Eugenol ReagentPlus® 99% and acetonitrile Chromasolv® ≥ 99.9% were purchased from Sigma-Aldrich (St. Louis, MO, USA). The clove essential oil was purchased from Mundo dos Óleos (Brasília, DF, Brazil). According to the manufacturer, the clove essential oil was obtained by steam distillation of Eugenia caryophyllata leaves.
Acetonitrile chromasolv
Manufactured by Merck Group
Sourced in United States
Acetonitrile Chromasolv is a high-purity solvent used in liquid chromatography and other analytical applications. It is a colorless, volatile liquid with a characteristic odor. Acetonitrile Chromasolv is designed to meet the requirements of sensitive analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Methanol chromasolv, by Merck Group (2 mentions)
Milli q reference a system, by Merck Group (2 mentions)
Rifampin, by Merck Group (1 mentions)
Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions)
Hepatocyte culture medium, by Corning (1 mentions)
Gentamycin sulfate, by Thermo Fisher Scientific (1 mentions)
Testosterone, by Merck Group (1 mentions)
Sybr greener qpcr supermix, by Thermo Fisher Scientific (1 mentions)
Hek293 mock cells, by Corning (1 mentions)
Its culture supplement, by Corning (1 mentions)
Epidermal growth factor (egf), by Corning (1 mentions)
Hek293 cells, by Corning (1 mentions)
Superscript first strand synthesis kit, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Diclofenac, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Corning (1 mentions)
5 protocols using acetonitrile chromasolv
1
Extraction and Analysis of Clove Oil
2
In Vitro Transporter Assay Protocol
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Rifampin, diphenhydramide hydrochloride (98%), dimethyl sulfoxide (DMSO), ammonium acetate, acetonitrile Chromasolv (ACN), formic acid, dexamethasone, diclofenac, thiazolyl blue tetrazolium bromide (MTT), and methanol Chromasolv were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bosentan was purchased from Actelion Pharmaceuticals (Allschwil, Switzerland). Cryopreserved human hepatocytes, HEK293 cells overexpressing OATP1B1, OATP1B3, and OATP2B1 transporter, HEK293 mock cells, phosphate-buffered saline (PBS), Gentest High Viability CryoHepatocyte Recovery kit, hepatocyte culture medium, ITS+ culture supplement, Dulbecco’s Modification Eagle’s Medium (DMEM), Minimal Essential Medium (MEM) non-essential amino acid solution, epidermal growth factor (EGF), and Hank’s balanced salt solution (HBSS) were purchased from Corning (Corning, NY, USA). Penicillin/streptomycin, William’s E medium (without phenol red), fetal bovine serum (FBS), l -glutamine, gentamycin sulfate, SYBR Green ER qPCR Supermix, and Superscript First-strand Synthesis kit were purchased from Life Technologies (Burlington, ON, Canada). Sodium butyrate solution and testosterone were purchased from EMD-Millipore (Darmstadt, Germany) and Acros (Geel, Belgium), respectively. Easy Spin Total RNA extraction kit was purchased from iNtRON (Seongnam, Korea).
3
Colorimetric Assay Reagents for Analysis
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Reagents for colorimetric assays (Folin–Ciocalteu, DPPH, pyrocatechol violet and cinchonine hemisulfate, Trolox®, EDTA, sodium carbonate, sodium acetate, and copper sulfate) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile CHROMASOLV® (gradient grade, for HPLC, ≥99.9%) for LC-MS analysis was purchased from Sigma-Aldrich, and Milli-Q H2O was obtained in the laboratory using a Milli-Q Reference A+System (Merck Millipore, Burlington, MA, USA). Standards for LC-MS (gallic acid, catechin, epicatechin, ellagic acid, and (epi) gallocatechin) were purchased from Sigma-Aldrich.
4
Antioxidant Capacity Evaluation of Plant Extracts
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All enzymes, bile extract, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,4,6,-tris(2-pyridyl)-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), ferric chloride, aluminium chloride, Folin-Ciocalteu reagent (2N), vanillin, gallic acid, quercetin, catechin, the analytical standards for UHPLC analysis (vanillic acid, ferulic acid, cinnamic acid, and naringenin, all with purity ≥ 97%), methanol Chromasolv®, and acetonitrile Chromasolv® were purchased from Sigma-Aldrich (St. Louis, MO, USA). Luteolin and apigenin standards were obtained from Thermo Fischer Scientific (Heysham, Lancashire, UK). Other inorganic salts, hydrochloric acid, and sodium hydroxide were analytical grade (Lach-Ner, Neratovice, Czech Republic).
5
Curcumin Extraction and Analysis
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Ethyl acetate (ACS grade, ≥99%) (Sigma-Aldrich, Milan, Italy) was used in the extraction procedures. Acetonitrile CHROMASOLV® (gradient grade, for HPLC, ≥99.9%) for HPLC analysis was purchased from Sigma-Aldrich, while Milli-Q H2O was obtained in the laboratory using a Milli-Q Reference A+System (Merck Millipore). Standards of Curcumin (87.02% Curcumin, 12.98% other Curcuminoids) were purchased from Sigma Aldrich.
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