20 nL of stock compounds solvated in DMSO were dispensed using an Echo Acoustic Liquid Handler instrument (Labcyte) before adding 750 293A-TEAD-LUC cells per well in white 1,536-well plates (Corning). 24 h later, 2 μL of ONE-glo luciferase detection reagent (Promega) was dispensed per well and luminance signal recorded with an Envision plate reader (Perkin Elmer).
One glo luciferase detection reagent
Manufactured by Promega
ONE-Glo Luciferase Assay System is a reagent used for the detection and quantification of luciferase reporter gene activity in cell-based assays. The reagent contains the necessary components for cell lysis and luciferase detection, providing a sensitive and reliable method for measuring gene expression.
Automatically generated - may contain errors
2 protocols using one glo luciferase detection reagent
1
High-throughput TEAD reporter assay
2
High-Throughput Luciferase Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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20 nL of stock compounds solvated in DMSO were dispensed using an Echo Acoustic Liquid Handler instrument (Labcyte, San Jose, CA) before adding 750 293A-TEAD-LUC cells per well in white 1536-well plates (Corning). 24 hr later, 2 µL of ONE-glo luciferase detection reagent (Promega, Madison, WI) was dispensed per well and the luminance signal was recorded with an Envision plate reader (Perkin Elmer, Waltham, MA).
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