Gallic acid

Manufactured by Sinopharm

Sourced in China

Gallic acid is a phenolic compound found in various plant sources. It is a crystalline solid with a molecular formula of C6H2(OH)3COOH. Gallic acid is commonly used as a reagent in analytical chemistry and as a precursor in the synthesis of other organic compounds.

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Gallic (2 citations)

25 protocols using gallic acid

Phytochemical Extraction and Quantification

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Ascorbic acid, gallic acid,
vanilline, and Folin-Ciocalteu reagent were purchased from Sinopharm
Chemical Reagent Co., Ltd (Shanghai, China). Rutin and catechin were
bought from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China).
Ursolic acid and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic
acid) (ABTS) were, respectively, purchased from Cool Chemistry Co.,
Ltd (Beijing, China) and Shanghai Aladdin Bio-Chem Technology Co.,
Ltd (Shanghai, China). Hoagland reagents were bought from Qingdao
Hope Bio-Technology Co., Ltd (Qingdao, China). All other chemicals
or reagents were of analytical or HPLC grade.

Jiang B., Geng F., Chang R., Ruan M., Bian Y., Xu L., Feng F, & Chen Q. (2020). Comprehensive Evaluation of the Effect of Ultraviolet Stress on Functional Phytochemicals of Hulless Barley (Qingke) Grass in Different Growth Times at Vegetative Stage. ACS Omega, 5(49), 31810-31820.

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Phytochemical Analysis and Antioxidant Assay

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All chemicals and reagents used were of analytical grade. The water was double distilled (ddH₂O). Methanol and acetonitrile of chromatographic purity were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-hexane, ethyl acetate, gallic acid, NaOH, Folin–Ciocalteu, Na₂CO₃, Trolox, 2,2-diphenyl-1-picrylhydrazyl (DPPH), FeCl₃, HCl, NaAc, and 2,4,6-tris (2-pyridyl)-s-triazine (TPTZ) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Gao H., Guo M., Wang L., Sun C, & Huang L. (2022). Identification and Antioxidant Capacity of Free and Bound Phenolics in Six Varieties of Mulberry Seeds Using UPLC-ESI-QTOF-MS/MS. Antioxidants, 11(9), 1764.

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Mung Bean Hull Dietary Fiber Extraction

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The soluble dietary fiber (SDF) of mung bean (Vigna radiata L.) hull was bought from Watson Biotech Co., Ltd. (Hangzhou, China). Canned tuna FF was supplied by Zhejiang Ocean Family Co., Ltd. (Hangzhou, China). The tea polyphenol (TPP), sodium alginate, chitosan, acetic acid, Span 80, Tween 80, gelatin glycerin, and calcium chloride were food‐grade and purchased from Boyan Food Co., Ltd. (Yangzhou, China). Gallic acid, sodium carbonate, magnesium chloride hexahydrate, and potassium chloride were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Zhang M., Zhao Q., Lin Y., Wang H., Shui R., Wang S., Ge L., Li Y., Song G., Gong J., Wang H., Chen X, & Shen Q. (2022). Fabrication and characterization of tea polyphenol W/O microemulsion‐based bioactive edible film for sustained release in fish floss preservation. Food Science & Nutrition, 10(7), 2370-2380.

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Arabica Coffee Compound Analysis

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Arabica coffee (originating from Brazil) from Basic ElementsCatering Services Co., LTD (Gu'an, China) were was used in all the trials. Caffeine, CGA, and trigonelline were obtained from Chengdu Effa Biological Technology Co., Ltd (Chengdu, China). Folin–Ciocalteu was purchased from Coolaber Technology Co., Ltd (Beijing, China). Other chemicals, such as gallic acid, sodium carbonate, n-hexane, and sodium chloride, were of analytical grade and secured from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).

Zhai X., Yang M., Zhang J., Zhang L., Tian Y., Li C., Bao L., Ma C, & Abd El-Aty A.M. (2022). Feasibility of Ultrasound-Assisted Extraction for Accelerated Cold Brew Coffee Processing: Characterization and Comparison With Conventional Brewing Methods. Frontiers in Nutrition, 9, 849811.

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Extraction and Characterization of Onion Polyphenols

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Total polyphenols from onion, according to gallic acid content calibration (polyphenols content of 68%) were isolated in-house. 2-Diphenyl-1-picrylhydrazine radical (DPPH), gallic acid, X, allopurinol (AL), and XO were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). All other chemicals and solvents used were HPLC grade from Hunan Chemical Reagent Factory (Changsha, China) and used as received or dried by standard procedures, unless stated otherwise.

Ouyang H., Hou K., Peng W., Liu Z, & Deng H. (2017). Antioxidant and xanthine oxidase inhibitory activities of total polyphenols from onion. Saudi Journal of Biological Sciences, 25(7), 1509-1513.

