The largest database of trusted experimental protocols

Quantikine hs human il 6 immunoassay kit

Manufactured by R&D Systems
Sourced in United States

The Quantikine® HS Human IL-6 Immunoassay Kit is a solid-phase ELISA designed to measure human interleukin 6 (IL-6) levels in cell culture supernates, serum, and plasma. It is a high-sensitivity assay with a minimum detectable dose typically less than 0.039 pg/mL.

Automatically generated - may contain errors

7 protocols using quantikine hs human il 6 immunoassay kit

1

Biomarker Quantification in Frozen Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols
sCD14, sCD163, and I-FABP were measured in frozen plasma specimens stored at −70°C using commercially available enzyme-linked immunosorbent assays (ELISA) [sCD14 and sCD163: Quantikine ELISA kit (R&D Systems, Minneapolis, MN) and I-FABP: ELISA kit (Hycult Biotech, Uden, Netherlands)]. CRP and IL-6 were measured in frozen sera stored at −70°C using ELISA [CRP: BNII nephelometer (Dade Behring, Deerfield, IL); IL-6: Quantikine HS Human IL-6 Immunoassay kit (R&D Systems)]. The limits of detection were as follows: sCD14 = 125 pg/mL, sCD163 = 0.613 ng/mL, I-FABP = 6.21 pg/mL, CRP = 0.17 mg/L, and hsIL-6 = 0.11 pg/mL. All tests were conducted according to manufacturers’ instructions. Coefficient variances (CVs) were <10% or were repeated. Sensitivities for these assays are listed on R&D Systems’ (the manufacturer’s) assay inserts and were not specifically examined. Positive controls included a quality control sample purchased from the manufacturer and included a high, medium, and low concentration of each analyte and were run in duplicate. The quality control catalogue numbers for each cytokine were as follows: sCD14 (#QC20), sCD163 (#QC61), I-FABP (#QC213), CRP (OQDB13), and hsIL-6 (#QC41).
+ Open protocol
+ Expand
2

Exercise-Induced Interleukin-6 Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-6 is a multifunctional cytokine involved in pro- as well as anti-inflammatory processes. Exercise-induced IL6 response is dependent on intensity and duration of the exercise [40 ]. The determination in serum was carried out at the study lab via Quantikine® HS Human IL-6 Immunoassay Kit (R&D Systems, Inc., MN, USA) pre-exercise and 3, 24, and 48 h post exercise.
+ Open protocol
+ Expand
3

Biomarkers of Immune Activation in HIV

Check if the same lab product or an alternative is used in the 5 most similar protocols
Using blood samples collected from WIHS and VAHH participants at the same time as their study visit and stored at −70 ° C, I-FABP, sCD14, and sCD163 were measured from frozen plasma and interleukin 6 (IL-6) from frozen sera. Commercially available enzyme-linked immunosorbent assays (ELISA) were used to measure I-FABP [ELISA kit (Hycult Biotech, Plymouth Meeting, Pennsylvania)]; sCD14 and sCD163 [Quantikine ELISA kit (R & D systems, Minneapolis, Minnesota, USA); and IL-6 [Quantikine HS Human IL-6 Immunoassay kit (R & D Systems, Minneapolis, Minnesota, USA)]. Samples from both cohorts were tested centrally at the same laboratories. Assays were performed in duplicate and in accordance with manufacturers' protocols.
+ Open protocol
+ Expand
4

Biomarker Assessment in Plasma Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma samples were aliquoted and stored at −20°C until subsequent analysis of the following biomarkers: high‐sensitivity C‐reactive protein (hsCRP), interleukin (IL)‐6, TNF‐α, and soluble CD14 (sCD14). The levels of hsCRP were determined with an immunoturbidimetric serum assay using Cobas 701 (Roche Diagnostics, Mannheim, Germany). Commercially available enzyme‐linked immunosorbent assays were used for the assay of IL‐6 (Quantikine HS Human IL‐6 immunoassay kit; R&D Systems, Minneapolis, MN), TNF‐α (using Quantikine HS Human TNF‐α immunoassay kit; R&D Systems), and sCD14 (Human CD14 ELISA Kit; Thermo Fisher Scientific, Waltham, MA) following the manufacturers' instructions.
+ Open protocol
+ Expand
5

Measuring IL-6 in Cohort Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The majority of blood samples were collected between 08:00 am and 10:00 am following a 12 h overnight fast in the EPITeen Cohort. IL-6 at 27 years was measured by the Luminex technology using the MILLIPLEX® Human High Sensitivity T Cell Panel in the Clinical Pathology Department of the São João Hospital Center, Porto. Females who were pregnant at the time were excluded from the assay. Sensitivity for MILLIPLEX® was 0•11 pg/ml.
In the Pelotas Cohort, non-fasting blood samples were taken from 08:00 am to 08:30 pm. The exclusion criterion for blood samples collecting was a current pregnancy. All samples were processed in the laboratory of the Epidemiological Research Center in the Federal University of Pelotas. Serum concentrations of IL-6 at 30 years were measured in duplicate by the Quantikine® HS Human IL-6 immunoassay kit (R&D Systems®, Inc.) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp). Sensitivity for Quantikine HS ELISA from 0•016 to 0•110 pg/ml.
+ Open protocol
+ Expand
6

Measurement of IL-6 and CRP in Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols
Non-fasting blood samples were drawn by cubital vein venipuncture. IL-6 was measured in pg/L by the Quantikine® HS Human IL-6 immunoassay kit (R&D Systems®, Inc.; Minneapolis, USA), while CRP was measured by immunoturdimetric assay (Labtest Diagnóstica SA, Minas Gerais, Brazil) in mg/L. Intra-assay and interassay coefficients of variation were 9.1% and 13.2%, respectively. Because CRP and IL-6 concentrations were non-normally distributed, we conducted non-paranormal transformation on IL-6 and CRP for all analyses (Fried et al., 2019) (link).
+ Open protocol
+ Expand
7

Biomarkers of Inflammation and Adiposity

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-6 and CRP concentrations at 18 years were assessed in non-fasting blood samples collected by venepuncture, using vacutainer tubes. All samples were processed in the laboratory located at the research center, stored in the same freezers at ultra-low temperature and registered in a central bio-repository. IL-6 (pg/mL) was measured using the Quantikine® HS Human IL-6 immunoassay kit (R&D Systems®, Inc.; Minneapolis, MN55413, USA) and CRP (mg/L) was measured by immunoturbidimetric assay (Labtest Diagnóstica SA, Minas Gerais, Brazil). Given IL-6 and CRP were asymmetrically distributed, they were log-transformed to obtain normalized residuals. These were divided into terciles for analysis when CRP and IL-6 were assessed as exposures.
Adiposity BMI (kg/m 2 ) at 18 years was used as a measure of adiposity. Height was measured twice, using an aluminum stadiometer with accuracy of 1mm, and the mean of the two measurements was used. Weight was measured with an accuracy of 10g, using an electronic scale connected to BodPod®. BMI was classified into underweight (BMI<18.5 kg/m 2 ), normal weight (BMI: 18.5 to 24.9 kg/m 2 ), overweight (BMI: 25 to 29.9 kg/m 2 ) and obesity (BMI≥30 kg/m 2 ) for analyses in which BMI was the exposure.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!