Quantstudio 7 real time pcr software

Manufactured by Thermo Fisher Scientific

Sourced in United States

The QuantStudio™ 7 Real Time PCR software is a data analysis application used in conjunction with Thermo Fisher Scientific's real-time PCR instruments. The software enables the collection, analysis, and reporting of quantitative PCR data.

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Lab products found in correlation

Taqman universal master mix 2 with ung, by Thermo Fisher Scientific (1 mentions) Rneasy plus mini extraction kit, by Qiagen (1 mentions) Quantstudio 7 flex real time pcr system, by Thermo Fisher Scientific (1 mentions) Taqman assay, by Thermo Fisher Scientific (1 mentions) Quantitect reverse transcription kit, by Qiagen (1 mentions) Applied biosystems taqman snp genotyping assay, by Thermo Fisher Scientific (1 mentions)

2 protocols using quantstudio 7 real time pcr software

Quantitative Real-Time RT-PCR Analysis of Ovarian Tissue

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RNA was extracted from whole ovaries or tumour pieces using RNeasy Plus mini extraction kit (Qiagen, Germany) and reverse transcription performed using QuantiTect Reverse transcription kit (Qiagen, Germany). All quantitative real time rtPCR assays were carried out three times using TaqMan® universal master mix II with UNG (Applied Biosystems, USA), Taqman® assays (Additional file 1: Table S3) and QuantStudio™ 7 Flex Real Time PCR system (ThermoFisher, USA), and relative expression levels determined using QuantStudio™ 7 Real Time PCR software.

Mohamed N.E., Hay T., Reed K.R., Smalley M.J, & Clarke A.R. (2019). APC2 is critical for ovarian WNT signalling control, fertility and tumour suppression. BMC Cancer, 19, 677.

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CTSB Variant Genotyping in PD Cohorts

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Genotyping was performed in 5 different cohorts (2077 PD cases and 615 controls) collected from independent sites (Table 2). All individuals were of European Non-Finnish descent. The study was approved by the ethical review board from each institution and all participants provided informed written consent. A custom Applied Biosystems Taqman SNP Genotyping Assay (Thermo Fisher Scientific, Waltham, MA, USA) was designed for CTSB c.851G > T p.Gly284Val (NM_001908). Genotyping was performed using a QuantStudio 7 Real-Time PCR system. QuantStudio™ 7 Real-Time PCR Software (Thermo Fisher Scientific, Waltham, MA, USA) was used for analysis.

Milanowski L.M., Hou X., Bredenberg J.M., Fiesel F.C., Cocker L.T., Soto-Beasley A.I., Walton R.L., Strongosky A.J., Faroqi A.H., Barcikowska M., Boczarska-Jedynak M., Dulski J., Fedoryshyn L., Janik P., Potulska-Chromik A., Karpinsky K., Krygowska-Wajs A., Lynch T., Olszewska D.A., Opala G., Pulyk A., Rektorova I., Sanotsky Y., Siuda J., Widlak M., Slawek J., Rudzinska-Bar M., Uitti R., Figura M., Szlufik S., Rzonca-Niewczas S., Podgorska E., McLean P.J., Koziorowski D., Ross O.A., Hoffman-Zacharska D., Springer W, & Wszolek Z.K. (2022). Cathepsin B p.Gly284Val Variant in Parkinson’s Disease Pathogenesis. International Journal of Molecular Sciences, 23(13), 7086.

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