Hcc70

Manufactured by Thermo Fisher Scientific

Sourced in United States

The HCC70 is a benchtop centrifuge designed for general laboratory use. It features a fixed-angle rotor and can accommodate a variety of sample tube sizes. The HCC70 provides consistent and reliable centrifugation performance.

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Lab products found in correlation

7 protocols using hcc70

Maintenance of Breast Cancer Cell Lines

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Human breast cancer cell lines MCF7, MDA-MB-468, MDA-MB-231, HCC70, and SK-BR-3 and normal human mammary epithelial cell MCF10A were purchased from American Type Culture Collection (ATCC). We have authenticated all the cell lines by short tandem repeat technology (Genetica DNA Laboratories). Cells were tested for mycoplasma using the Universal Mycoplasma Detection Kit from ATCC. Cells were maintained at 5% CO₂ in a 37 °C humidified incubator. MCF7, MDA-MB-468, MDA-MB-231, and HCC70 cells were grown in DMEM (Invitrogen) and supplemented with 10% FBS (Gemini). SK-BR-3 cells were cultured in McCoy’s 5a Medium and supplemented with 10% FBS (Gemini). MCF10A cells were grown in MEGM Mammary Epithelial Cell Growth Medium SingleQuots Kit without gentamycin–amphotericin B mix (Lonza) with 100 ng/mL cholera toxin. All cells were supplemented with 50 U/mL penicillin, 50 μg/mL streptomycin (Mediatech), and 5 μg/mL plasmocin (InvivoGen). MDA-MB-468 shp53 cells generated with mir30 short hairpin RNA can induce knockdown of mtp53 with 8 μg/mL doxycycline for 7 days.^{3 (link),16 (link)}

Xiao G., Annor G.K., Fung K., Keinänen O., Zeglis B.M, & Bargonetti J. (2020). Targeting Triple Negative Breast Cancer with a Nucleus-Directed p53 Tetramerization Domain Peptide. Molecular pharmaceutics, 18(1), 338-346.

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Breast Cancer Cell Line Characterization

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All cell lines were purchased from the American Type Culture Collection (ATCC). The following Her2− human breast cancer cell lines were used: HCC38, HCC1395, HCC70, MDA231, MDA468, HCC1806, HCC1143 and HCC1937. The following HER2+ breast cancer cell lines were used: HCC202, HCC1569, HCC1419, BT474, UACC893, CRL2351 and HCC1954. UACC893, MDA468 and BT474 cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS) (Hyclone), and 1% penicillin/streptomycin (P/S). All other cancer cell lines (HCC38, HCC1937, HCC1395, HCC70, HCC1806, HCC1143, HCC202, HCC1569, HCC1419, CRL2351, HCC1954 and MDA231) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Invitrogen), supplemented with 10% FBS and 1% P/S. MCF10A cells were grown in DMEM/F12 medium supplemented with 5% horse serum, 10 μg/ml insulin, 20 ng/ml EGF, 0.5 μg/ml hydrocortisone, 100 ng/ml Cholera toxin and 1% P/S. Cells were grown at 37°C in 5% CO2. Retroviral and lentiviral infections were performed as previously described [35 (link)].

Zhao H., Faltermeier C.M., Mendelsohn L., Porter P.L., Clurman B.E, & Roberts J.M. (2015). Mislocalization of p27 to the cytoplasm of breast cancer cells confers resistance to anti-HER2 targeted therapy. Oncotarget, 5(24), 12704-12714.

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Diverse Human Cell Line Cultures

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293T (CRL-3216), MCF10A (CRL-10317), T47D (HTB-133), MCF7 (HTB-22), MDAMB231 (CRM-HTB-26), MDAMB468 (HTB-132), BT549 (HTB-122), HCC1500 (CRL-2329), HCC1569 (CRL-2330) and HCC1806 (CRL-2335) were purchased from American Type Culture Collection (ATCC). HCC70 and MDAMB175 were provided by Wake Forest Institutional Cell Bank Repository. Human pre-adipocytes BR-F was obtained from Zen-Bio (BR-F). Human preadipocytes cell line SGBS was a kind gift from Dr. Martin Wabitsch (Ulm University Medical Center). MCF10A was cultured as described in human mammary epithelial growth medium (Lonza)^{78 (link)}. 293T and breast cancer cell lines MCF-7, were cultured in DMEM (Gibco) with 10% fetal bovine serum (FBS). MDAMB468 and MDAMB175-VII was cultured in L15 medium (Gibco) with 10% FBS. MDAMB231, BT549, HCC70, HCC1500, HCC1569, HCC1806, T47D, were cultured in RPMI 1640 (Gibco) with 10% FBS. BR-F was cultured in Preadipocyte Medium (PM-1, Zen-Bio). SGBS was cultured in 0F (DMEM/F12 + pantothenate +biotin + penicillin/streptomycin) plus 10% FBS. All cell lines were authenticated and were regularly tested for mycoplasma contamination.

Zhao D., Wu K., Sharma S., Xing F., Wu S.Y., Tyagi A., Deshpande R., Singh R., Wabitsch M., Mo Y.Y, & Watabe K. (2022). Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytes. Nature Communications, 13, 7734.

