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Ifngr−/− is a laboratory mouse strain with a genetic deletion of the interferon gamma receptor (Ifngr) gene. This strain is a useful tool for studying the role of interferon gamma signaling in various biological processes.

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Lab products found in correlation

Lyz2 cre, by Jackson ImmunoResearch (2 mentions) Andcasp1 11, by Jackson ImmunoResearch (2 mentions) Cd11c cre, by Jackson ImmunoResearch (2 mentions) Raw264, by American Type Culture Collection (1 mentions) Vav1 cre, by Jackson ImmunoResearch (1 mentions)

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Ifngr−/− mice (3 citations) B6.129S7-Ifngr1tm1Agt/J (Ifngr−/−) mice (2 citations)

4 protocols using ifngr

1

Genetic Manipulation of Autophagy Genes

Cited 4 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Atg5f/f and
Atg5f/f-Lyz2cre+/−mice were generated as described previously in an enhanced barrier
facility17 (link),56 (link).
Becn1f/f-
Lyz2cre+/−57 (link),
Fip200f/f-
Lyz2cre+/−58 (link),
Atg7f/f-Lyz2cre+/−51 (link), and
Atg16l1f/f-
Lyz2cre+/− 59 (link) were generated in the same way as
Atg5f/f-
Lyz2cre+/−.
Becn1f/f-
CD11c-cre+/−, and
Becn1f/f-
Mrp8-cre+/− were generated by breeding
Becn1f/f to CD11c-
cre+/− (#007567) and
Mrp8-cre+/− (#021614) from the Jackson
Laboratory. Rag1−/− (#002216),
Ifngr−/− (#003288), and
Casp1/11−/− (#016621) mice were
from the Jackson Laboratory.
Rubicon−/− knockout mice were
kindly provided by Doug Green and Jennifer Martinez25 (link). All mice used for experimental
procedures were backcrossed in house to B6/J except for
Rubicon−/− mice. 8–12 weeks
of age and sex-matched littermates were used unless specified otherwise and were
subject to randomization. Statistical consideration was not used to determine
mouse sample sizes. Mice were housed and bred at Washington University in St.
Louis in specific pathogen-free conditions in accordance with federal and
university guidelines, and protocols were approved by the Animal Studies
Committee of Washington University.
Wang Y.T., Zaitsev K., Lu Q., Li S., Schaiff W.T., Kim K.W., Droit L., Wilen C.B., Desai C., Balce D.R., Orchard R.C., Orvedahl A., Park S., Kreamalmeyer D., Handley S.A., Pfeifer J.D., Baldridge M.T., Artyomov M.N., Stallings C.L, & Virgin H.W. (2020). Select autophagy genes maintain quiescence of tissue-resident macrophages and increase susceptibility to Listeria monocytogenes. Nature microbiology, 5(2), 272-281.
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2

Murine Cell Culture and Transgenic Mice

Cited 1 time
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Murine cell line Raw264.7 was purchased from ATCC. Cells were cultured in DMEM medium in a 5% CO2 incubator at 37°C, and subcultured every 2–3 days. Rag1−/−, Ifngr−/−, Ifng−/−, Prf1−/−, Vav1-Cre, and Rosa26-LSL-rtTA mice were purchased from Jackson Laboratory. Transgenic HIF1A-TPM mice were kindly provided by Professor James Bridge from Cincinnati Children’s Hospital Medical Center (CCHMC).22 (link) Mice, with the genotype of Ncr1-iCre, were kindly provided by Professor Eric Vivier from the French INSERM Laboratory.23 (link) LCMV-infected Prf1−/− mouse model and the repeated CpG-treated mouse model were generated as previously described.5 (link),24 (link),25 (link) All animal studies were performed according to an approved Institutional Animal Care and Use Committee protocol and federal regulations.
Huang R., Hayashi Y., Yan X., Bu J., Wang J., Zhang Y., Zhou Y., Tang Y., Wu L., Xu Z., Liu X., Wang Q., Zhou J., Xiao Z., Bridges J.P., Marsh R.A., Zhang K., Jordan M.B., Li Y, & Huang G. (2017). HIF1A is a critical downstream mediator for hemophagocytic lymphohistiocytosis. Haematologica, 102(11), 1956-1968.
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3

Genetic Manipulation of Autophagy Genes

Cited 4 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Atg5f/f and
Atg5f/f-Lyz2cre+/−mice were generated as described previously in an enhanced barrier
facility17 (link),56 (link).
Becn1f/f-
Lyz2cre+/−57 (link),
Fip200f/f-
Lyz2cre+/−58 (link),
Atg7f/f-Lyz2cre+/−51 (link), and
Atg16l1f/f-
Lyz2cre+/− 59 (link) were generated in the same way as
Atg5f/f-
Lyz2cre+/−.
Becn1f/f-
CD11c-cre+/−, and
Becn1f/f-
Mrp8-cre+/− were generated by breeding
Becn1f/f to CD11c-
cre+/− (#007567) and
Mrp8-cre+/− (#021614) from the Jackson
Laboratory. Rag1−/− (#002216),
Ifngr−/− (#003288), and
Casp1/11−/− (#016621) mice were
from the Jackson Laboratory.
Rubicon−/− knockout mice were
kindly provided by Doug Green and Jennifer Martinez25 (link). All mice used for experimental
procedures were backcrossed in house to B6/J except for
Rubicon−/− mice. 8–12 weeks
of age and sex-matched littermates were used unless specified otherwise and were
subject to randomization. Statistical consideration was not used to determine
mouse sample sizes. Mice were housed and bred at Washington University in St.
Louis in specific pathogen-free conditions in accordance with federal and
university guidelines, and protocols were approved by the Animal Studies
Committee of Washington University.
Wang Y.T., Zaitsev K., Lu Q., Li S., Schaiff W.T., Kim K.W., Droit L., Wilen C.B., Desai C., Balce D.R., Orchard R.C., Orvedahl A., Park S., Kreamalmeyer D., Handley S.A., Pfeifer J.D., Baldridge M.T., Artyomov M.N., Stallings C.L, & Virgin H.W. (2020). Select autophagy genes maintain quiescence of tissue-resident macrophages and increase susceptibility to Listeria monocytogenes. Nature microbiology, 5(2), 272-281.
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4

Tumor Immunotherapy in Mice

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C57BL/6 (B6), B2m–/–, Ifng–/–, Il2rb2–/–, Ifngr–/–, Il12r–/–, and Act-OVA mice were purchased from The Jackson Laboratory. Hemizygous OT-I mice on a CD45.1 background were generated in-house. Six- to 12-week-old age- and sex-matched mice were used. Mice of the same sex were randomly assigned to the experimental groups or were assigned on the basis of tumor burden, such that mice in all experimental groups had similar average tumor volumes prior to treatment.
Veatch J.R., Singhi N., Srivastava S., Szeto J.L., Jesernig B., Stull S.M., Fitzgibbon M., Sarvothama M., Yechan-Gunja S., James S.E, & Riddell S.R. (2021). A therapeutic cancer vaccine delivers antigens and adjuvants to lymphoid tissues using genetically modified T cells. The Journal of Clinical Investigation, 131(16), e144195.
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