Ab174442

All the cells and supernatants were collected for ELISA. RIPA buffer (Applygen, China) was used to extract total protein. The protein concentration was determined using the bicinchoninic acid reagent (Thermo Fisher Scientific, USA). ELISA was then used to assess the levels of CXCL8 (ab174442; Abcam; USA) in the cytoplasm and supernatant according to the manufacturers’ instructions.

h‐JBMMSCs were cultured in osteoblast‐inducing conditional medium in 24‐well plates with or without APN. The cells in the APN‐treatment groups were cultured for 7 days with the addition of APN three times (once every 3 days). All the cells and supernatants were collected for ELISA. Total protein was extracted using the RIPA buffer (Applygen, Beijing, China), and the protein concentration was determined using the bicinchoninic acid reagent (Thermo Fisher Scientific, New York, NY, USA). ELISA was then used to assess the levels of CXCL1 (ab190805; Abcam, Cambridge, United Kingdom) and CXCL8 (ab174442; Abcam, Cambridge, United Kingdom) in the cytoplasm and supernatant according to the manufacturers’ instructions.