Milli q biocel a10 purification unit

Bacto-tryptone and yeast extract were obtained from Biokar Diagnostics (FR). LB medium was purchased from Sigma-Aldrich (FR). Ampicillin was purchased from Eurobio (FR). Glycerol and isopropyl β-D-thiogalactopyranoside (IPTG) were purchased from Euromedex (FR).
Glycidyl propargyl ether was obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, FR).
Glacial acetic acid, Trizma® and Hexafluoroisopropanol (HFIP) were obtained from Sigma-Aldrich (FR). Deionized water (18 MΩ-cm) was obtained by using a Millipore Milli-Q Biocel A10 purification unit. Cuprisorb was purchased from Seachem. Ethanol (96.0%, EtOH), mEthanol (98.5%, MeOH) and acetonitrile (99.9%, ACN) were obtained from VWR international. NaCl (99%) was purchased from Alfa Aesar (FR). Azide monosaccharides (β-Dgalactopyranosyl azide, Gal-N 3 ; and β-D-glucopyranosyl azide (Glu-N 3 ) were obtained from Carbosynth (UK). Ammonium Acetate and Ammonium pyrrolidinedithiocarbamate, APDC, were purchased from Fisher Scientific (FR). RCA 120 and RCA 120 -Fluorescein were purchased from Eurobio (FR). Human serum from human male AB plasma, USA origin, sterile-filtered, was used as received from Sigma-Aldrich (FR). N,N,N′,N′′,N′′-pentamethyldiethylenetriamine (PMDETA) was purchased from Sigma-Aldrich (FR). CuSO 4 •5H 2 O was obtained from VWR (FR). Sodium ascorbate was obtained from Fisher Scientific (FR).