Ovaries were collected from mice upon euthanization and immediately placed in tissue fixation solution (60% ethanol, 30% formaldehyde, 10% glacial acetic acid) for 24 hours. The solution was changed to 70% ethanol the following day and replaced with fresh 70% ethanol after 24 hours. Tissue was dehydrated and processed for paraffin embedding. The ovaries were sectioned using a microtome at 20 μm, with three to four sections per slide, and dried overnight at 37°C. A hematoxylin and eosin stain (Sigma-Aldrich) was used to distinguish ovarian morphology. Corpora lutea and Graafian follicles were counted on every fifth section for each sectioned ovary. The section with the greatest number of corpora lutea or Graafian follicles was used as the representative number for that animal.
Hematoxylin and eosin stain
Manufactured by Merck Group
Sourced in United States
Hematoxylin and eosin stain is a common laboratory staining technique used to visualize the cellular and tissue structures in histological samples. Hematoxylin stains the nuclei of cells blue or purple, while eosin stains the cytoplasm and other structures pink or red. This staining method provides a contrast that allows for the identification of different cell types and tissue structures under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Peroxidase blocker, by Agilent Technologies (2 mentions)
Ls b48, by LSBio (2 mentions)
Target retrieval solution, by Agilent Technologies (2 mentions)
Vectastain elite abc kit, by Vector Laboratories (2 mentions)
Alfamec, by Alfasan (1 mentions)
Hyperforin, by Merck Group (1 mentions)
Formaline, by Merck Group (1 mentions)
Hyperoside, by Merck Group (1 mentions)
Hypericin, by Merck Group (1 mentions)
Quercetin dihydrate, by Carl Roth (1 mentions)
Masson s trichrome stain, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Pluronic f 127, by Merck Group (1 mentions)
Xylene, by Merck Group (1 mentions)
Crystal violet stain, by Merck Group (1 mentions)
Nutrient broth medium, by HiMedia (1 mentions)
Chloroauric acid haucl4 3h2o, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
9 protocols using hematoxylin and eosin stain
1
Ovarian Morphology Quantification
2
Alfamec 1% Injection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Alfamec® 1% was purchased from Alfasan Co., Woerden, The Netherlands; and the formula used for cattle and sheep injection was 1 ml Alfamec contains 10 mg IVM. The hematoxylin and eosin stain was procured from Sigma Chemical Co., Saint Louis, MO. Ethanol was purchased from Loba Chemie Co.; MDA, SOD, GSH, and GSH-Px Kits were procured from Biodiagnostic (Diagnostic and Research Reagents) Company, Dokki, Giza, Egypt. All other chemicals were of analytical grade and locally purchased.
3
Phytochemical Profiling and Formulation Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetonitrile, methanol, ortho-phosphoric acid (HPLC grade), rutin, hyperoside, hypericin and hyperforin were purchased from Sigma-Aldrich, Germany for phytochemical profiling. Quercetin dihydrate (≥98%) was obtained from Carl Roth (Karlsruhe, Germany). Hydroxyethyl cellulose (HEC), hydroxy propylmethyl cellulose (HPMC), sodium carboxymethyl cellulose (NaCMC), and Pluronic F-127 were purchased from Sigma-Aldrich, Germany for the formulations. Ketamine HCl 5% (Rotex medica, Trittau, Germany), and Panthenol® 2% cream (Nile Co. for Pharmaceuticals and Chemical Industries, Egypt) were obtained for the in vivo experiments. Formaline, xylene, hematoxylin and eosin stain, and Masson’s trichrome stain were sourced from Sigma-Aldrich, Germany for the histopathology. All other chemicals and reagents were of analytical grade.
4
Evaluation of Vancomycin-loaded Rice Granules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To find out whether
the vancomycin-loaded rice granules could be used as drug reservoirs
in the tissue, we tested an inflammatory reaction in mice. The animal
care and use protocol following the National Institutes of Health
(NIH; #85-23, revised 1985) was approved by the Institutional Animal
Care and Use Committee of the Faculty of Medicine, Chulalongkorn University,
Bangkok, Thailand. Male, C57BL/6 mice, 8-week-old, were purchased
from the National Laboratory Animal Center, Nakhornpathom, Thailand.
The subcutaneous injection of rice granules was performed under isoflurane
anesthesia under the sterile condition. In short, the hair above the
loose skin between shoulders was removed and cleaned with 70% alcohol.
