Hitrap mabselect sure 5 ml column

Manufactured by GE Healthcare

The HiTrap mAbSelect SuRe 5 mL column is a pre-packed column designed for the purification of monoclonal antibodies (mAbs) and antibody fragments. The column utilizes a recombinant protein A-based ligand that is covalently coupled to a highly cross-linked agarose matrix, providing a robust and reliable purification platform.

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Lab products found in correlation

Hitrap, by Cytiva (2 mentions) Mabselect sure, by Cytiva (2 mentions) Vivaspin turbo 15, by Sartorius (1 mentions) Streptavidin magnesphere paramagnetic particles, by Promega (1 mentions) Alexa fluor 488 conjugated goat anti human igg, by Jackson ImmunoResearch (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Hoechst 33342 trihydrochloride, by Thermo Fisher Scientific (1 mentions) Nta chip, by GE Healthcare (1 mentions) Sypro orange, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti flag m2 antibody, by Merck Group (1 mentions) Hrp conjugated goat anti mouse igg, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

MabSelect SuRe (96 citations) HiTrap MabSelect SuRe (38 citations) MabSelect SuRe column (17 citations) HiTrap columns (11 citations) HiTrap MabSelect column (5 citations)

2 protocols using hitrap mabselect sure 5 ml column

Iratumumab Expression and Purification

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Example 2

Iratumumab was transiently expressed in CHO K1 cells by Evitria (Zurich, Switzerland) at 125 mL scale. The supernatant was purified using a HiTrap mAbSelect SuRe 5 mL column (GE Healthcare). The supernatant was loaded onto the column followed by washing with at least 10 column volumes of 25 mM Tris pH 7.5, 150 mM NaCl. Retained protein was eluted with 0.1 M Glycine pH 2.7. The eluted product was immediately neutralized with 1.5 M Tris-HCl pH 8.8 and dialyzed against 20 mM Tris pH 7.5. Next the product was concentrated to 14.4 mg/mL using a Vivaspin Turbo 15 ultrafiltration unit (Sartorius) and stored at −80° C. prior to further use.

US11590239B2. Antibody-conjugates with improved therapeutic index for targeting CD30 tumours and method for improving therapeutic index of antibody-conjugates (2023-02-28). Synaffix B.V. [NL]. Inventors: Sander Sebastiaan Van Berkel [NL], Jorge Merijn Mathieu Verkade [NL], Maria Antonia Wijdeven [NL], Ryan Heesbeen [NL], Petrus Josephus Jacobus Maria Van De Sande [NL], Remon Gvan Eel [NL], Brian Maria Gerardus Janssen [NL], Inge Catharina Josephina Hurkmans [NL], Floris Louis Van Delft [NL].

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Comprehensive Molecular Biology Protocol

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All the enzymes used in molecular biology works are from Fermentas and NEB. cDNA clone of human calmodulin (Cat no: SC115829) is from OriGene. Streptavidin MagneSphere Paramagnetic Particles (SA coated magnetic beads) are from Promega and NHS activated magnetic beads are purchased from Pierce. HRP conjugated anti-M13 mouse monoclonal antibody (Cat no: 27942101) is from GE Healthcare Life Sciences. Anti-FLAG M2 mouse monoclonal antibody (Cat no: F3165) and its HRP conjugate (Cat no: A8592) are from Sigma-Aldrich. HRP conjugated goat anti-mouse IgG (Cat no: 115-035-003) and AlexaFluor 488 conjugated goat anti-human IgG (Cat no: 109-546-097) are from Jackson ImmunoResearch. Hoechst 33342 trihydrochloride (Cat no: H3570) and ProLong Gold antifade reagent are from Invitrogen. Sypro Orange and SuperSignal West Pico chemiluminscent substrate are purchased from Life Technologies. NTA chip, Resource S 1ml column and HiTrap MabSelect SuRe 5 ml column are from GE Healthcare Life Sciences. E. coli DH5α and XL1-Blue cells are from Agilent Life Sciences and BL21, BL21(DE3), Tuner(DE3) are from Novagen. All the other reagents and chemicals used are of analytical grade.

Mukherjee S., Ura M., Hoey R.J, & Kossiakoff A.A. (2015). A new versatile immobilization tag based on the ultra high affinity and reversibility of the calmodulin-calmodulin binding peptide interaction. Journal of molecular biology, 427(16), 2707-2725.

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