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2 protocols using α naphthylamine

1

Plant Cell Culture Optimization

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Murashige and Skoog medium, 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, 2-(N-morpholino)ethanesulfonic acid (MES), triphenil-tetrazolium chloride (TTC), xylenol orange, EDTA, succinate, 4-morpholinepropanesulfonic acid (MOPS), Polyvinylpyrrolidone (PVP-40), hydroxylamine, sulphanilamide, α-naphthylamine, ampicillin, NP40, safranin, Salicylhydroxamic acid (SHAM), kalium-cyanide (KCN), linoleic acid, fatty acid free bovine serum albumin (BSA), luminol, p-Coumaric acid, Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), were obtained from Sigma-Aldrich. ProBond Purification System was purchased from Invitrogen. Amicon Ultra 30K Centrifugal Filter Units were purchased from Merck. IPTG was obtained from Duchefa Biochemie, cytochrome c was purchased from Fluka. Primary and secondary antibodies were purchased from Agrisera Antibodies. All other chemicals were of analytical or HPLC grade, and were purchased from Reanal, Hungary. Pierce BCA Protein Assay Kit, GeneJET Plant RNA Purification Kit, and RevertAid First-Strand cDNA Synthesis Kit were obtained from Thermo Scientific; SensiFAST SYBR No-ROX Kit was purchased from Bioline.
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2

Microcystin-LR Quantification Protocol

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Microcystin-LR, ≥95% in purity (HPLC) was purchased from Taiwan Algae Institute Ltd. (Taoyuan City, Taiwan). The Microcystin Plate Kit was purchased from the Institute of Hydrobiology, Chinese Academy of Sciences. Phenylmethylsulfonyl fluoride (PMSF), o-phthaldialdehyde, bovine serum albumin (BSA), polyvinylpolypyrrolidone (PVPP), and α-naphthylamine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, acetic acid, and dimethyl sulfoxide (DMSF) were purchased from Merck (Darmstadt, Germany). Other reagents were analytical grade and purchased from Chinese companies.
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