In order to investigate the changes in substrate structural characteristics upon drying, the FTIR responses were conducted. The FTIR spectra were recorded by a Bruker INVENIO FTIR spectrometer with a Bruker Platinum ATR accessory. The generated spectra were analyzed using the OPUS software with 80 scans in a spectral range of 4000–400 cm−1, at 4 cm−1 resolution.
Invenio ftir spectrometer
Manufactured by Bruker
Sourced in Germany, United Kingdom
The Invenio FTIR spectrometer is a Fourier Transform Infrared (FTIR) spectrometer designed for laboratory use. It utilizes infrared spectroscopy to analyze the composition and structure of materials. The Invenio FTIR spectrometer can measure the absorption or emission characteristics of a sample within the infrared region of the electromagnetic spectrum.
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Lab products found in correlation
Platinum atr accessory, by Bruker (1 mentions)
Hts xt, by Bruker (1 mentions)
D8 advance powder, by Bruker (1 mentions)
Jsm 7200f, by JEOL (1 mentions)
Opus 7.5 software package, by Bruker (1 mentions)
Originpro 2018b software, by OriginLab (1 mentions)
Platinum atr, by Bruker (1 mentions)
Opus 7, by Bruker (1 mentions)
8 protocols using invenio ftir spectrometer
1
FTIR Analysis of Substrate Changes
2
High-throughput FTIR Spectroscopy of Bacterial Populations
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FTIR data were acquired from bulk populations following similar sample preparation and data collection protocols previously used by our group.16 (link) 20 μL aliquots from each sample with an optical density of 15 (OD600) was transferred to silicon substrates (Bruker Ltd, Coventry, UK) and dried in an oven (50 °C) prior data collection. FTIR data were acquired in the mid-IR range (4000–600 cm−1), with 64 spectral co-adds and 4 cm−1 resolution, using an accessory designed for high-throughput measurements (HTS-XT) coupled to a Bruker Invenio FTIR spectrometer. A total number of 16 FTIR spectra were collected from each bacterial strain including 4 spectra acquired from 4 analytical replicates.
3
FT-IR Spectroscopy of Compounds
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The IR absorbance spectra were recorded using the Bruker Invenio FT-IR spectrometer at room temperature. The resolution was set to 1 cm−1. A 200 μm path-length cell was used equipped with BaF2 windows. In total, 128 scans were recorded for each compound, corresponding to a measurement time of 2 min. The IR spectra were solvent corrected.
4
FTIR Spectral Analysis of Bulk Samples
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FTIR spectral data in the mid-IR range (4000–600 cm−1) were acquired from bulk populations using a Bruker Invenio FTIR spectrometer equipped with high-throughput measurements (HTS-XT) (Bruker Ltd., Coventry, UK) accessory. In total, 20 µL aliquots from each sample were transferred to a silicon substrate (Bruker Ltd., Coventry, UK) and dried for 30 min in an oven at 55 °C [3 (link)]. Each spectrum results from 64 accumulations with 4 cm−1 of spectral resolution. In total, 20 spectra were obtained for each experimental group from 5 analytical replicates per group (4 spectra per analytical replicate).
5
FTIR Analysis of Chemical Composition
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FTIR analysis was used for the identification of chemical groups and bonding present in the samples. The FTIR spectrum of all samples was analyzed using an Invenio FT-IR spectrometer (Bruker Optics, Germany). Each sample was ground and mixed thoroughly with potassium bromide at a ratio of 1:5 by mass of sample/KBr. Spectrum was recorded with a wavenumber range from 400 to 4000 cm−1, and 16 scans per sample according to the method of Kang et al. (2016).
6
FTIR Spectroscopy of Prepared Samples
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The test adopted the INVENIO FTIR spectrometer manufactured by
the Bruker company in Germany. 1 mg of the prepared sample was weighed
and dried . Then, it was placed in the sample chamber of the FTIR
spectrometer for testing. The test range was set as 400–4000
cm–1, heating rate was 5 °C·min–1, air flow was 120 mL·min–1, and cumulative
number of scans was 770.
the Bruker company in Germany. 1 mg of the prepared sample was weighed
and dried . Then, it was placed in the sample chamber of the FTIR
spectrometer for testing. The test range was set as 400–4000
cm–1, heating rate was 5 °C·min–1, air flow was 120 mL·min–1, and cumulative
number of scans was 770.
7
Comprehensive Nanocomposite Characterization
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Nanocomposite was characterized using XRD (Bruker D8 Advance powder diffractometer) to observe the crystal structure. SEM (Scanning electron microscope JSM-7200 F JEOL Japan) and EDX (INCA X Sight Oxford Instruments) analyzed the size and morphology of synthesized material. TGA (TGA/DSC 3+ Mettler Toledo Hong Kong) checked the thermal stability. UV (AQ7100APAC Thermofisher Scientific UK), FTIR (Invenio-FTIR Spectrometer Bruker, Germany), and zeta potential (Malvern zeta-analyzer) were employed to get further information on the prepared material.
8
Infrared Analysis of Dental Aligners
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The infrared analysis of the aligners was carried out with a Bruker Invenio FTIR spectrometer equipped with a Platinum ATR accessory for reflectance measurements (OPUS 7.5 software package, Bruker Optics, Ettlingen, Germany).
The ATR-FTIR spectra were collected at room temperature on dried samples just before the in vitro aging treatments (considered as a control group, Ctrl) and at the time points of 24 and 48 h. Five spectra were acquired in reflection mode in the spectral range 4000–600 cm−1 (spectral resolution 4 cm−1, 64 scans) on the external surface of each sample. A background spectrum was collected on the clean diamond crystal, before each sample measurement. All IR spectra were converted into Absorbance mode, interpolated in the spectral range 1800–700 cm−1, and two-points baseline linear fitted (OPUS 7.5 software, Bruker Optics). These preprocessed spectra were then subjected to principal component analysis (PCA; OriginPro 2018b software; OriginLab Corporation, Northampton, MA, USA) [25 (link)].
For each experimental group, height values of specific bands were calculated by using the Integration routine (K mode) (OPUS 7.5 software package, Bruker Optics, Ettlingen, Germany).
The ATR-FTIR spectra were collected at room temperature on dried samples just before the in vitro aging treatments (considered as a control group, Ctrl) and at the time points of 24 and 48 h. Five spectra were acquired in reflection mode in the spectral range 4000–600 cm−1 (spectral resolution 4 cm−1, 64 scans) on the external surface of each sample. A background spectrum was collected on the clean diamond crystal, before each sample measurement. All IR spectra were converted into Absorbance mode, interpolated in the spectral range 1800–700 cm−1, and two-points baseline linear fitted (OPUS 7.5 software, Bruker Optics). These preprocessed spectra were then subjected to principal component analysis (PCA; OriginPro 2018b software; OriginLab Corporation, Northampton, MA, USA) [25 (link)].
For each experimental group, height values of specific bands were calculated by using the Integration routine (K mode) (OPUS 7.5 software package, Bruker Optics, Ettlingen, Germany).
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