Pcdna4 vps34 flag
The pcDNA4-VPS34-FLAG is a plasmid vector that expresses the VPS34 protein with a FLAG tag. VPS34 is a phosphoinositide 3-kinase that plays a role in the regulation of various cellular processes, including autophagy and endocytosis. The FLAG tag allows for the detection and purification of the expressed VPS34 protein.
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6 protocols using pcdna4 vps34 flag
Molecular Cloning of Autophagy Proteins
Ubiquitin Mutant Transfection in HEK293 and HT22 Cells
pcDNA4-Vps34-Flag was purchased from Addgene (#24398). All ubiquitin mutants were made from pRK5-HA-ubiquitin plasmid (Addgene) by mutagenesis. pRK5-HA-Ubiquitin-K0 (no lysine), pRK5-HA-Ubiquitin-K6 (keep lysine at position 6), pRK5-HA-Ubiquitin-K11, pRK5-HA-Ubiquitin-K27, pRK5-HA-Ubiquitin-K29, pRK5-HA-Ubiquitin-K33, pRK5-HA-Ubiquitin-K48, pRK5-HA-Ubiquitin-K63. 24 h before transfection, cells were seeded to obtain a final confluence of 60–70%. Transfections were carried out using Lipofectamine™ 2000 (Thermo Fisher Scientific, #11668019) according to manufacturer's protocols. PcDNA3.1 was used as an expression control. siRNA sequence for UBE2N was: GAACCAGTTCCTGGCATCA (RiboBio silencer, ID#stB000527A).15 (link)
Vps34 Regulation by Umbelliprenin
BCRP3 Regulation via shRNA
Autophagy Regulation by TcdB Glucosyltransferase
Rab5-Mediated Vesicle Dynamics Protocol
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