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Culturing Breast and Kidney Cell Lines

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The basal‐like breast cancer cell lines MDA‐MB‐468, HCC70, HCC38, the luminal breast cancer line MCF7, and the human embryonic kidney (HEK) 293T cells were originally obtained from the American Type Culture Collection (USA). MDA‐MB‐468, HCC70, HCC38, and MCF7 were grown in RPMI, while HEK293T cells were grown in Dulbecco's modified Eagle medium (DMEM) (Gibco BRL, USA). Unless otherwise indicated, all cell lines were cultured in a medium containing 10% fetal bovine serum (Gibco BRL, USA), L‐Glutamine (2 mM), and penicillin/streptomycin. Cells were cultured at 37 °C in a humidified incubator of 5% CO₂. Cell lines were routinely (once a month) checked for mycoplasma using an RT‐PCR.

Vinik Y., Maimon A., Dubey V., Raj H., Abramovitch I., Malitsky S., Itkin M., Ma'ayan A., Westermann F., Gottlieb E., Ruppin E, & Lev S. (2024). Programming a Ferroptosis‐to‐Apoptosis Transition Landscape Revealed Ferroptosis Biomarkers and Repressors for Cancer Therapy. Advanced Science, 11(17), 2307263.

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Breast Cancer Cell Line Culturing Protocols

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MCF-7, HCC70, MDA-MB-231, MDA-MB-436 and MDA-MB-468 BC cells were obtained from the American Type Culture Collection (LGC Standards, Middlesex, UK) and were authenticated by Cancer Research UK (London, UK). MCF-7 and MDA-MB-468 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, Madrid, Spain) supplemented with 10% foetal calf serum (FCS), 4 mM glutamine and 100 U/mL penicillin/streptomycin (Sigma-Aldrich, Madrid, Spain). HCC70 cells were cultured in RPMI 1640 (Gibco, Madrid, Spain) supplemented with 10% foetal calf serum. MDA-MB-231 cells were grown in phenol red-free DMEM (Invitrogen, Barcelona, Spain) supplemented with 10% foetal calf serum (FCS), 100 Units/mL penicillin, 100 Units/mL streptomycin and 2 mM L-glutamine. MDA-MB-436 cells were grown in Leibovitz’s L-15 medium (Gibco, Madrid, Spain) supplemented with 10% horse serum (Invitrogen, Barcelona, Spain). All cell lines were grown in an atmosphere of 10% CO₂ at 37 °C except MDA-MB-436 cells, which were grown without CO₂ at 37 °C.

Roig B., Rodríguez-Balada M., Samino S., Lam E.W., Guaita-Esteruelas S., Gomes A.R., Correig X., Borràs J., Yanes O, & Gumà J. (2017). Metabolomics reveals novel blood plasma biomarkers associated to the BRCA1-mutated phenotype of human breast cancer. Scientific Reports, 7, 17831.

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Culture of TNBC Cell Lines

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The human TNBC cell lines BT20 and HCC70, as well as 293T cells, were both obtained from Cell Bank of the Chinese Academy of Science. BT20 and HCC70 were cultured in RPMI 1640 medium, while 293T was cultured in DMEM (Gibco), both added with 10% fetal calf serum (FBS) (Invitrogen) at 37°C with 5% CO₂.

Yang W., Cui G., Ding M., Yang M, & Dai D. (2020). MicroRNA‐124‐3p.1 promotes cell proliferation through Axin1‐dependent Wnt signaling pathway and predicts a poor prognosis of triple‐negative breast cancer. Journal of Clinical Laboratory Analysis, 34(7), e23266.

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Cell Line Maintenance Protocol

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Cell lines including 4T1, CT26, Py8119, ACHN, THP-1, and Jurkat cells were purchased from American Type Culture Collection (ATCC). HCC70, HCC1419, and HCC1937 were gifts from Dr. Peggy Porter, Fred Hutchinson Cancer Research Center (FHCRC). 4T1, CT26, HCC70, HCC1419, HCC1937 cells were maintained in RPMI1640 media (Gibco by Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS), 1% Penicillin-Streptomycin (P/S, final concentration: 100 units/ml of penicillin, 100 μg/ml of streptomycin) (Gibco). Py8119 cells were maintained in F-12K medium (ATCC) with 5% FBS. THP-1 cells were cultured in RPMI1640 media supplemented with 10% FBS, 1% P/S, 1 mM sodium pyruvate (Lonza, Basel, Switzerland), and 55 nM β-mercaptoethanol (Gibco). Jurkat cells were maintained in RPMI1640 media with 10% FBS, 1% P/S, 10 mM HEPES (Santa Cruz Biotechnology, Dallas, TX, USA), and 1 mM sodium pyruvate. All cells were cultured in humidified 37°C incubators with 5% CO₂ atmosphere.

Nishida-Aoki N, & Gujral T.S. (2021). Polypharmacological reprogramming of tumor-associated macrophages towards an inflammatory phenotype. Cancer research, 82(3), 433-446.

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