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Vyntus

Manufactured by Vyaire Medical
Sourced in United States

The Vyntus is a comprehensive pulmonary function testing system designed to provide accurate and reliable measurements of lung function parameters. It is capable of performing a variety of respiratory tests, including spirometry, diffusion capacity, and body plethysmography. The Vyntus is equipped with advanced technology and meets industry standards for precision and reliability in pulmonary function assessment.

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4 protocols using vyntus

1

Cardiovascular and Respiratory Monitoring During Exercise

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Heart rate (HR) and electrocardiography were monitored and recorded continuously by a wireless belt placed around the chest at the level of the heart (Equivital, Equivital Inc, New York, USA). Beat-by-beat HR was derived from each RR interval (RRI) identified from QRS complexes from continuous electrocardiogram. Blood pressure was monitored and continuously recorded via arterial catheterization of the radial artery, with transducers zeroed and placed at the level of the heart Breath-by-breath gas exchange parameters were monitored during the exercise protocol by indirect calorimetry (Vyntus, Vyaire Medical, Mettawa IL, USA) to oxygen uptake (VO2).
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2

Determining Maximal Oxygen Uptake and Peak Power

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Participants performed an incremental exercise test to task failure to determine V̇o2peak and Wmax on a cycle ergometer (Excalibur Sport; Lode, Groningen, the Netherlands), as previously described (22 (link)). In brief, the test started at a power output of 100 W and the workload increased by 30 W every 2 min. During the test, gas exchange measurements were made using an automated online gas analysis system (Vyntus, Vyaire Medical, Ottawa, IL). The highest 30-s average of O2 uptake was considered to represent V̇o2peak. Wmax was calculated as the power output from the last completed stage plus the fraction of the time spent in the next stage multiplied by 30 W.
Participants meeting the study’s minimal V̇o2peak criteria were then scheduled for the familiarization trial. This trial differed from the experimental trials in that only one muscle biopsy was taken, and this was before the glycogen-reducing exercise session to obtain an indication of typical resting glycogen concentration.
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3

Incremental Cycling Test for VO2 peak

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Participants performed an incremental test to exhaustion to determine V O 2 peak and Wmax on a cycle ergometer (Lode, Groningen, Netherlands). The test started at an intensity of 100 W and the workload increased by 30 W every 2 minutes. During the test, gas exchange measurements were made using an automated online gas analysis system (Vyntus, Vyaire Medical, IL, US). The highest 30-s average of O 2 uptake was considered to represent V O 2 peak. Wmax was calculated as the power output from the last completed stage plus the fraction of the time spent in the next stage multiplied by 30 W.
Participants were then scheduled for the familiarisation trial that, with the exception of blood sampling, followed the same protocol as the PLA trial (see below).
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4

Cardiorespiratory Fitness Assessment Protocol

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Participants attended a screening visit and gave their written informed consent before completing a general health questionnaire. Participants’ height (Stadiometer Model 220, Seca, Germany) and body mass (Champ II, OHAUSE, Switzerland) were recorded before exercise. Participants mounted a cycle ergometer (Lode Excalibur Sport, Groningen, The Netherlands) and adjusted the saddle and handlebar positioning until comfortable, which was replicated for subsequent visits. They then performed a step-incremental exercise test to exhaustion, which commenced at 100 W and was increased by 30 W every 2 min until volitional exhaustion, or until a cadence of >50 rpm could not be maintained. Indirect calorimetry was performed throughout exercise using an online automated gas analyzer (Vyntus, Vyaire Medical, Mettawa, IL), to determine V̇o2 and V̇co2. The volume transducer, CO2, and O2 sensors were calibrated before each measurement as per the manufacturer’s instructions. Heart rate (HR) was measured continuously via telemetry (Polar H7, Kempele, Finland). Peak power was calculated as the power output of the last completed stage, added to the fraction of the time spent in the following stage, multiplied by 30 W. V̇o2peak was calculated as the highest 30 s average of V̇o2.
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