HUVECs were grown in endothelial cell growth medium-2 (EGM-2; Lonza, Basel, Switzerland) supplemented with 1% penicillin-streptomycin (WelGENE, Daegu, Korea) at 37°C in a 5% CO2 incubator. HEK293T cells were cultured in HyClone Dulbecco’s modified Eagle’s medium (DMEM; GE Healthcare Life Sciences, Marlborough, IL, USA) containing 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA) and 1% penicillin-streptomycin. Both cell types were cultured to 70–80% confluency before each experiment.
Hyclone dulbecco s modified eagle s medium dmem
Manufactured by GE Healthcare
Sourced in United States
HyClone™ Dulbecco's modified Eagle's medium (DMEM) is a widely used cell culture medium formulation. It provides essential nutrients and components required for the in vitro cultivation of a variety of cell types.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
Egm 2, by Lonza (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Nerve growth factor (ngf), by Thermo Fisher Scientific (1 mentions)
Nsc 34, by American Type Culture Collection (1 mentions)
Hek293t, by Corning (1 mentions)
Gene pulser 2, by Bio-Rad (1 mentions)
Pc12 cell line, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
L glutamine, by Merck Group (1 mentions)
Pyruvate, by Merck Group (1 mentions)
Bovine α la, by Merck Group (1 mentions)
9 protocols using hyclone dulbecco s modified eagle s medium dmem
1
Culturing Human Cell Lines
2
Huntington's Disease Striatal Cell Model
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HdhQ7 and Q111 mouse striatal cells were derived from a knock-in transgenic mouse model with either 7-polyglutamine repeats (Q7, wild-type) or 111-polyglutamine repeats (Q111, HD) in the Huntingtin gene. These cells were maintained in HyClone™ Dulbecco’s modified Eagle’s medium (DMEM, GE Healthcare Life Sciences) supplemented with 10% fetal bovine serum (FBS, Corning), 1% penicillin/streptomycin, and 0.4 mg/mL G418. Cultures were maintained at 33 °C in a humidified atmosphere containing 5% CO2. The cells were maintained below 12 passages.
3
Culturing Mouse Motor Neurons and Cell Lines
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HyClone™ Dulbecco’s modified Eagle’s medium (DMEM) (GE Healthcare Life Science) with 10% fetal bovine serum (FBS) (Corning) was used for mouse motor neuron-like hybrid cell line (NSC-34), PC12 cells, HEK293T, and BMSCs culture. The cell lines of NSC-34, PC12, and HEK293T were bought from American Type Culture Collection (ATCC, USA). PC12 cells were incubated by culture medium containing 50 ng/ml NGF (Peprotech, 450-34) for 24 h and keep passage for the following experiment. BMSCs were isolated from the fetuses of SD rats as previously described [21 (link)]. All cells were maintained at 37 °C in a humidified incubator with 5% CO2 with cell passage performed, when cell density was ~ 90%. Cryopreservation solutions used comprised of complete medium and 10% dimethyl sulfoxide (DMSO).
4
Cytotoxicity of ZnO Nanoparticles
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LCs and SCs were cultured in HyClone™ Dulbecco’s Modified Eagles Medium (DMEM) (GE Healthcare Bio-Sciences, Marlborough, MA, USA) supplemented with fetal bovine serum (10%) and antibiotics (penicillin 100 U/mL and streptomycin 100 μg/mL) at 37°C in 5% CO2 atmosphere. Cells were seeded at a density of 1×104 cells per well and cultured for 24 hours before washing with phosphate-buffered saline (PBS; pH 7.4) and incubated in a fresh medium containing different concentrations of ZnO NPs as indicated in the “Results” section. ZnO NPs were sonicated before use in cell culture.
5
Cell Line Transfection by Electroporation
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The SiHa, CaSki, C33A and HEK293 cell lines, purchased from teh American Type Culture Collection (Manassas, VA, USA) were grown in HyClone Dulbecco's modified Eagle's medium (DMEM; GE Healthcare Life Sciences) supplemented with 10% fetal bovine serum (FBS), 50 U/ml penicillin, 50 µg/ml streptomycin and 2 mM L-glutamine. The cells were transfected by electroporation using a Bio-Rad Gene Pulser II electroporator (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
6
Huntington's Disease Striatal Cell Model
7
PC12 Cell Culture Maintenance
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HyClone™ Dulbecco's modified Eagle's medium (DMEM) (GE Healthcare Life Science) with 10% fetal bovine serum (FBS) (Corning) was used for PC12 cell culture. The PC12 cell line was purchased from American Type Culture Collection (ATCC, USA). Cells were maintained at 37°C in a humidified incubator with 5% CO2, and cell passage was performed when the cell density was ∼90%.
8
PC12 Cell Culture Protocol
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HyClone™ Dulbecco's modified Eagle's medium (DMEM) (GE Healthcare Life Science) with 10 % fetal bovine serum (FBS) (Corning) was used for PC12 cells culture. The cell lines of PC12 were bought from American Type Culture Collection (ATCC, USA). Cells were maintained at 37 °C in a humidified incubator with 5% CO2 with cell passage performed, when cell density was ~ 90%.
9
Bovine α-LA Lipid Metabolism Assay
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Bovine α-LA (≥85% purity), oleic acid (OA, C18: 1: 9 cis, ≥99.0% purity, cell culture tested), glucose, l-glutamine, and pyruvate were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO). HyClone Dulbecco's modified Eagle's medium (DMEM) containing a high level of glucose was purchased from GE Healthcare (Chicago, IL). Fetal bovine serum was purchased from Gibco (Thermo Fisher Scientific, Carlsbad, CA). All other chemicals used were of analytical grade.
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