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Strawberry Phytochemical Extraction

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The strawberries (Fragaria × ananassa Duch. cv. HongYan) were purchased from a strawberry farm in Danyang, Jiangsu, China. Mature strawberries of uniform size and color without mechanical damage/fingerprints were selected as the experimental subjects and kept under cold storage (4 °C) until they were used. All reagents such as methanol, gallic acid, Folin–Ciocalteu, rutin, and vitamin C were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Feng M., Chitrakar B., Chen J., Islam M.N., Wei B., Wang B., Zhou C., Ma H, & Xu B. (2022). Effect of Multi-Mode Thermosonication on the Microbial Inhibition and Quality Retention of Strawberry Clear Juice during Storage at Varied Temperatures. Foods, 11(17), 2593.

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Shaoxing Huangjiu Production Protocols

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The 20 L mechanical craft semidry (total sugar ranged from 15 to 40 g/L) huangjiu used in this study was sourced from a local winery in Shaoxing, Zhejiang province, China. All analytically pure reagents used, including absolute ethanol, sodium hydroxide, hydrochloric acid, phenol, concentrated sulfuric acid, anhydrous glucose, Folin phenol, and gallic acid, were procured from Sinopharm Reagent Co., Ltd., Shanghai, China. For the sensory test, food-grade sodium chloride, citric acid, sucrose, quinine, glutathione, and monosodium glutamate (MSG) were purchased from Gino Biotech Co., Ltd., Quzhou, China. The purification equipment, specifically the membrane filtration module, was acquired from Shaoxing Haina Co., Ltd., Shaoxing, China.

Chang R., Zhou Z., Dong Y., Xu Y., Ji Z., Liu S, & Mao J. (2023). Sensory-Guided Isolation, Identification, and Active Site Calculation of Novel Umami Peptides from Ethanol Precipitation Fractions of Fermented Grain Wine (Huangjiu). Foods, 12(18), 3398.

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Enzymatic Assay of DPP-IV Activity and Inhibition

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The enzyme, human recombinant DPP-IV (Lot: D4943-1VL), expressed in baculovirus-infected S/9 cells, the substrate, Gly-Pro p-nitroanilide (Gly-Pro pNA; Lot: G0513), and streptozotocin (STZ; Lot: V900890) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The antidiabetic drug sitagliptin (Januvia 100 mg; Merk Sharp and Dohme Ltd., Hertfordshire EN11 9BU, Hertford Road, Hoddesdon, UK) was purchased at a drugstore in China. Other reagents, such as Folin–Ciocalteu (Lot: 73104861), gallic acid (Lot: XW01499171), and Coomassie Blue R-250 (Lot: 71011381) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Lapsang souchon (LS), a typical Chinese black tea, was obtained from a tea market in Hangzhou.

Lu Y., Lu P., Wang Y., Fang X., Wu J, & Wang X. (2019). A Novel Dipeptidyl Peptidase IV Inhibitory Tea Peptide Improves Pancreatic β-Cell Function and Reduces α-Cell Proliferation in Streptozotocin-Induced Diabetic Mice. International Journal of Molecular Sciences, 20(2), 322.

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Colored Potato Cultivar Characterization

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Four different cultivars of colored potatoes, including W2, W8, R69, and R16–5 are cultivated by Professor Qin Chen, Northwest A & F University. Gallic acid is purchased from Sinopharm Group (Shanghai, China). Rutin, Trolox, α-amylase (50 U/mg), cellulase (400 U/mg), pectinase (50,000 U/g) are purchased from Yuanye Biotechnology (Shanghai, China). Three different types of macroporous resins, D101, AB-8, and HPD100 are obtained from Xi'an Lanshen Special resin Co. (Shannxi, China), Anhui Samsung resin Co. (Anhui, China), and Xi'an Hanyu Resin Technology Co. (Shannxi, China), respectively.

Han L., Li R., Jin X., Li Y., Chen Q., He C, & Wang M. (2024). Metabolomic analysis, extraction, purification and stability of the anthocyanins from colored potatoes. Food Chemistry: X, 22, 101423.

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Purification and Antioxidant Evaluation of Borneol

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Crude borneol was provided by Fujian Green Pine CO., Ltd. and further purified (purity ≥97%) by Fujian Nanping Green Pine Chemical Co., Ltd. Copper chloride (CuCl₂), sodium sulfide, and gallic acid were offered by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dichlorofluorescein diacetate (DCFH-DA), thiazolyl blue tetrazolium bromide (MTT), and propidium iodide (PI) were provided by Beyotime Biotechnology Co., Ltd., Shanghai, China). Glutathione, polyvinylpyrrolidone (K30), and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) were available from Shenggong Biotech Co., Ltd. (China). Murine mammary carcinoma 4T1 cells and the cell culture medium were obtained from Keygen Biotech Co., Ltd. (China).

Huang B., Huang Y., Han H., Ge Q., Yang D., Hu Y., Ding M., Su Y., He Y., Shao J, & Chu J. (2021). An NIR-II Responsive Nanoplatform for Cancer Photothermal and Oxidative Stress Therapy. Frontiers in Bioengineering and Biotechnology, 9, 751757.

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