Then, 0.375 mg of rice granules at the concentration of 2.5 mg/mL
dispersed in PBS or PBS control (150 μL) (n = 5/group, group I: injected with PBS only; group II: injected with
rice granules in PBS; group III: injected with vancomycin-loaded at
80% loading% rice granules in PBS) was administered subcutaneously.
The inflammatory skin reaction was observed daily. Mice were sacrificed
at 7 days post injection, and the skin lesion was fixed in 10% formalin,
paraffin-embedded, and stained with periodic acid-Schiff reagent Hematoxylin
and Eosin stain (Sigma-Aldrich, St. Louis, MO). The inflammatory cells
were observed from the lesion.
the vancomycin-loaded rice granules could be used as drug reservoirs
in the tissue, we tested an inflammatory reaction in mice. The animal
care and use protocol following the National Institutes of Health
(NIH; #85-23, revised 1985) was approved by the Institutional Animal
Care and Use Committee of the Faculty of Medicine, Chulalongkorn University,
Bangkok, Thailand. Male, C57BL/6 mice, 8-week-old, were purchased
from the National Laboratory Animal Center, Nakhornpathom, Thailand.
The subcutaneous injection of rice granules was performed under isoflurane
anesthesia under the sterile condition. In short, the hair above the
loose skin between shoulders was removed and cleaned with 70% alcohol.
Then, 0.375 mg of rice granules at the concentration of 2.5 mg/mL
dispersed in PBS or PBS control (150 μL) (n = 5/group, group I: injected with PBS only; group II: injected with
rice granules in PBS; group III: injected with vancomycin-loaded at
80% loading% rice granules in PBS) was administered subcutaneously.
The inflammatory skin reaction was observed daily. Mice were sacrificed
at 7 days post injection, and the skin lesion was fixed in 10% formalin,
paraffin-embedded, and stained with periodic acid-Schiff reagent Hematoxylin
and Eosin stain (Sigma-Aldrich, St. Louis, MO). The inflammatory cells
were observed from the lesion.
5
Tumor Tissue Fixation and Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After measuring the tumor volume, the tumor tissues were fixed with 10% formaldehyde for 24 h and embedded in paraffin. Sections (2 μm) were stained with hematoxylin and eosin. The hematoxylin and eosin stain was purchased from Sigma-Aldrich (St. Louis, MO, USA).
6
Immunohistochemical Analysis of Lung Tissue
7
Synthesizing Gold Nanoparticles and Evaluating Cytotoxicity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chloroauric acid (HAuCl4.3H2O) was purchased from Sigma, USA. Zinc granulated metal (Zn) at a purity of 99.9% was purchased from VWR International, USA. Muller–Hinton agar and nutrient broth medium were purchased from HiMedia (India). The rat embryonic fibroblast (REF) cell line was kindly gifted from the Iraqi Center for Cancer and Medical Genetics Research (ICCMGR). RPMI-1640 medium was purchased from Gibco (USA). Fetal calf serum, streptomycin, penicillin, acridine orange, ethidium bromide, trypsin-EDTA, and crystal violet stain were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Αlpha-hemolysin and b-tubulin antibodies were purchased from Abcam. Hematoxylin and eosin stains were purchased from Sigma (USA). All other chemicals and reagents were of the analytical grade level.
8
Immunohistochemical Analysis of Lung Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We prepared and stained formalin-fixed, paraffin-embedded 5-micron-thick lung sections using standard procedures. Briefly, sections were deparaffinized through graded alcohols and washed in PBS. Heat-activated antigen retrieval was performed in a microwave oven using a citrate buffer (Target Retrieval Solution, S1699, DAKO) for 10 minutes. Endogenous peroxidases were blocked using a peroxidase blocker (DAKO Cytomation), and the slides were blocked for nonspecific protein binding by incubating in 10% normal serum for 45 minutes. Tissues were immunostained with a rabbit primary IgG to Irp2 (LS-B48, LS Bio) at a 1:50 dilution or with mouse anti-cytochrome C, (556433, BD Biosciences) at a 1:250 dilution or with rabbit monoclonal IgG to cytochrome C EPR1327 (D00355 Abcam) at a 1:50 dilution, or with rabbit anti-LC3B (L7543, Sigma Aldrich) at a 1:400 dilution and signals were developed using the VECTASTAIN Elite ABC Kit (PK-6101; Vector Laboratories), according to the manufacturers protocol. Hematoxylin and Eosin stains were purchased from Sigma Aldrich and staining was carried out as per manufacturer’s instructions. The negative control consisted of substituting PBS for the primary antibody.
9
Hematoxylin and Eosin Staining Protocol
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!