Rabbit anti c rel

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit anti-c-Rel is a primary antibody directed against the c-Rel protein. c-Rel is a transcription factor that plays a role in regulating immune and inflammatory responses. This antibody can be used in various immunological applications to detect and study the c-Rel protein.

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C-Rel (30 citations) Anti-c-Rel (8 citations) Rabbit anti-TM (2 citations)

2 protocols using rabbit anti c rel

Western Blot Analysis of Protein Biomarkers

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Proteins from tissues or cells were separated by SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membrane. Membrane was subsequently incubated with primary antibodies: Rabbit anti-Bcl-2 (1:500; Cell Signal Technology, USA), Rabbit anti-Bcl-xl (1:500; Abways, China), mouse anti-TH (1:2000; Sigma, USA), Rabbit anti-GFAP (1:2000; Dako, Japan), Rabbit anti-iNOS (1:500; Abcam, USA), Rabbit anti-COX2 (1:1000; Abcam, USA), Rabbit anti-IL-1β (1:1000; Santa Cruz, USA), Rabbit anti-Bax (1:1000; Cell Signal Technology, USA), Rabbit anti-SOD2 (1:1000; Abcam, USA), Rabbit anti-c-Rel (1:250; Santa Cruz, USA), Rabbit anti-H3 (1:1000; Cell Signal Technology, USA) and mouse anti-β-actin (1:2000; Santa Cruz, USA). Protein bands were detected and imaged using an Odyssey infrared imaging system (Li-Cor, USA). Densities were quantified using Quantity One 4.5.2 software (Bio-Rad, Hercules, USA).

Wang Z., Dong H., Wang J., Huang Y., Zhang X., Tang Y., Li Q., Liu Z., Ma Y., Tong J., Huang L., Fei J., Yu M., Wang J, & Huang F. (2020). Pro-survival and anti-inflammatory roles of NF-κB c-Rel in the Parkinson's disease models. Redox Biology, 30, 101427.

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Western Blot Analysis of Neuronal Proteins

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Western blot analysis was performed as described to analyze the expression of p65, c-Rel, Bcl-xL, Bax and β-Actin in neurons from each group [46 (link)]. Cell extracts were collected on ice in a RIPA lysis buffer (Beyotime, China) and then centrifuged at 14000×g at 4°C for 10 min. Proteins were separated on 12% SDS-polyacrylamide gels and then electrotransferred onto a PVDF membrane (Millipore, USA). The membranes were blocked in 5% non-fat milk in TBS and probed with primary antibodies to rabbit anti-p65 (1:500, Santa Cruz, USA), rabbit anti-c-Rel (1:500, Santa Cruz, USA), rabbit anti-Bcl-xL (1:1000, Abcam, UK), rabbit anti-Bax (1:1000, Abcam, UK), rabbit anti-β-Actin (1:1000, Abcam, UK) and then incubated with a goat anti-rabbit IgG-HRP secondary antibody (1:8000, Abcam, UK). The optical densities of the antibody-specific bands were detected with ECL reagent kits and analyzed with Image J software (1.46r).

Chen H., Lin W., Lin P., Zheng M., Lai Y., Chen M., Zhang Y., Chen J., Lin X., Lin L., Lan Q., Yuan Q., Chen R., Jiang X., Xiao Y, & Liu N. (2019). IL-10 produces a dual effect on OGD-induced neuronal apoptosis of cultured cortical neurons via the NF-κB pathway. Aging (Albany NY), 11(23), 10796-10813.

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