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3200 qtrap

Manufactured by Shimadzu
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The Shimadzu 3200 QTRAP is a triple quadrupole mass spectrometer designed for analytical testing and research applications. It features high sensitivity and selectivity, enabling accurate quantification and identification of target analytes in complex matrices. The instrument utilizes advanced ion optics and a robust collision cell to provide reliable performance across a wide range of applications.

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Lab products found in correlation

Scl hplc system, by Shimadzu (3 mentions) 4000 qtrap, by Shimadzu (2 mentions) N benzylbenzamide, by Merck Group (2 mentions) Xdb c18 column, by Agilent Technologies (2 mentions) Prominence lc, by Shimadzu (2 mentions) Kinetex c18, by Phenomenex (1 mentions) Lc 20ad, by Shimadzu (1 mentions) Discovery hs c18, by Merck Group (1 mentions)

6 protocols using 3200 qtrap

1

Quantitative Analysis of Nicotine and Metabolites

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Instrumental analyses were performed using an Applied Biosystems (3200 Qtrap) and Shimadzu SCL HPLC system controlled by Analyst 1.4.2 software with turbo V source for TurbolonSpray. A 3 mm by 50 mm, 5 micron Hypersil Gold system (Thermo Scientific, Waltham, MA, USA) was used for chromatographic separation. The mobile phase, maintained at a flow rate of 0.5 mL/min, consisted of 10 mM ammonium folate and methanol at a ratio of 10:90 (V/V). A positive multiple reaction monitoring (MRM) acquisition mode was utilized; with monitored ion transitions as follows: nicotine (163 > 130; 163 > 117), nicotine-d4 (167 > 134), cotinine (177 > 80; 177 > 98), and cotinine-d3 (180 > 80). Time set for each chromatographic separation was 2 min. Based on a linear regression generated from peak area ratios of each drug to its deuterated internal standard, a calibration curve (12.5 ng/mL –500 ng/mL) was designed for each compound. The internal standard for 3-hydroxycotinine was Cotinine-d3.
Akinola L.S., Mckiver B., Toma W., Zhu A.Z., Tyndale R.F., Kumar V, & Damaj M.I. (2019). C57BL/6 Substrain Differences in Pharmacological Effects after Acute and Repeated Nicotine Administration. Brain Sciences, 9(10), 244.
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2

Serum Lipid Profiling by LC-MS/MS

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We used the analysis method as reported by MacDonald et al.31 (link) Briefly, serum lipids were extracted with dichloromethane:methanol (1:1) containing butylated hydroxytoluene (50 μg/mL). The lipid fractions were hydrolyzed with 0.5 N potassium hydroxide for 1.5 hours at 35°C and subjected to an aminopropyl solid‐phase extraction. The eluted lipid samples were analyzed with LC‐MS/MS (Applied Biosystems 3200 Qtrap with Shimadzu LC20AD) with a reverse‐phase column (Kinetex C18 HPLC column; Phenomenex) using an acetonitrile/2‐propanol/5 mmol/L ammonium acetate gradient.
Miyazaki‐Anzai S., Masuda M., Demos‐Davies K.M., Keenan A.L., Saunders S.J., Masuda R., Jablonski K., Cavasin M.A., Kendrick J., Chonchol M., McKinsey T.A., Levi M, & Miyazaki M. (2014). Endoplasmic Reticulum Stress Effector CCAAT/Enhancer‐binding Protein Homologous Protein (CHOP) Regulates Chronic Kidney Disease–Induced Vascular Calcification. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 3(3), e000949.
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3

Quantifying Org27569 Levels in Blood and Brain

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Org27569 was administered either intraperitoneally (30mg/kg) or intracerebroventricularly (100 μg) and levels of the drug were quantified, respectively, in the blood and brain, or brain, according to established methods (Kinsey et al., 2009 (link)), using the HPLC/MS/MS method with an Applied Bio systems (Carlsbad, CA, USA) 3200 Q trap with a turbo V source for TurboIonSpray attached to a Shimadzu SCL HPLC system (Kyoto, Japan). Chromatographic separation was performed using a Discovery HS C18, 2.1×150mm, 3 μm column (Supelco, St. Louis, MO, USA).
Gamage T.F., Ignatowska-Jankowska B.M., Wiley J.L., Abdelrahman M., Trembleau L., Greig I.R., Thakur G.A., Tichkule R., Poklis J., Ross R.A., Pertwee R.G, & Lichtman A.H. (2014). In-vivo pharmacological evaluation of the CB1-receptor allosteric modulator Org-27569. Behavioural pharmacology, 25(2), 182-185.
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4

