Mgc anaeropack jar

Four active components of pomegranate, punicalagin (1), punicalin (2), strictinin A (3), and granatin B (4), were isolated and identified in our previous study (Figure 3) [13 (link)]. 3-(4.5-Dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), lipopolysaccharide (LPS), and other chemicals were purchased from Sigma Industries (Saint Louis, MO, USA). Dulbecco’s modified Eagle medium (DMEM), Roswell Park Memorial Institute (RPMI) medium, fetal bovine serum (FBS), anti-biotics, and glutamine were purchased from GIBCO BRL (Grand Island, NY, USA). The bacterial culture equipment, including an anaerobic atmosphere by MGC AnaeroPack-Anaero and MGC AnaeroPack-Jar, were purchased from Mitsubishi Gas Chemical Company (Tokyo, Japan). Blood agar plates (BAPs), Bacto™ tryptic soy broth (TSB), and Bacto™ tryptic soy agar (TSA) were purchased from Difco (Detroit, MI, USA). The assay kits for tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-8 were purchased from eBioscience (San Diego, CA, USA).

Six components of S. flexibilis including, 11-dehydrosinulariolide (SC-2), sinulariolide (SC-4), dendronpholide F (SC-6), 3,4:8,11-bisepoxy-7-acetoxycembra-15(17)-en-1,12-olide (SC-7), sinularin (SC-9), and dihydrosinularin (SC-10), were isolated and identified by Prof. Jui-Hsin Su (National Museum of Marine Biology and Aquarium, Pintung, Taiwan) [23 (link)]. Dimethyl sulfoxide (DMSO), LPS, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), napthyl-ethylene-dihydrochloride, sulfanilamide, and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). RAW 264.7 (BCRC 60001) and HaCaT cells were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), antibiotics, and glutamine were purchased from GIBCO BRL (Grand Island, NY, USA). The bacterial culture equipment, including an anaerobic atmosphere by MGC AnaeroPack-Jar and MGC AnaeroPack-Anaero, were purchased from Mitsubishi Gas Chemical (Tokyo, Japan). Blood agar plates (BAPs), BactoTM tryptic soy agar (TSA), and BactoTM tryptic soy broth (TSB) were purchased from Difco (Detroit, MI, USA).

Myristicin (≥98.5%, Merck code 09237), safrole (≥98.5%, Supelco code 08010), dimethyl sulfoxide (DMSO), lipopolysaccharide (LPS), 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT), and other chemicals were purchased from Sigma (St. Louis, MO, USA). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), antibiotics, and glutamine were purchased from GIBCO BRL (Grand Island, NY, USA). The bacterial culture equipment, including an anaerobic atmosphere by MGC AnaeroPack-Jar and MGC AnaeroPack-Anaero, were purchased from Mitsubishi Gas Chemical (Tokyo, Japan). Blood agar plates (BAPs), Bacto^TM tryptic soy agar (TSA), and BactoTM tryptic soy broth (TSB) were purchased from Difco (Detroit, MI, USA).