Heat inactivated foetal bovine serum

Human breast carcinoma cells (MDA-MB-231) were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). Mouse mammary carcinoma cells (4T1) were kindly provided by Dr. Jeong-Seok Nam (Gwangju Institute of Science and Technology, Gwangju, ROK). The cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) heat-inactivated foetal bovine serum (Cellgro, Manassas, VA, USA). TNFα was purchased from Sigma-Aldrich (St. Louis, MO, USA). The firefly and Renilla Dual-Glo^™ Luciferase Assay System was purchased from Promega (Madison, WI, USA). The pRL-null plasmid, which encodes Renilla luciferase, was also purchased from Promega.

Human breast carcinoma cells (MDA-MB-231, MDA-MB-435, T47D, and MCF7) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, MD, USA). Mouse mammary carcinoma cells (4 T1) were kindly provided by Dr. Jeong-Seok Nam (Gwangju Institute of Science and Technology, Gwangju, Korea). Cells were grown in Dulbecco’s modified Eagle’s medium (Corning Cellgro, Manassas, VA, USA) supplemented with 10 % (v/v) heat-inactivated foetal bovine serum (Corning Cellgro). TNFα, methanol, n-hexane, chloroform, ethyl acetate, n-butanol, dimethylsulfoxide (DMSO), Tris-base, HEPES, Triton X-100, glycerol, leupeptin, phenylmethylsulfonyl fluoride (PMSF) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). SDS, acrylamide, bisacrylamide, ammoniuk persulfate, nitrocellular membranes were from Bio-Rad Laboratories (Hercules, CA, USA). Pierce™ BCA Protein Assay Reagent was obtained from Thermo Scientific (Rockford, IL, USA).