Peg 35 kda

Unless otherwise noted, all polymer solutions are reported as percent weight solutions. Stock solutions of 30% BSA (Sigma-Aldrich), 20% PEG 35 kDa (Sigma-Aldrich), 10% PEO 200 kDa, 3% PEO 900 kDa, and 2.0% PEO 4,000 kDa (Dow Chemical) were dissolved in Dulbecco's Modified Eagle's Medium (DMEM; VWR). PEO solutions were centrifuged to sediment and remove silica particles introduced by the manufacturer. Concentrated stock solutions of BSA, PEG, and PEO were introduced to 96-well plates and mixed to form super-critical emulsions. Each super-critical concentration was then titrated with polymer-free culture medium until phase separation was no longer observed using methods similar to those reported previously for binodal determination in 96-well plates (Ruthven et al., 2017 (link)). The final BSA, PEG, and PEO concentrations were determined according to
where C_i refers to the initial concentration,V_i refers to the initial volume, C_f refers to the final concentration and V_f refers to the final volume. A Nikon Eclipse Ti microscope equipped with a 10x objective lens was used to observe microscopic emulsion characteristics.

DEX 20 kDa (Serva, Heidelberg, Germany), DEX 200 kDa (Serva, Heidelberg, Germany), DEX 450–650 kDa (Sigma-Aldrich, St. Louis, MO, USA), PEG 6 kDa (Serva, Heidelberg, Germany), PEG 15 kDa (Loba Chemi, Mumbai, India), PEG 20 kDa (Loba Chemi, Mumbai, India), PEG 35 kDa (Sigma-Aldrich, St. Louis, MO, USA).