Quantikine hs human il 6 immunoassay kit

The majority of blood samples were collected between 08:00 am and 10:00 am following a 12 h overnight fast in the EPITeen Cohort. IL-6 at 27 years was measured by the Luminex technology using the MILLIPLEX® Human High Sensitivity T Cell Panel in the Clinical Pathology Department of the São João Hospital Center, Porto. Females who were pregnant at the time were excluded from the assay. Sensitivity for MILLIPLEX® was 0•11 pg/ml.
In the Pelotas Cohort, non-fasting blood samples were taken from 08:00 am to 08:30 pm. The exclusion criterion for blood samples collecting was a current pregnancy. All samples were processed in the laboratory of the Epidemiological Research Center in the Federal University of Pelotas. Serum concentrations of IL-6 at 30 years were measured in duplicate by the Quantikine® HS Human IL-6 immunoassay kit (R&D Systems®, Inc.) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp). Sensitivity for Quantikine HS ELISA from 0•016 to 0•110 pg/ml.

Non-fasting blood samples were drawn by cubital vein venipuncture. IL-6 was measured in pg/L by the Quantikine® HS Human IL-6 immunoassay kit (R&D Systems®, Inc.; Minneapolis, USA), while CRP was measured by immunoturdimetric assay (Labtest Diagnóstica SA, Minas Gerais, Brazil) in mg/L. Intra-assay and interassay coefficients of variation were 9.1% and 13.2%, respectively. Because CRP and IL-6 concentrations were non-normally distributed, we conducted non-paranormal transformation on IL-6 and CRP for all analyses (Fried et al., 2019) (link).

IL-6 and CRP concentrations at 18 years were assessed in non-fasting blood samples collected by venepuncture, using vacutainer tubes. All samples were processed in the laboratory located at the research center, stored in the same freezers at ultra-low temperature and registered in a central bio-repository. IL-6 (pg/mL) was measured using the Quantikine® HS Human IL-6 immunoassay kit (R&D Systems®, Inc.; Minneapolis, MN55413, USA) and CRP (mg/L) was measured by immunoturbidimetric assay (Labtest Diagnóstica SA, Minas Gerais, Brazil). Given IL-6 and CRP were asymmetrically distributed, they were log-transformed to obtain normalized residuals. These were divided into terciles for analysis when CRP and IL-6 were assessed as exposures.
Adiposity BMI (kg/m 2 ) at 18 years was used as a measure of adiposity. Height was measured twice, using an aluminum stadiometer with accuracy of 1mm, and the mean of the two measurements was used. Weight was measured with an accuracy of 10g, using an electronic scale connected to BodPod®. BMI was classified into underweight (BMI<18.5 kg/m 2 ), normal weight (BMI: 18.5 to 24.9 kg/m 2 ), overweight (BMI: 25 to 29.9 kg/m 2 ) and obesity (BMI≥30 kg/m 2 ) for analyses in which BMI was the exposure.