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Pi 3 p dic8

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PI(3)P diC8 is a synthetic, water-soluble analog of phosphatidylinositol 3-phosphate (PI(3)P). It is a useful tool for studying PI(3)P-dependent signaling pathways in biological systems.

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Lab products found in correlation

Bafilomycin a1, by InvivoGen (2 mentions) 2 3 cgamp, by Biolog (2 mentions) 60mer dsdna, by DNA-Technology (2 mentions) 3 methyladenine 3 ma, by InvivoGen (2 mentions) Vps34 inhibitor 1, by Cayman Chemical (2 mentions) Vps34 in1, by Cayman Chemical (2 mentions) Brefeldin a bfa, by Merck Group (2 mentions) Pi 4 5 p2 dic8, by Echelon Biosciences (2 mentions) Bovine serum albumin (bsa), by New England Biolabs (1 mentions) Typhoon fla 7000, by GE Healthcare (1 mentions) Chloroquine, by Merck Group (1 mentions) Bafa1, by Merck Group (1 mentions) Oregon green 488 dextran, by Thermo Fisher Scientific (1 mentions) Apilimod, by MedChemExpress (1 mentions) Lysotracker blue dnd 22, by Thermo Fisher Scientific (1 mentions) Fura 2 am, by Thermo Fisher Scientific (1 mentions) Fura 2 acetoxymethyl ester, by Thermo Fisher Scientific (1 mentions) Lysosensor yellow blue dextran, by Thermo Fisher Scientific (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Pi 3 4 5 p3 dic8, by Echelon Biosciences (1 mentions)

5 protocols using pi 3 p dic8

1

Diverse Cell Lines and Viral Models for STING Pathway Research

Cited 1 time
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The cell lines used for the study were iBMDC-MycSTING, THP-1, Vero cells, HEK-293T cells, HaCaT, HeLa cells, and Human foreskin fibroblasts (HFF). The choice of cell models depended on the experiments; e.g. THP-1 cells express high levels of cytokines and were used to evaluate gene expression, while HeLa cells have a large cytoplasm and were often the cell line of choice for immunofluorescence. SAVI patient-derived fibroblasts cells were obtained as described previously14 (link). Human blood-derived monocytes were isolated from normal healthy blood donor buffy coats obtained from Aarhus University Hospital Blood Bank. Human primary fibroblasts were obtained from healthy donors from Department of Dermatology and Venereology, Aarhus University Hospital. The viruses used were HSV-1 (Strain F+, and McKrae), HSV-2 (strain 333), Sendai virus (strain Cantrell). The reagents used were 2’3’-cGAMP (BIOLOG), 60mer dsDNA (DNA technology), PI(3)P diC8 (Echelon), phorbol 12-myristate 13-acetate (PMA, Sigma), Brefeldin A (BFA, Sigma), Bafilomycin A1(BafA1, InvivoGen), 3-Methyladenine (3-MA, InvivoGen), VPS34 Inhibitor 1 (VPS34 IN1, Cayman).
Zhang B.C., Nandakumar R., Reinert L.S., Huang J., Laustsen A., Gao Z.L., Sun C.L., Jensen S.B., Troldborg A., Assil S., Berthelsen M.F., Scavenius C., Zhang Y., Windross S.J., Olagnier D., Prabakaran T., Bodda C., Narita R., Cai Y., Zhang C.G., Stenmark H., Doucet C.M., Noda T., Guo Z., Goldbach-Mansky R., Hartmann R., Chen Z.J., Enghild J.J., Bak R.O., Thomsen M.K, & Paludan S.R. (2020). STEEP mediates STING ER exit and activation of signaling. Nature immunology, 21(8), 868-879.
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2

