Ferrous sulfate heptahydrate

Acetonitrile (HPLC grade), 95% ethanol, 37% hydrochloric acid, sodium hydroxide, sulfuric acid, and dichloromethane were purchased from RCI Labscan, Bangkok, Thailand. Gallic acid, Folin–Ciocalteau phenol reagent, aluminum chloride, sodium acetate trihydrate, ferric chloride solution, ferrous sulfate heptahydrate, and potassium persulfate sodium carbonate were obtained from Loba Chemie, Mumbai, India. A549 (ATCC^® CCL-185TM) human lung adenocarcinoma cell line was purchased from the American Type Culture Collections (ATCC; Manassas, VA, USA). HaCaT cell line was purchased from Pacific Science, Co., Ltd. (Bangkok, Thailand). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum, 100 U/mL and 100 µg/mL streptomycin, and trypsin-EDTA were purchased from Invitrogen (Carlsbad, CA, USA). Dragendorff’s reagent, phosphotungstic acid, Gallic acid, DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-Tris(2-pyridyl)-s-triazine, ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and sulforhodamine B (SRB) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Annexin V-FITC and PI solution were purchased from BD Biosciences, CA, USA). DCF-DA was purchased from Sigma Merck KGaA (Darmstadt, Germany).

Gallic acid, Griess reagent, DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-Tris(2-pyridyl)-s-triazine, ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and coumarin were purchased from Sigma-Aldrich, St. Louis, USA. Absolute ethanol, dimethyl sulfoxide, sodium bicarbonate, sodium nitrate, and sodium hydroxide were purchased from RCI Labscan, Bangkok, Thailand. The Folin–Ciocalteu phenol reagent, aluminum chloride, sodium acetate trihydrate, ferrous sulfate heptahydrate (99% purity), and potassium persulfate were obtained from Loba Chemie, Mumbai, India. Quercetin (98% purity), epigallocatechin (EGCG) (98% purity), ascorbyl glucoside, phenoxyethanol, and triethanolamine were purchased from Chanjao Longevity Co., Ltd., Bangkok, Thailand. Clindamycin and diclofenac diethylammonium were purchased from RPC International Co., Ltd., Bangkok, Thailand. Carbopol Ultrez-21 polymer, propylene glycol, and glycerin were purchased from Namsiang, Bangkok, Thailand. Iron (III) chloride hexahydrate and 37% hydrochloric acid were purchased from Qrec, New Zealand.

Gallic acid, Griess reagent, DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-Tris(2-pyridyl)-s-triazine, and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) were purchased from Sigma-Aldrich, St. Louis, USA. Absolute ethanol, dimethyl sulfoxide, sodium bicarbonate, sodium nitrate, acetic acid, and sodium hydroxide were purchased from RCI Labscan, Bangkok, Thailand. Iron (III) chloride hexahydrate and 37% hydrochloric acid were purchased from Qrec, New Zealand. The Folin–Ciocalteu phenol reagent, aluminum chloride, sodium acetate trihydrate, ferrous sulfate heptahydrate (99% purity), and potassium persulfate were obtained from Loba Chemie, Mumbai, India. Quercetin (98% purity), epigallocatechin (EGCG) (98% purity), ascorbyl glucoside, phosphatidylcholine, cholesterol, poloxamer 407, and polyurethane-62 were purchased from Chanjao Longevity Co., Ltd., Bangkok, Thailand. Dulbecco’s Modified Eagle’s Medium (DMEM) with high glucose, fetal bovine serum (FBS), penicillin-streptomycin, and trypsin-EDTA were purchased from Gibco (Waltham, MA, USA).