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Zorbax eclipse plus c8

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The Zorbax Eclipse Plus C8 is a reversed-phase high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. It features a spherical, fully porous silica-based stationary phase with a C8 functional group, providing a well-characterized and reliable stationary phase for HPLC applications.

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Lab products found in correlation

1290 infinity 2, by Agilent Technologies (2 mentions) Cpa225d, by Sartorius (1 mentions) Nexera x2 uplc system, by Shimadzu (1 mentions)

Spelling variants of this product

ZORBAX Eclipse Plus (42 citations) Eclipse Plus (30 citations) Zorbax Eclipse Plus C8 column (9 citations) Eclipse Plus C8 (4 citations) Zorbax Eclipse Plus C8 Rapid resolution HT column (2 citations)

4 protocols using zorbax eclipse plus c8

1

Agilent HPLC System for Chromatographic Analysis

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Agilent 1290 infinity chromatographic system comprises auto sampler with maximum 20 μL injection volume, diode array detector for UV detection, and quaternary pump for pumping solvent through Agilent Zorbax Eclipse Plus C8 (50 mm × 2.1 mm, 1.8 μm) column. This system is operated via OpenLAB ChemStation C.01.05 software. Sonicator (3510 Branson, UK). Electronic Balance (CPA225D Sartorius, Italy). pH meter (3505 Jenway, UK).
Mohamed H.M., Zaazaa H.E., Abdelkawy M, & Tantawy M.A. (2023). Exploiting the power of UPLC in separation and simultaneous determination of pholcodine, guaiacol along with three specified guaiacol impurities. BMC Chemistry, 17(1), 35.
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2

UHPLC-QTOF Metabolomics Analysis Protocol

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All prepared study samples, blanks, and controls were analyzed via LCMS using an Agilent 1290 Infinity II ultra-high-performance liquid chromatograph (UHPLC) coupled to an Agilent 6546 high-resolution quadrupole time-of-flight (Q-TOF) mass spectrometer with a dual AJS electrospray ionization source. An Agilent Zorbax Eclipse Plus C8 (2.1 × 100 mm, 1.8 μm) UHPLC column was used for compound separation. Each sample was first analyzed in full scan mode (MS1) with triplicate injections (2 μL) in each ionization mode. A fully randomized run sequence was used, which included replicates of solvent blanks, method blanks, and pooled QC samples. Data-dependent acquisition MS2 (DDA) was also performed on each sample extract in both positive and negative ionization modes, cycling between collision energies (CE) of 10, 20, and 40 eV. Full MS1 and MS2 method details can be found in Supplementary Methods Section 1. This data is available at the NIH Common Fund’s National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org (Study ID: ST002151, https://doi.org/10.21228/M8DD7D).
Chao A., Grossman J., Carberry C., Lai Y., Williams A.J., Minucci J.M., Purucker S.T., Szilagyi J., Lu K., Boggess K., Fry R.C., Sobus J.R, & Rager J.E. (2022). Integrative exposomic, transcriptomic, epigenomic analyses of human placental samples links understudied chemicals to preeclampsia. Environment international, 167, 107385.
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3

UHPLC-QTOF Metabolomics Analysis Protocol

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All prepared study samples, blanks, and controls were analyzed via LCMS using an Agilent 1290 Infinity II ultra-high-performance liquid chromatograph (UHPLC) coupled to an Agilent 6546 high-resolution quadrupole time-of-flight (Q-TOF) mass spectrometer with a dual AJS electrospray ionization source. An Agilent Zorbax Eclipse Plus C8 (2.1 × 100 mm, 1.8 μm) UHPLC column was used for compound separation. Each sample was first analyzed in full scan mode (MS1) with triplicate injections (2 μL) in each ionization mode. A fully randomized run sequence was used, which included replicates of solvent blanks, method blanks, and pooled QC samples. Data-dependent acquisition MS2 (DDA) was also performed on each sample extract in both positive and negative ionization modes, cycling between collision energies (CE) of 10, 20, and 40 eV. Full MS1 and MS2 method details can be found in Supplementary Methods Section 1. This data is available at the NIH Common Fund’s National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org (Study ID: ST002151, https://doi.org/10.21228/M8DD7D).
Chao A., Grossman J., Carberry C., Lai Y., Williams A.J., Minucci J.M., Purucker S.T., Szilagyi J., Lu K., Boggess K., Fry R.C., Sobus J.R, & Rager J.E. (2022). Integrative exposomic, transcriptomic, epigenomic analyses of human placental samples links understudied chemicals to preeclampsia. Environment international, 167, 107385.
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4

UPLC-MS/MS Quantification of Hormonal Contraceptives

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Chromatographic separation of ETO, LNG, MPA, NET, and their respective internal standards, was achieved using a Zorbax Eclipse Plus C8, 50 × 2.1 mm column with a 3.5 μm particle size (Agilent Technologies, Santa Clara, CA) on a Shimadzu Nexera X2 UPLC system (Shimadzu, Kyoto, Japan); a Zorbax Eclipse Plus C8, 4.6 × 12.5 mm guard column was also used for in-line sample clean-up. The mobile phase system used for separation consisted of 0.1% ammonium hydroxide in water (mobile phase A) and 0.1% ammonium hydroxide in methanol (mobile phase B). Analytes were eluted over a gradient from 50% to 80% mobile phase B over 5.00 min with a constant flow rate of 0.6 mL/min; ETO, LNG, MPA, and NET were eluted at 3.16, 3.06, 3.40, and 2.73 min, respectively. Quantification of hormonal contraceptives was performed using an API 6500 triple quadrupole mass analyzer (SCIEX, Redwood City, CA) using an ESI source operated in positive ionization and selective reaction monitoring (SRM) modes. Ion transitions monitored were as follows: ETO, 325.2→257.2 m/z; ETO-IS, 332.3→263.2 m/z; LNG, 313.2→245.1 m/z; LNG-IS, 320.3→251.1 m/z; MPA, 387.2→327.3 m/z; MPA-IS, 393.2→330.3 m/z; NET, 299.3→231.1 m/z; NET-IS, 306.3→237.1 m/z.
Knezevic C.E., Parsons T.L., Gollings R., Pandey A, & Marzinke M.A. (2023). Development and Validation of a Multiplexed Assay for the Measurement of Long-Acting Hormonal Contraceptives in Plasma via Liquid Chromatography-Tandem Mass Spectrometry. Journal of pharmaceutical and biomedical analysis, 228, 115321.
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