Diverse Cell Lines and Viral Models for STING Pathway Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell lines used for the study were iBMDC-MycSTING, THP-1, Vero cells, HEK-293T cells, HaCaT, HeLa cells, and Human foreskin fibroblasts (HFF). The choice of cell models depended on the experiments; e.g. THP-1 cells express high levels of cytokines and were used to evaluate gene expression, while HeLa cells have a large cytoplasm and were often the cell line of choice for immunofluorescence. SAVI patient-derived fibroblasts cells were obtained as described previously14 (link). Human blood-derived monocytes were isolated from normal healthy blood donor buffy coats obtained from Aarhus University Hospital Blood Bank. Human primary fibroblasts were obtained from healthy donors from Department of Dermatology and Venereology, Aarhus University Hospital. The viruses used were HSV-1 (Strain F+, and McKrae), HSV-2 (strain 333), Sendai virus (strain Cantrell). The reagents used were 2’3’-cGAMP (BIOLOG), 60mer dsDNA (DNA technology), PI(3)P diC8 (Echelon), phorbol 12-myristate 13-acetate (PMA, Sigma), Brefeldin A (BFA, Sigma), Bafilomycin A1(BafA1, InvivoGen), 3-Methyladenine (3-MA, InvivoGen), VPS34 Inhibitor 1 (VPS34 IN1, Cayman).
Zhang B.C., Nandakumar R., Reinert L.S., Huang J., Laustsen A., Gao Z.L., Sun C.L., Jensen S.B., Troldborg A., Assil S., Berthelsen M.F., Scavenius C., Zhang Y., Windross S.J., Olagnier D., Prabakaran T., Bodda C., Narita R., Cai Y., Zhang C.G., Stenmark H., Doucet C.M., Noda T., Guo Z., Goldbach-Mansky R., Hartmann R., Chen Z.J., Enghild J.J., Bak R.O., Thomsen M.K, & Paludan S.R. (2020). STEEP mediates STING ER exit and activation of signaling. Nature immunology, 21(8), 868-879.
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3

BSA Glucosylation by LtpM and SetA

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2 μg of bovine serum albumin (BSA, New England Biolabs (Frankfurt, Germany)), as an artificial substrate, was incubated with recombinant LtpM or SetA and 10 μm UDP-[14C]glucose in a buffer containing 50 mm Hepes (pH 7.5), 3 mm MgCl2, 2 mm MnCl2, 10 mm DTT, and 1 μm PI3P diC8 (Echelon Biosciences) at 30 °C (if other conditions are not indicated). Total volume was 20 μl for the lysate glucosylation and 10 μl for the glucosylation of BSA. Labeled proteins were analyzed by SDS-PAGE followed by phosphorimaging (Typhoon FLA 7000, GE Healthcare, Freiburg, Germany).
Levanova N., Mattheis C., Carson D., To K.N., Jank T., Frankel G., Aktories K, & Schroeder G.N. (2018). The Legionella effector LtpM is a new type of phosphoinositide-activated glucosyltransferase. The Journal of Biological Chemistry, 294(8), 2862-2879.
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4

Lysosomal Trafficking and Acidity Assay

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Apilimod (MedChemExpress); WX8 (gift from Juan S. Bonifacino lab) Oregon Green 488-dextran 10,000 mW (OG; Thermo Fisher); chloroquine, BafA1, monensin, and nigericin (Sigma-Aldrich); LysoTracker Blue DND-22 (Invitrogen); Magic Red cathepsin B essay (ImmunoChemistry Technologies); PI(3,5)P2 diC8, PI(4,5)P2 diC8, PI3P diC8 (Echelon Biosciences).
Leray X., Hilton J.K., Nwangwu K., Becerril A., Mikusevic V., Fitzgerald G., Amin A., Weston M.R, & Mindell J.A. (2022). Tonic inhibition of the chloride/proton antiporter ClC-7 by PI(3,5)P2 is crucial for lysosomal pH maintenance. eLife, 11, e74136.
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5

Electrophysiology and Imaging Experiments using Lipid Ligands

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Chemicals used for electrophysiological and imaging experiments were purchased from Sigma-Aldrich, MERCK. The lipid ligands including phosphatidylinositol-3,5-bisphosphate- diC8 (PI(3,5)P2-diC8), PI(4,5)P2-diC8, PI(3)P-diC8, PI(5)P-diC8, and PI(3,4,5)P3-diC8 were all purchased from Echelon Biosciences. LysoTracker Red DND-99 (Thermo Fisher Scientific, L7528), LysoSensor Yellow/Blue Dextran (Invitrogen, L22460), TPC2-A1-N (MCE, HY-131614), trans Ned-19 (MCE, HY-103316A), YM201636 (MCE, HY-13228), Fura2 acetoxymethyl ester (Fura2 AM, F14185, Invitrogen), and Tetrandrine (MCE, HY-13764) were purchased as indicated in parentheses.
Wang Q., Wang Z., Wang Y., Qi Z., Bai D., Wang C., Chen Y., Xu W., Zhu X., Jeon J., Xiong J., Hao C., Zhu M.X., Wei A, & Li W. (2023). A gain-of-function TPC2 variant R210C increases affinity to PI(3,5)P2 and causes lysosome acidification and hypopigmentation. Nature Communications, 14, 226.
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