NSCLC cell lines, A549, and NCI-H358 (hereafter H358) were obtained from ATCC in 2014. The cells were grown in RPMI-1640 medium with 10% fetal bovine serum (FBS) and antibiotics-antimycotics (#15240-062, Invitrogen, Carlsbad, CA, USA), at 37 °C and 5% CO2, and controlled every month for mycoplasma contamination. Recombinant human TGF-β1 was from R&D Systems (Minneapolis, MN, USA). The inhibitors EPZ5676, SGC0946, PFI-1, and SAHA were purchased from Cayman (Ann Arbor, Michigan, USA). The antibodies are listed in Additional file 1 .
Sgc0946
Manufactured by Cayman Chemical
Sourced in United States
SGC0946 is a chemical compound that can be used as a research tool in various laboratory settings. It is a selective inhibitor of the enzyme bromodomain-containing protein 4 (BRD4). The core function of this product is to act as a chemical probe to study the biological role of BRD4 and its potential applications in research.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human tgf β1, by R&D Systems (1 mentions)
Antibiotics antimycotic, by Thermo Fisher Scientific (1 mentions)
Nci h358, by American Type Culture Collection (1 mentions)
Pfi 1, by Cayman Chemical (1 mentions)
Epz5676, by Cayman Chemical (1 mentions)
Sb431542, by Cayman Chemical (1 mentions)
Sodium butyrate nab, by Merck Group (1 mentions)
2 protocols using sgc0946
1
Investigating NSCLC Cell Line Responses
2
Sodium Butyrate and Small Molecule Inhibitors
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Sodium butyrate (NaB; Sigma-Aldrich) was used at a final concentration of 250 μM. SB431542 (purity: ≥98%; Cayman Chemical) was used at a final concentration of 2 μM. SGC0946 (purity: ≥98%; Cayman Chemical) was used at a final concentration of 3 μM.
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