Permount mounting solution

Manufactured by Thermo Fisher Scientific

Sourced in United States

Permount mounting solution is a clear, resin-based mounting medium used to permanently mount microscope specimens and tissue samples on glass slides. It provides a durable, transparent seal that protects the sample and allows for long-term storage and observation.

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Lab products found in correlation

Embedding center, by Leica camera (4 mentions) Harris hematoxylin solution, by Merck Group (2 mentions) Rotary microtome, by Leica camera (2 mentions) Eosin y, by Merck Group (2 mentions) Slide warmer, by Thermo Fisher Scientific (2 mentions) Microscopy, by Olympus (1 mentions) Triton x 100, by Thermo Fisher Scientific (1 mentions) Fitc conjugated goat anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions) Eclipse ti microscope, by Nikon (1 mentions) Eosin y solution, by Merck Group (1 mentions) Eosin solution, by Merck Group (1 mentions) Hematoxylin solution, by Merck Group (1 mentions)

5 protocols using permount mounting solution

Histological Analysis of Fixed Cerebral Cortex

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Fixed cerebral cortex tissues were washed with tap water, dehydrated with gradient ethyl
alcohol series from 70% to 100%, and cleaned with xylene. Tissues were embedded into
paraffin using Embedding center (Leica, Westlar, Germany) and made into paraffin tissue
blocks. Tissue blocks were sectioned 4 µm thick using a rotary microtome (Leica) and
sliced sections were placed on slide glass. Tissue slides were deparaffinized with xylene
and hydrated with ethyl alcohol series from 100% to 70%, and were kept in water. Tissue
slide were stained with Harris’ hematoxylin solution (Sigma-Aldrich) for 3 min, washed
with tap water for 10 min, reacted with Eosin Y (Sigma-Aldrich) for 1 min. After staining
process, tissue slides were dehydrated with gradient ethyl alcohol series, cleaned with
xylene, and sealed with a permount mounting solution (Fisher scientific, Fair Lawn, NJ,
USA). The morphological changes of the cerebral cortex were observed using Olympus
microscopy (Olympus, Tokyo, Japan) and microscopic images were taken.

GIM S.A., PARK D.J., KANG J.B., SHAH F.A, & KOH P.O. (2021). Identification of regulated proteins by resveratrol in glutamate-induced cortical injury of newborn rats. The Journal of Veterinary Medical Science, 83(4), 724-733.

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Formalin-Fixed Cell Immunocytochemistry

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Cells were fixed with 10% formalin and stained with Hematoxylin and eosin to examine the morphological changes in the cells in response to different treatments. For immunocytochemistry, the cells were fixed with 10% formalin and permeabilized with 0.1% Triton X-100 (Fisher Scientific, Pittsburgh, PA). The cells were incubated with primary antibody for 1 h at 37°C and then incubated with FITC-conjugated Goat anti mouse secondary antibody (Invitrogen, Carlsbad, CA) for 1 h at 37°C. The cells were mounted using Permount mounting solution (Fisher Scientific, Pittsburgh, PA). Fluorescence was detected using a fluorescence microscope Nikon Eclipse Ti microscope (Nikon Instruments Inc. Melville, NY).

Sarojini H., Billeter A.T., Eichenberger S., Druen D., Barnett R., Gardner S.A., Galbraith N.J., Polk HC J.r, & Chien S. (2017). Rapid tissue regeneration induced by intracellular ATP delivery—A preliminary mechanistic study. PLoS ONE, 12(4), e0174899.

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Paraffin-Embedded Tissue Sectioning and Staining

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Fixed brain tissues were washed with tap water for overnight, dehydrated by gradient of ethyl alcohol from 70 to 100%, and cleaned with xylene. Tissues were embedded with paraffin using embedding center (Leica, Wetzlar, Germany), sectioned into 4 μm coronal section on a rotary microtome (Leica), placed on slide glass, and dried on a slide warmer (Thermo Fisher Scientific, Waltham, MA, USA). Sections were depaffinized with xylene, rehydrated by gradient of ethyl alcohol from 100 to 70%, and stained with Harris’ hematoxylin solution (Sigma-Aldrich) and eosin Y solution (Sigma Aldrich). Stained sections were dehydrated by gradient of ethyl alcohol from 70 to 100%, cleaned with xylene, and mounted with permount mounting solution (Thermo Fisher Scientific). They were observed and photographed with Olympus microscope (Olympus, Tokyo, Japan).

Kang J.B., Park D.J., Shah M.A., Kim M.O, & Koh P.O. (2019). Lipopolysaccharide induces neuroglia activation and NF-κB activation in cerebral cortex of adult mice. Laboratory Animal Research, 35, 19.

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Tissue Preparation for Histological Analysis

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Fixed brain tissues were washed with flowing tap water, dehydrated by series of graded ethyl alcohol from 70 to 100%, and cleaned with xylene. Brain tissues were embedded in paraffin tank using embedding center (Leica, Westlar, Germany) and hardened as tissue blocks. Tissues were cut into 4 μm sections and placed on glass slides in tissue bath (Leica). Sections were dried on slide warmer (Thermo Fisher Scientific, Waltham, MA, USA), deparaffinized with xylene, and rehydrated by series of graded ethyl alcohol from 100 to 70%. They were stained with hematoxylin solution (Sigma-Aldrich) and eosin solution (Sigma-Aldrich), subsequently. Stained tissues were washed with tap water and dehydrated with series of graded ethyl alcohol. They were mounted with permount mounting solution (Thermo Fisher Scientific) and photographed using Olympus microscope (Olympus, Tokyo, Japan).

Park D.J., Kang J.B., Shah F.A, & Koh P.O. (2019). Resveratrol modulates the Akt/GSK-3β signaling pathway in a middle cerebral artery occlusion animal model. Laboratory Animal Research, 35, 18.

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Histological Processing of Brain Tissue

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Brain tissues were fixed in 4% formaldehyde with 0.1 M phosphate buffered saline) and
washed with tap water. Tissues were dehydrated by series of graded ethyl alcohol from 70
to 100%), cleaned with xylene, embedded in paraffin using embedding center (Leica,
Westlar, Germany). Paraffin blocks were cut into 4 µm sections and
sections were placed on glass slides. Sections were deparrafinized with xylene and
hydrated by graded with ethyl alcohol series from 100 to 70%. Sections were stained with
Harris’ hematoxylin solution (Sigma-Aldrich, St. Louis, MO, U.S.A.) for 3 min and Eosin Y
(Sigma-Aldrich) for 1 min. Sections were washed with tap water, dehydrated with graded
ethyl alcohol series, mounted with permount mounting solution (Thermo Fisher Scientific,
Waltham, MA, U.S.A.), photographed using Olympus microscope (Olympus, Tokyo, Japan).

PARK D.J., SHAH F.A, & KOH P.O. (2018). Quercetin attenuates neuronal cells damage in a middle cerebral artery occlusion animal model. The Journal of Veterinary Medical Science, 80(4), 676-683.

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