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Lncrna smart silencer nc

Manufactured by RiboBio
Sourced in China

The LncRNA Smart Silencer NC is a laboratory equipment designed for the manipulation of long non-coding RNA (lncRNA) expression. It functions as a silencing tool to selectively reduce the levels of target lncRNA molecules in cell cultures or experimental samples.

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2 protocols using lncrna smart silencer nc

1

Chemically Modified siRNA Knockdown

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Chemically modified small interfering RNAs (siRNAs) targeting CEMIP (SIGS0012573-1), β-catenin (SIGS0002532-1), TCF3 (SIGS0002560-1), WDR5 (SIGS0009833-1), h-HOXA11-AS Smart Silencer (lnc3161111110630), lncRNA Smart Silencer NC (lnc3N0000001-1-5), and negative control RNAs (siCont, siN0000001-1-5) were purchased from RiboBio Company (Guangzhou, China). The sequences of the above siRNAs are listed in Supplementary Table 2. RiboFECT™ CP Reagent (RiboBio, Guangzhou, China) was used to transfect siRNAs according to the manufacturer’s instructions.
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2

Silencing lncRNA-HOXB-AS1 in U251 and HS683 Cells

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U251 and HS683 cells in the logarithmic growth phase were incoculated into 6-well plates, after the cells grew to 50–60% fusion. The lncRNA-HOXB-AS1 smart silencer (a mixture containing three siRNAs and three antisense oligonucleotides) and its negative control, lncRNA smart silencer, NC, synthesized by RiboBio were used (Guangzhou, China). The cells were transfected using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, United States) following the manufacturer’s protocol.
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