observed under an Axiovert 50 microscope (Zeiss) and the wells with at least one sphere was recorded as positive.
Liu T., Xu H., Huang M., Ma W., Saxena D., Lustig R.A., Alonso-Basanta M., Zhang Z., O’Rourke D.M., Zhang L., Gong Y., Kao G.D., Dorsey J.F, & Fan Y. (2018). Circulating glioma cells exhibit stem cell-like properties. Cancer research, 78(23), 6632-6642.
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