Quantification of Nicotine by LC-MS/MS

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The LC/MS/MS system used was an Applied Bio systems 3200 Qtrap with a turbo V source for TurbolonSpray with a Shimadzu SCL HPLC system controlled by Analyst 1.4.2 software. The chromatographic separation was performed using an Hypersil Gold, 3 mm X 50 mm, 5 micron (Thermo Scientific, USA). The mobile phase contained 10 mM ammonium formate; methanol (10:90 V/V) and was delivered at a flow rate of 0.5 ml/min. The acquisition mode used was multiple reaction monitoring (MRM) in a positive mode. Transition ions monitored for nicotine (163>130; 163>117) and nicotine-d4 (167>134). The total chromatographic separation time for each extract injection was 2 min. A calibration curve ranging from 12.5 ng/ml to 500 ng/ml was constructed for each compound based on linear regression using the peak area ratios of the drug to its deuterated internal standard.
Alsharari S.D., King J.R., Nordman J.C., Muldoon P.P., Jackson A., Zhu A.Z., Tyndale R.F., Kabbani N, & Damaj M.I. (2015). Effects of Menthol on Nicotine Pharmacokinetic, Pharmacology and Dependence in Mice. PLoS ONE, 10(9), e0137070.
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5

Quantitative LC-MS/MS Bioanalysis of Compound 13

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Levels of analog 13 for in vitro and in vivo pharmacology assays were monitored by LC-MS/MS using an AB Sciex (Framingham, MA) 3200 Q.TRAP® or 4000 Q.TRAP® mass spectrometer coupled to a Shimadzu (Columbia, MD) Prominence LC. The compound was detected with the mass spectrometer in positive MRM (multiple reaction monitoring) mode by following the precursor to fragment ion transition 470.1/360.1. The cleanest results were obtained using the parent mass composed of a 35Cl and a 37Cl (m+1). An Agilent C18 XDB column (5 micron, 50 X 4.6 mm) was used for chromatography with the following conditions: Buffer A: dH20 + 0.1% formic acid + 2 mM NH4 acetate, Buffer B: methanol + 0.1% formic acid + 2 mM NH4 acetate, 0 - 1.5 min 3% B, 1.5 – 2.5 min gradient to 100% B, 2.5 - 3.5 min 100% B, 3.5 - 3.6 min gradient to 3% B, 3.6 - 4.5 3% B. N-benzylbenzamide (transition 212.1 to 91.1 from Sigma (St. Louis, MO) was used as an internal standard (IS).
Nguyen T.P., Wang W., Sternisha A.C., Corley C.D., Wang H.Y., Wang X., Ortiz F., Lim S.K., Abdullah K.G., Parada L.F., Williams N.S., McBrayer S.K., McDonald J.G., De Brabander J.K, & Nijhawan D. (2023). Selective and brain-penetrant lanosterol synthase inhibitors target glioma stem-like cells by inducing 24(S),25-epoxycholesterol production. Cell chemical biology, 30(2), 214-229.e18.
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6

LC-MS/MS Quantitation of Pharmacological Compounds

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Compound levels for in vitro and in vivo pharmacology assays were monitored by LC-MS/MS using an AB Sciex (Framingham, MA) 3200 QTRAP® or 4000 QTRAP® mass spectrometer coupled to a Shimadzu (Columbia, MD) Prominence LC. Analytes were detected with the mass spectrometer in positive MRM (multiple reaction monitoring) mode by following the precursor to fragment ion transition for two daughter ions. Only one parent daughter pair, indicated here, was used for quantitation: 40: 368.100/283.000; 48: 385.300/300.000; 49: 399.123/314.1; 43: 369.300/257.500; 44: 369.300/258.000. An Agilent C18 XDB column (5 micron, 50 X 4.6 mm) was used for chromatography for 40 and 49 with the following conditions: Buffer A: dH20 + 0.1% formic acid, Buffer B: methanol + 0.1% formic acid, 0 - 1.5 min 3% B, 1.5 - 2 min gradient to 100% B, 2 - 3.5 min 100% B, 3.5 - 3.6 min gradient to 3% B, 3.6 - 4.5 3% B. N-benzylbenzamide (transition 212.1 to 91.1 from Sigma (St. Louis, MO) was used as an internal standard (IS). Evaluation of other compounds employed nearly identical conditions except the gradient utilized was 0 - 1.0 min 3% B, 1.0 – 2 min gradient to 100% B, 2.0- 3.5 min 100% B, 3.5 - 3.6 min gradient to 3% B, 3.6 - 4.5 3% B.
Hu B., Toda K., Wang X., Antczak M., Smith J., Geboers S., Nishikawa G., Li H., Dawson D., Fink S., Desai A., Williams N.S., Markowitz S.D, & Ready J.M. (2022). Orally bioavailable quinoxaline inhibitors of 15-prostaglandin dehydrogenase (15-PGDH) promote tissue repair and regeneration. Journal of medicinal chemistry, 65(22), 15327-15343.
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